Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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siRNA Interference with a Proliferation-Inducing Ligand Gene in the Sgr-7901 Gastric Carcinoma Cell Line

  • Ni, Shao-Zhong (Department of General Surgery, First Affiliated Hospital, Nanjing Medical University) ;
  • Cao, Hong-Yong (Department of General Surgery, Affiliated Nanjing First Hospital, Nanjing Medical University) ;
  • Chen, Zheng (Department of General Surgery, First Affiliated Hospital, Nanjing Medical University) ;
  • Zhu, Yi (Department of General Surgery, First Affiliated Hospital, Nanjing Medical University) ;
  • Xu, Ze-Kuan (Department of General Surgery, First Affiliated Hospital, Nanjing Medical University)
  • Published : 2012.04.30
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Abstract

Objective: The present study aimed to investigate the influence of siRNA interference with a proliferation-inducing ligand (APRIL) gene on gastric carcinoma sgr-7901 cell apoptosis. Correlations between APRIL silencing and tyrosine kinase (trka) expression were also explored. Methods: Two APRIL-silencing siRNA vectors were constructed, and transfected into human gastric carcinoma sgr-7901 cells, expression before and after transfection being detected using RT-PCR and western blot analyses. The expression of 15 trka genes was detected using RT-PCR and apoptotic rates of sgr-7901 were assessed by flow cytometry. Results: The expression levels of receptor trka genes were significantly decreased, and the apoptotic rate of sgr-7901 was significantly increased after transfection (P < 0.05). Conclusion: APRIL gene silencing can increase the apoptotic rate of gastric carcinoma cells, and inhibit the expression of receptor trka genes. There is a correlation between the signaling pathways of APRIL and trka.

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