DOI QR코드

DOI QR Code

Immunomodulatory Effects of Fucoidan on NK Cells in Ovariectomized Rats

  • NamKoong, Seung (Department of Physical Therapy, Kangwon National University) ;
  • Kim, Ye-Jin (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Kim, Tae-Seong (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Sohn, Eun-Hwa (Department of Herbal Medicine Resource, Kangwon National University)
  • Received : 2012.05.29
  • Accepted : 2012.06.25
  • Published : 2012.06.30

Abstract

In this study we investigated the effects of supplementation with fucoidan from brown alga on the function of natural-killer (NK) cells to evaluate the possibility as an immunomodulator in ovariectomized (OVX) rats. A total of 18 female Wistar rats (six weeks) were used this study and 12 rats were OVX, and the rest of rats were sham-operated. The sham and one OVX group were fed standard diet, and the remaining OVX group received fucoidan (0.05% supplemented diet). After 12 weeks of supplementation, rats were sacrificed to assess the tumoricidal activity of the NK cells and the NO-iNOS regulation from splenocytes. The mass of body and the immune organs such as spleen and thymus were also studied. In OVX rats, body and thymus weights increased, however fucoidan supplementation did not change the body mass and organs weight compared to OVX group. Fucoidan supplementation increased NK cell activity and reduced NO-iNOS production in OVX rats. Ex vivo treatment of fucoidan increased NK cell activity in splenocytes from shame and OVX rats. Ex vivo, we confirmed that fucoidan partially reduced the NK cell activity in the presence of iNOS inhibitors in OVX-splenocytes. These results indicate fucoidan supplementation has a NK cell tumoricidal activity, which are regulated by the iNOS production in OVX rats. This suggests that fucoidan is useful for potential therapeutic strategies as a nutrient in regulating the NK cells in postmenopausal osteoporosis patients.

