Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Study on the Antioxidative and Physiological Activities of Saururus chinensis Extract

삼백초(Saururus chinensis) 추출물의 항산화능 및 생리활성 연구

  • Kang, Chang-Soo (Department of Biological Science and the Research Institute for Basic Sciences, Hoseo University) ;
  • Lee, Min-Joo (Department of Biological Science and the Research Institute for Basic Sciences, Hoseo University) ;
  • Park, Cheol-Bem (Fusion Technology, Hoseo University) ;
  • Bang, In-Seok (Department of Biological Science and the Research Institute for Basic Sciences, Hoseo University)
  • Received : 2012.03.24
  • Accepted : 2012.06.06
  • Published : 2012.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Saururus chinensis has long been widely used in oriental folk medicines to treat diseases. In the current study, organic solvent fractions obtained from the main methanolic extract of S chinensis were evaluated for their antioxidative and related physiological activities. The antioxidant activity of the fractions was measured using DPPH free radical scavenging activity, increased in a dose-dependent manner, and the $ED_{50}$ of the ethyl acetate fractions exhibited a value of 12.84 ${\mu}g/ml$ higher than 27.22 ${\mu}g/ml$ compared to the BHT. Also, the cell viability of S. chinensis on $H_2O_2$-induced HDF cell death ($IC_{50}$) showed the highest cell viability of 89.39% in 50 ${\mu}g/ml$ of ethyl acetate fraction and 67.98% of visible cell survival rate in n-butanolic fraction. Meanwhile, all fractions of the S. chinensis extract led to a slight down regulation of the mRNA expression of fibulin-5, which is related to skin elasticity, and the ethyl acetate fraction having high antioxidant activity showed a markedly inhibitory effect on chick embryonic angiogenesis using the CAM assay. These results suggest that the ethyl acetate fraction of S. chinensis extract could be a good material in therapeutic application for antioxidant and related anti-angiogenesis activities.

약용소재로 사용되고 있는 삼백초의 잎과 줄기로부터 유기 용매의 극성에 따른 순차적 분획물을 얻어 항산화 효과와 연관하여 피부의 탄력 및 혈관형성에 미치는 영향을 조사하였다. 삼백초 추출 분획물에 대한 항산화 활성은 시료의 농도가 증가할수록 DPPH에 대한 전자공여능도 증가하였으며, $ED_{50}$은 EtOAc 분획에서 합성 항산화제인 BHT의 27.22 ${\mu}g/ml$보다 12.84 ${\mu}g/ml$로 높게 나타났다. 또한 $H_2O_2$에 의해 유도된 HDF 세포사멸($IC_{50}$)에 대하여 삼백초 추출 50 ${\mu}/ml$의 EtOAc 분획에서 89.39%의 가장 높은 세포 생존율을 나타내었으며, 동량의 n-BuOH 분획에서도 67.98%의 유의적인 세포 생존율을 보였다. 한편 삼백초 추출 분획물은 피부 탄력과 관련있는 fibulin-5 mRNA의 발현율을 감소시켰으며, 항산화 활성이 높은 삼백초 추출 EtOAc 분획에 대한 CAM assay 결과, 혈관형성을 억제하는 것으로 나타났다. 이러한 삼백초 추출 EtOAc 분획은 향후 항산화 활성 및 해독과 관련된 혈관형성 억제의 약재개발 가능성이 있음을 암시하는 것으로 이의 작용기작에 대한 더욱 자세한 연구가 필요하다고 생각한다.

