Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Biological Assay of Mercury and Cadmium Ions Using DNA Immobilized on a Nanotube Paste Electrodes

  • Ly, Suw-Young (Department of fine Chemistry, Seoul National University of science & Technology) ;
  • Lee, Chang-Hyun (Division of General Education, Pyeongtaek University) ;
  • Jung, Hong-Rak (Advanced Scientific Experiment Group in Daejin High School) ;
  • Park, Kwang-Ho (Advanced Scientific Experiment Group in Daejin High School) ;
  • Park, Yong-Keun (Advanced Scientific Experiment Group in Daejin High School) ;
  • Suk, Hong-Woo (Advanced Scientific Experiment Group in Daejin High School)
  • Received : 2012.06.05
  • Accepted : 2012.06.20
  • Published : 2012.06.30
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Abstract

Bio assay of mercury and cadmium ions were searched using voltammetric analysis using DNA doped carbon nanotube paste electrodes (DCP). The square-wave stripping voltammetryic optimized results indicated working ranges of 1-10.0 $ngL^{-1}$ and 20-100 $ugL^{-1}$, Hg(II) Cd(II) within an accumulation time of 120 seconds, in 0.1-M phosphate buffer solutions of pH 6.3. The relative standard deviations of 5 $ngL^{-1}$ Hg(II) and Cd(II) that observed were 0.14 and 0.22% (n=12), respectively, using optimum conditions. The low detection limit (S/N) was pegged at 0.1 $ngL^{-1}$ ($4.9{\times}10^{-11}M$) Hg(II) and 0.2 $ngL^{-1}$ ($1.77{\times}10^{-10}M$) Cd(II). The developed methods can be applied to assays in biological fish kidneys and water samples.

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References

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