Keywords

References

  1. Baba, M., R. Snoeck, R. Pauwels and E. de Clercq. 1988. Sulfated polysaccharides are potent and selective inhibitors of various enveloped viruses, including herpes simplex virus, cytomegalovirus, vesicular stomatitis virus, and human immunodeficiency virus. Antimicrob. Agents Chemother. 32: 1742-1745. https://doi.org/10.1128/AAC.32.11.1742
  2. Baral, E., E. Nagy and I. Berczi. 1995. Modulation of natural killer cell-mediated cytotoxicity by tamoxifen and estradiol. Cancer 75:591-599. https://doi.org/10.1002/1097-0142(19950115)75:2<591::AID-CNCR2820750224>3.0.CO;2-U
  3. Borrego, F., M.C. Alonso, M.D. Galiani, J. Carracedo, R. Ramirez, B. Ostos, J. Pena and R. Solana. 1999. NK phenotypic markers and IL-2 response in NK cells from elderly people. Exp. Gerontol. 34:253-265. https://doi.org/10.1016/S0531-5565(98)00076-X
  4. Choi, H.S., K.H. Kim, E. Sohn, J.D. Park, B.O. Kim, E.Y. Moon, D.K. Rhee and S. Pyo. 2008. Red ginseng acidic polysaccharide (RGAP) in combination with IFN-${\gamma}$ results in enhanced macrophage function through activation of the NF-${\kappa}$B pathway. Biosci. Biotechnol. Biochem. 72:1817-1825. https://doi.org/10.1271/bbb.80085
  5. Jang, S.A., S. Namkoongm and E.H. Sohn. 2010 Effects of ${\beta}$ -glucans from Coriolus versicolor on macrophage functions in young and aged mice. J. Korean Plant Res. 23(6): 498- 502.
  6. Kalu, D.N. 1991. The ovariectomized rat model of postmenopausal bone loss. Bone Miner. 15:175-192. https://doi.org/10.1016/0169-6009(91)90124-I
  7. Kalu, D.N., C.C. Liu, E. Salemo, B. Hollin, R. Echon and M. Ray. 1991. Skeletal response of ovariectomized rats to low and high doses of 17 ${\beta}$-estradiol. Bone Miner. 14:175-187. https://doi.org/10.1016/0169-6009(91)90021-Q
  8. Kim, K.S., S. Pyo and E.H. Sohn. 2009. Immunomodulation of NK Cell activity by red ginseng acidic polysaccharide (RGAP) in ovariectomized rats. J. Ginseng Res. 33(2):99-103. https://doi.org/10.5142/JGR.2009.33.2.099
  9. Levy, S.M., J. Fernstrom, R.B. Herberman, T. Whiteside, J. Lee, M. Ward and M. Massoudi. 1991. Persistently low natural killer cell activity and circulating levels of plasma beta endorphin: risk factors for infectious disease. Life Sci. 48:107-116. https://doi.org/10.1016/0024-3205(91)90403-X
  10. Mahony, M.C., S. Oehninger, G.F. Clark, A.A. Acosta and G.D. Hodgen. 1991. Fucoidin inhibits the zona pellucidainduced acrosome reaction in human spermatozoa. Contraception. 44:657-665. https://doi.org/10.1016/0010-7824(91)90085-T
  11. Miller, R.A. 1991. Aging and immune function. Int. Rev. Cytol. 124:187-215. https://doi.org/10.1016/S0074-7696(08)61527-2
  12. Mocchegiani, E. and M. Malavolta. 2004. NK and NKT cell functions in immunosenescence. Aging Cell. 3:177-184. https://doi.org/10.1111/j.1474-9728.2004.00107.x
  13. Mosmann, T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65:55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  14. Nakaya, M., H. Tachibana and K. Yamada. 2006. Effect of estrogens on the interferon-γ producing cell population of mouse splenocytes. Biosci. Biotechnol. Biochem. 70:47-53. https://doi.org/10.1271/bbb.70.47
  15. Namkoong S., E.S. Cheoung, H. Joo, S.A. Jang, Y.J. Yang, S.C. Kang, K.H. Jang and E.H. Sohn. 2011. Immunomodulatory effects of Eisenia bicyclis on innate immune cells in acute exercise-stress rat model. J. Korean Plant Res. 24(3):286-291. https://doi.org/10.7732/kjpr.2011.24.3.286
  16. O'Leary, R., M. Rerek and E.J. Wood. 2004. Fucoidan modulates the effect of transforming growth factor (TGF)-${\beta}$1 on fibroblast proliferation and wound repopulation in in vitro models of dermal wound repair. Biol. Pharm. Bull. 27:266- 270. https://doi.org/10.1248/bpb.27.266
  17. Patel, M.K., B. Mulloy, K.L. Gallagher, L. O'Brien and A.D. Hughes. 2002. The antimitogenic action of the sulphated polysaccharide fucoidan differs from heparin in human vascular smooth muscle cells. Thromb. Haemost. 87:149-154.
  18. Sohal, R.S. and R.G. Allen. 1990. Oxidative stress as a causal factor in differentiation and aging: a unifying hypothesis. Exp. Gerontol. 25:499-522. https://doi.org/10.1016/0531-5565(90)90017-V
  19. Wronski, T.J., P.A. Schenk, M. Cintron and C.C. Walsh. 1987. Effect of body weighton osteopenia in ovariectomized rats. Calcif. Tissue Int. 40:155-159. https://doi.org/10.1007/BF02555700
  20. Yamaguchi, M. 2006. Regulatory mechanism of food factors in bone metabolism and prevention of osteoporosis. Yakugaku Zasshi. 126:1117-1137. https://doi.org/10.1248/yakushi.126.1117
  21. Yovel, G., K. Shakhar and S. Ben-Eliyahu. 2001. The effects of sex, menstrual cycle, and oral contraceptives on the number and activity of natural killer cells. Gynecol. Oncol. 81:254- 262. https://doi.org/10.1006/gyno.2001.6153

Cited by

  1. Dietary Supplementation with Low-Molecular-Weight Fucoidan Enhances Innate and Adaptive Immune Responses and Protects against Mycoplasma pneumoniae Antigen Stimulation vol.17, pp.3, 2012, https://doi.org/10.3390/md17030175