Keywords

References

  1. Aberer, W., Anderson, K. E. and White, I. R. 1993. Should patch testing be restricted to dermatologists only. Contact Dermatitis 28, 1-2. https://doi.org/10.1111/j.1600-0536.1993.tb03314.x
  2. Albig, A. R. and Schiemann, W. P. 2004. Fibulin-5 antagonizes vascular endothelial growth factor (VEGF) signaling and angiogenic sprouting by endothelial cells. DNA Cell Biol. 23, 367-379. https://doi.org/10.1089/104454904323145254
  3. Albig, A. R. and Schiemann, W. P. 2005. Fibulin-5 function during tumorigenesis. Future Oncol. 1, 23-35. https://doi.org/10.1517/14796694.1.1.23
  4. Aruoma, O. I. 1994. Nutrition and health aspects of free radicals and antioxidants. Food Chem. Toxicol. 32, 671-683. https://doi.org/10.1016/0278-6915(94)90011-6
  5. Bang, K. S. 2008. Cytotoxic and antioxidative effects of Saururus chinensis. J. Cosm. Public Health 4, 220-225.
  6. Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26, 1199-1200.
  7. Borenfreund, E. and Puerner, J. A. 1984. A simple quantitative procedure using monolayer culture for cytotoxicity assay (HTD/NR-90). J. Tiss. Cult. Meth. 9, 7-9.
  8. Cho, H. Y., Cho, C. W. and Song, Y. S. 2005. Antioxidative and anti-inflammatory effects of saururus chinensis methanol extract in RAW 264.7 macrophages. J. Med. Food 8, 190-197. https://doi.org/10.1089/jmf.2005.8.190
  9. Davies, K. F. and Goldberg, A. L. 1987. Proteins damaged by oxygen radicals are rapidly degraded in extracts of red blood cells. J. Biol. Chem. 262, 8227-8261.
  10. Fubini, B. 1998. Surface chemistry and quartz hazard. Ann. Occup. Hyg. 42, 521-530.
  11. Halliwell, B. J. and Gutteridge, M. C. 1984. Oxygen toxicity, oxygen radicals, transition metals and disease. Eur. J. Biochem. 219, 1-14.
  12. Iurlaro, M., Benelli, R., Masiello, L., Rosso, M., Santi, L. and Albini, A. 1998. Beta interferon inhibits HIV-1 Tat-induced angiogenesis synergism with 13-cis retinoic acid. Eur. J. Cancer 34, 570-576. https://doi.org/10.1016/S0959-8049(97)10082-X
  13. Kameyama, K., Takemura, T., Hamada, Y., Sakai, C., Kondoh, S., Nishiyama, S., Urabe, K. and Hearing, V. J. 1993. Pigment production in murine melanoma cells is regulated by tyrosinase. tyrosinase-related protein 1 (TRP 1) dopachrome tautomerase (TRP 2) and a melanogenic inhibitor. J. Invest. Dermatol. 100, 126-131. https://doi.org/10.1111/1523-1747.ep12462778
  14. Kim, S. K., Ban, S. Y., Kim, J. S. and Chung, S. K. 2005. Change of antioxidant activity and antioxidant compounds in Saururus chinensis by extraction conditions. J. Korean Soc. Appl. Biol. Chem. 48, 89-92.
  15. Koh, M. S. 2004. Antimicrobial activity of Saururus chinensis Baill extract. J. Korean Soc. Food Sci. Nutr. 33, 1098-1105. https://doi.org/10.3746/jkfn.2004.33.7.1098
  16. Kumaran, A. and Karunakaran, R. J. 2006. Antioxidant and free radical scavenging activity of an aqueous extract of Coleus aromaticus. Food Chem. 97, 109-114. https://doi.org/10.1016/j.foodchem.2005.03.032
  17. Kwak, J. W. and Kwon, C. H. 1988. Pharmacological studies on Saururus chinensis Baill. Bull. KH Pharma. Sci. 16, 137-154.
  18. Kwon, H. J., Jung, U., Park, H. R., Shin, D. H. and Jo, S. K. 2007. Effects of gamma irradiation on color changes and antioxidative ctivities of Caesalpinia sappan L. J. Korean Soc. Food Sci. Nutr. 36, 1055-1061. https://doi.org/10.3746/jkfn.2007.36.8.1055
  19. Lee, J. T., Jung, S. H., Jo, W. A., Kang, B. Y., Choi, E. Y., Jung, Y. S. and Son, A. R. 2005. A study on the materials of functional korean herb cosmetics using atractylodis Rhizoma Alba and Saururi herba seu Radix. Korean J. Oriental Med. 5, 7-14.
  20. Lee, S. T., Park, J. M., Lee, H. K., Kim, M. B., Cho, J. S. and Heo, J. S. 2000. Component comparison in different growth stages and organs of Saururus chinensis Baill. Korean J. Medicinal Crop Sci. 8, 312-318.
