Korean Journal of Organic Agriculture (한국유기농업학회지)

Korean Association of Organic Agriculture (한국유기농업학회)
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In vitro Rumen Fermentation Patterns of Environment Friendly Whole Crop Barley, Italian Ryegrass and Rice Straw Silages

친환경 청보리, 이탈리안 라이그라스, 볏짚사일리지의 In vitro 반추위 발효성상 비교연구

  • Mbiriri, David Tinotenda (Department of Animal Science, Chonbuk National University) ;
  • Oh, Sung-Jin (Department of Animal Science, Chonbuk National University) ;
  • Lee, A-Reum (Department of Animal Science, Chonbuk National University) ;
  • Chae, Jung-Il (Department of Oral Pharmacology, School of Dentistry and Institute of Dental Bioscience, Chonbuk National University) ;
  • Choi, Chang-Weon (Department of Animal Resources, Daegu University) ;
  • Choi, Nag-Jin (Department of Animal Science, Chonbuk National University)
  • Received : 2012.06.04
  • Accepted : 2012.06.21
  • Published : 2012.06.30
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Abstract

Rumen fermentative characteristic is useful indicators of the quality of ruminant feed stuffs and diets. An in vitro rumen fermentation experiment was therefore carried out to compare fermentation patterns among three forage sources. These were whole crop barley (WCBS), Italian ryegrass silage (IRGS) and rice straw silages (RSS). Rice straw (RS) was the control, making the treatments 4 in total. Forages were randomly allocated to serum bottles. The incubation times were arranged 0, 3, 6, 9, 12, 24, 48 and 72h at $39^{\circ}C$, respectively. Each forage source was replicated 3 times per incubation time. At each sampling time, total gas and pH were measured, whilst individual volatile fatty acids (VFAs), total volatile fatty acids (TVFAs) and ammonia nitrogen ($NH_3$-N) were determined later after storing samples at $-20^{\circ}C$. Acetate: Propionate ratio (A/P) was then calculated. Forage source had a significant effect (P<0.001) on pH and $NH_3$-N. RSS maintained higher pH values than the rest of the forage sources. A decreasing pH trend with increased time of incubation, in agreement with literature, was observed for all forage sources. WCBS recorded $NH_3$-N values higher than all the other treatments. Total gas, individual and total VFA and A/P ratio were not affected by forage source. However, there was a significant difference in all parameters (p<0.05) among forage sources at sampling periods at 3 to 72h. Therefore, the present results indicating that WCBS, IRGS, RS and RSS maintained in vitro rumen pH above the critical value. Also, WCBS produced the highest NH3-N and on this merit could be of better nutritive value, in vivo, in the ruminant.

Keywords

References

  1. Beever D. E. and F. L. Mould. 2000. Forage evaluation for efficient ruminant livestock production. In: Forage evaluation in ruminant nutrition. Givens D. I., Owen E., Axford R. F. E. and Omed H. M. (Editors). CAB Publishing. 15-42.
  2. Chaney A. L. and E. P. Marbach. 1962. Modified reagents for the determination of urea and ammonia. Clin. Chem. 8: 130.
  3. Erwin E. S., J. Marco, and E. M. Emery. 1961. Volatile fatty acid analysis of blood and rumen fluid by gas chromatography. J. Dairy Sci. 44: 1768. https://doi.org/10.3168/jds.S0022-0302(61)89956-6
  4. France, F. and R. C. Siddons. 1993. Volatile fatty acid production. Pages 107-121 in Quantitative Aspects of Ruminant Digestion and Metabolism. J. M. Forbes, ed. CAB Int., Wallingford, UK.
  5. Gao, L. J., H. Y. Yang, X. F. Wang, Z. Y. Huang, Masaharu Ishii, Yasuo Igarashi, and Z. J. Cui. 2008. Rice straw fermentation using lactic acid bacteria. Biores. Tech. 99: 2742-2748. https://doi.org/10.1016/j.biortech.2007.07.001
  6. Gizzi, G., R. Zanchi, and F. Sciaraffia. 1998. Comparison of microbiological and fermentation parameters obtained with an improved rumen in vitro technique with those obtained in vivo. Anim. Feed Sci. Technol. 73: 291-305. https://doi.org/10.1016/S0377-8401(98)00150-3
  7. Hiltner, P. and B. A. Dehority. 1983. Effect of soluble carbohydrates on digestion of cellulose by pure cultures of rumen bacteria. Applied and Environmental Microbiology 46: 642-648
  8. Mansfield, H. R., M. I., Endres, M. D. Stern. 1995. Comparison of microbial fermentation in the rumen of dairy cows and dual flow continuous culture. Anim. Feed Sci. Technol. 55, 47-66. https://doi.org/10.1016/0377-8401(95)98202-8
  9. Menke K. H., L. Raab, A. Salewski, H. Steingass, D. Fritz, and W. Schneider, 1979. The estimation of digestibility and metabolisable energy content of ruminant feeding-stuffs from the gas production when they are incubated with rumen liquor in vitro. J. Agric. Sci. (Camb.) 93: 217-222. https://doi.org/10.1017/S0021859600086305
  10. Mould, F. L., K. E. Kleim, R. Morgan, R. M. Mauricio. 2005. In vitro microbial inoculum: a review of its function and properties. Anim. Feed Sci. Technol. 123/124: 31-50. https://doi.org/10.1016/j.anifeedsci.2005.04.028
  11. Owens F. N. and A. L. Goetsch. 1993. Ruminant fermentation. In: The ruminant Animal: Digestive Physiology and Nutrition. Waveland Press, 145-171.
  12. Perdok, H. and R. A. Leng. 1989. Rumen ammonia requirements for efficient digestion and intake of straw by cattle. In: The Roles of Protozoa and Fungi in ruminant Digestion (Ed. J. V. Nolan and R. A. Leng), Penambul Books, Armidale, Australia.
  13. Sarnklong, C., J. W. Cone, W. Pellikaan, and W. H. Hendriks. 2010. Utilisation of rice straw and different treatments to improve its feed value for ruminants: A review. Asian- Aust. J. Anim. Sci. 23: 680-692. https://doi.org/10.5713/ajas.2010.80619
  14. Stewart, C. S. 1977. Factors Affecting the Cellulolytic Activity of Rumen Contents. Appl. Environ. Microbiol. 33: 497-502.
  15. Tilley, J. M. A. and R. A. Terry. 1963. A two stage technique for the in vitro digestion of forage crops. J. Brit. Grassland Society. 18: 104-111. https://doi.org/10.1111/j.1365-2494.1963.tb00335.x
  16. Troelsen, J. E., and H. J. Donna. 1966. Ruminant digestion in vitro as affected by inoculum donor collection day, and fermentation time. Can. J. Anim. Sci. 46: 149-156. https://doi.org/10.4141/cjas66-022
  17. Van Saun, R. J. 2007. Forage testing: How to translate numbers into actions. In: Proceedings of 2006 Mid-Atlantic States Conference for Bovine Practitioners, Frederick, Maryland, March 30-31, 2006, 13pp. http://vbs.psu.edu/extension/resources-repository/publications/Forage%20Testing-MAC06.pdf