Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Fucoidan Upregulates Chemotactic Activity of Canine Peripheral Blood Polymorphonuclear Cells Through Interleukin-8 from Peripheral Blood Mononuclear Cells in vitro

개 말초혈액 다형핵백혈구의 유주활성에 있어 fucoidan의 효과

  • Jeon, Chun-Jin (Laboratory of Veterinary Internal Medicine, Department of Veterinary Medicine, College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Kim, Soo-Hyun (Laboratory of Veterinary Internal Medicine, Department of Veterinary Medicine, College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Kim, Sung-Soo (Laboratory of Veterinary Internal Medicine, Department of Veterinary Medicine, College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Kang, Ji-Houn (Laboratory of Veterinary Internal Medicine, Department of Veterinary Medicine, College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Yang, Mhan-Pyo (Laboratory of Veterinary Internal Medicine, Department of Veterinary Medicine, College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
  • Accepted : 2012.06.11
  • Published : 2012.06.30
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Abstract

Fucoidan has been shown to enhance immune function. The objective of this study was to examine the in vitro effect of fucoidan on the chamotactic activity of canine peripheral blood polymorphonuclear cells (PMNs). The chemotactic activity of PMNs was evaluated by method of a modified Boyden chamber assay. The amount of interleukin (IL)-8 in the culture supernatants from peripheral blood mononuclear cells (PBMCs) treated with fucoidan was determined by means of ELISA. Fucoidan itself could not have chemoattract effects for PMNs. However, the chemotaxis of PMNs was remarkably enhanced by culture supernatant from PBMCs treated with fucoidan. Similarly, it was also increased by recombinant canine (rc) IL-8. These chemotactic activities of PMNs were inhibited by addition of anti-rcIL-8 polyclonal antibody (pAb). The amount of IL-8 in the culture supernatant from PBMCs was shown to increase upon treatment of fucoidan as compared with that of untreated PBMCs culture supernatant. These results suggest that fucoidan upregulates the chemotaxis of PMNs, which is mainly mediated by IL-8 released from fucoidanstimulated PBMCs.

개 말초혈액 다형핵백혈구(PMNs)의 유주활성에 대한 fucoidan의 면역증효과를 검토하였다. Fucoidan 그 자체는 PMNs에 대해 직접적인 유주활성 효과를 보이지 않았다. 그러나 recombinant IL-8에 대한 PMNs의 유주활성과 유사하게 fucoidan으로 배양한 말초혈액 단핵구세포(PBMCs)의 배양상층액은 PMNs에 대해 유주활성을 증가시켰다. 또한, fucoidan으로 배양한 PBMCs의 배양상층액에 대한 PMNs의 유주활성 증가효과는 anti-IL-8 pAb를 처리했을 때 억제되었다. PBMCs 배양 상층액 속의 IL-8의 양을 정량한 결과 fucoidan무처치 대조군에 비해 증가하였다. 이상의 결과로부터, fucoidan은 개 PMNs의 유주능에 대하여 면역증가 작용을 가지고 있으며, 이것은 fucoidan의 자극에 의해 PBMCs에서 생산되어 분비되는 IL-8에 의해 나타나는 것으로 사료되었다.

Keywords

References

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