생명과학회지 (Journal of Life Science)

  • 제22권6호
  • /
  • Pages.799-806
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  • 2012
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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Bacillus sp. HJ 57에 의한 버섯 병원균주의 생육억제

Growth Inhibition of Mushroom Pathogen by Bacillus sp. HJ 57

  • 서권일 (순천대학교 식품영양학과) ;
  • 갈상완 (경남과학기술대학교 제약공학과) ;
  • 이성태 (순천대학교 생물학과) ;
  • 박경욱 (에스엔제이바이오(주)) ;
  • 이상원 (경남과학기술대학교 제약공학과)
  • Seo, Kwon-Il (Department of Food and Nutrition, Sunchon National University) ;
  • Gal, Sang-Won (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Yee, Sung-Tae (Department of Biology, Sunchon National University) ;
  • Park, Kyung-Wuk (S&J Bio Co., Ltd., 303 Business Incubator, Sunchon National University) ;
  • Lee, Sang-Won (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
  • 투고 : 2012.03.21
  • 심사 : 2012.04.06
  • 발행 : 2012.06.30
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초록

발효된 버섯배지 및 부엽토 등으로부터 80여 종의 균주를 1차 분리하여 CMCase, amylase 및 protease활성이 높고 버섯질병균주에 대한 항균활성이 높으면서 표고버섯 및 팽이버섯의 생육에는 전혀 영향을 미치지 않는 HJ-57 항균미생물을 최종 분리균주로 선정하였다. 최종 선정한 HJ-57 항균미생물을 동정한 결과 Bacillus sp. HJ-57로 밝혀졌으며, 배양을 위한 배지의 초기 pH는 pH 7, 배양온도는 $35^{\circ}C$가 최적인 것으로 나타났다. Bacillus sp. HJ-57균주는 12 hr 배양하였을 때는 약간의 항균활성을 나타내었지만, 배양 36~48 hr 배양하였을 때는 비교적 높은 항균활성을 나타내었다. Bacillus sp. HJ-57 항균미생물은 팽이버섯 재배농가에서 사용하는 콘코프 함유배지상에서도 버섯질병균주인 세균 및 곰팡이에 대하여 매우 높은 항균활성을 나타내었다.

Approximately 80 species of bacteria were isolated from the fermented mushroom first and the HJ-57 antibacterial micro-organism was selected to the final isolation bacteria. It has a high degree of CMCase, amylase, and protease activity as well as high antibacterial activity against mushroom pathogenic bacteria without affecting the growth and development of Flammulina velutipes and Lentinus edodes mushrooms. The finally selected HJ-57 antibacterial micro-organism was identified as Bacillus sp. HJ-57. The initial pH for culture was pH7 and its optimum culture temperature was $35^{\circ}C$. The antibacterial material produced by Bacillus sp. HJ-57 showed a little antibacterial activity even in the 12 hr of culture, but showed the highest antibacterial activity in the 36~48 hr of culture. The HJ-57 antibacterial micro-organism also showed a high antibacterial activity against mushroom pathogenic bacteria and molds in the corn cob contained culture medium is used in Flammulina velutipes cultivators.

키워드

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