  21. Lee, S. T., Lee, Y. H., Choi, Y. J., Lee, Y. H., Cho, J. S. and Heo, J. S. 2001. Yield and bioactive compponent on different compost amounts and cultural methods of Saururus chinensis Baill. Korean J. Medicinal Crop Sci. 9, 220-224.
  22. Maeura, Y., Weisburger, J. H. and Williams, G. 1984. Dose-dependent reduction of N-2-fluorenylacetamide-induced liver cancer and enhancement of bladder cancer in rats by butylated hydroxytoluene. Cancer Res. 44, 1604-1610.
  23. Nakamura, T., Lozano, P. R., Ikeda, Y., Iwanaga, Y., Hinek, A., Minamisawa, S., Cheng, C. F., Kobuke, K., Dalton, N., Takada, Y., Tashiro, K., Ross Jr, J., Honjo, T. and Chien, K. R. 2002. Fibulin-5/DANCE is essential for elastogenesis in vivo. Nature 415, 171-175. https://doi.org/10.1038/415171a
  24. Park, D. J. and Lee, J. C. 2008. A study on the antioxidative and depigmentation activities of the ethanol extract of Saururus herba. Korean J. Herbology 23, 193-202.
  25. Park, J. H. and Lee, J. G. 2000. The encyclopedia of medicinal plants. pp. 202-203, Shin il publishing Co., Seoul, Korea.
  26. Park, J. H., Park, B. G., Kim, M. J., Park, S. G. and Kim, J. H. 1998. Effects of tuber pasition and number of nodes on growth of Saururus chinensis Baill. Korean J. Medicinal Crop Sci. 6, 286-293.
  27. Park, S. J., Yoo, H. J., Choi, H. S., Seo, B. Y., Yang, S. H., Kim, Y. H., Jeong, J. Y. and Lee, K. N. 2004. Effect of Saururus chinensis Baill extracts on antibacterial activity and cadmium induced cytotoxicity. Korean J. Oriental Prev. Med. Society 8, 81-98.
  28. Park, S. K., Oh, G. J., Kim, H. T., Kim, H. J., Chung, S. G. and Cho, E. H. 1998. Analgesic constituent from the Herba of Saururus chinensis (Lour.) Baill. Yakhak Heoji 42, 238-242.
  29. Pontieri, V. and Sage, T. L. 1999. Evidence for stigmatic self incompatibility, pollination induced ovule enlargement and transmitting tissue exudates in the paleoherd, Saururus cernuus L. (Saururacese). Ann. Bot. 84, 507-519. https://doi.org/10.1006/anbo.1999.0947
  30. Rehman, Z., Habbib, F. and Shah, W. H. 2004. Utilization of potatopeels extract as a natural anti-oxidant in bean oil. Food Chem. 85, 215-220. https://doi.org/10.1016/j.foodchem.2003.06.015
  31. Seo, H. S., Chung, B. H. and Cho, Y. G. 2008. Antioxdant and anticancer effects of agrimony (Agrimonia pilosa L.) and chinese lizardtail (Saururus chinensis Baill). Korean J. Medicinal Crop Sci. 16, 139-143.
  32. Shin, H. H., Kang, M. J., Cho, H. Y., Kim, B. C. and Cho, E. K. 2008. Optimization of extraction conditions for Houttuynia cordara Thunb and Saururus chinensis Baill mixture by response surface methodology. Food Eng. Prog. 12, 247-255.
  33. Valko, M., Rhodes, C. J., Moncol, J., Izakovic, M. and Mazur, M. 2006. Free radicals, metals and antioxidants in oxidative stress induced cancer. Chem. Biol. Interact. 160, 1-40. https://doi.org/10.1016/j.cbi.2005.12.009
  34. Yanagisawa, H., Davis, E. C., Starcher, B. C., Ouchi, T., Yanagisawa, M., Richardson, J. A. and Olson, E. N. 2002. Fibulin-5 is an elastin-binding protein essential for elastic fiber development in vivo. Nature 415, 168-171. https://doi.org/10.1038/415168a
  35. Yoo, H. J., Kang, H. J., Jung, H. J., Kim, K. H., Chang, J. L. and Park, E. H. 2008. Anti-inflammatory, anti-angiogenic and anti-nociceptive activities of Saururus chinensis extract. J. Erthnopharmacol. 120, 282-286. https://doi.org/10.1016/j.jep.2008.08.016

Cited by

  1. Physicochemical properties of Salvia miltiorrhiza Bunge following treatment with enzymes vol.22, pp.5, 2015, https://doi.org/10.11002/kjfp.2015.22.5.699
  2. Effect of Anti-oxidant, Anti-inflammatory and Anti-invasive of PMA-induced Matrix Metalloproteinase (MMP-2) and MMP-9 Activities of Water Extract and Solvent Fractions of Saururus Chinensis vol.26, pp.5, 2016, https://doi.org/10.5352/JLS.2016.26.5.584