Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effects of Black Garlic Supplementation and Exercise on TBARS, HSP 70 and COX-2 Expression after High-intensity Exercise

흑마늘 투여와 운동이 고강도 운동 후 TBARS, HSP 70 및 COX-2 발현에 미치는 영향

  • Baek, Yeong-Ho (Department of Physical Education, Pusan National University) ;
  • Lee, Sang-Ho (Department of Physical Education, Pusan National University) ;
  • Han, Min-Ho (Department of Biomaterial Control, Graduate School, Dong-Eui University) ;
  • Choi, Yung-Hyun (Department of Biomaterial Control, Graduate School, Dong-Eui University) ;
  • Kim, Seung-Hyun (Department of Physical Education, Pusan National University) ;
  • Kwak, Yi-Sub (Department of Physical Education, Dong-Eui University)
  • 백영호 (부산대학교 체육교육과) ;
  • 이상호 (부산대학교 체육교육과) ;
  • 한민호 (동의대학교 대학원 바이오물질제어학과) ;
  • 최영현 (동의대학교 대학원 바이오물질제어학과) ;
  • 김승현 (부산대학교 체육교육과) ;
  • 곽이섭 (동의대학교 체육학과)
  • Received : 2012.03.13
  • Accepted : 2012.05.07
  • Published : 2012.06.30
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Abstract

The purpose of this study was to investigate the effects of black garlic supplementation and exercise on TBARS, HSP 70, and COX-2 expression after high-intensity exercise in rats. Twenty-four male Sprague-Dawley rats (4 weeks) were used in this study. Experimental groups were a control group (A, n=6), black garlic supplementation group (B, n=6), exercise group (C, n=6), and black garlic supplementation with exercise group (D, n=6). High-intensity exercise was performed by treadmill running (every 10 min speed increased gradually; 15~24 m/min, $0^{\circ}$ grade) and 2.86 g/kg black garlic for 4 weeks. The findings of this study were as follows: TBARS levels were significantly lower in the D group compared to the other group and B and C groups were significantly lower in TBARS levels than in the A group. HSP 70 expression was lower in the B group compared to the other groups. COX-2 expression has showed a similar expression in all groups. Therefore, it is considered that black garlic intake and exercise may reduce oxidative stress.

실험동물은 생후 4주령 Sprague Dawley계 수컷 흰쥐 24마리로 흑마늘운동군(6마리), 운동군(6마리), 흑마늘군(6마리), 대조군(6마리)으로 총 24마리로 구분하여 운동은 총 4주간 트레드밀로 실시하였으며, 시료 채취 하기 바로 직전에 고강도 운동을 실시하였다. 흑마늘 섭취는 흑마늘진액을 구강 투여시켰으며, 연구결과 다음과 같다. 4주간의 흑마늘 투여와 유산소운동 후 고강도 운동을 실시하였을 때 TBARS 농도는 흑마늘운동군이 흑마늘군, 운동군과 대조군에 비해 유의하게 낮았다. 흑마늘군과 운동군은 대조군에 비해 유의하게 낮았다. HSP 70 발현은 흑마늘섭취군이 다른군에 비해 상대적으로 낮게 나타났다. COX-2 발현은 모든군에서 비슷한 발현을 보이고 있으며 규칙적인 운동과 흑마늘 섭취는 산화적 스트레스를 줄일 수 있을 것으로 기대된다.

Keywords

References

  1. Abramson, J. L. and Vaccarino, V. 2002. Relationship between physical activity and inflammation among apparently healthy middle aged and older adults. Arch. Int. Med. 162, 1286-1292. https://doi.org/10.1001/archinte.162.11.1286
  2. Bae, G. W. 2009. Effects of black garlic intake on exhaust variables in blood, immunoglobulin, liver function and antioxidants after the maximal exercise. Ph. D. Dissertation. Keimyung University.
  3. Ban, H. N. 2010. The Effects of Circuit Training and Black Garlic Intake on Health Related Fitness, Lipid Profiles, C-Reactive Protein and Bone Density in Middle-aged Women. MS Thesis. Pusan National University.
  4. Bedford, T. G., Tipton, C. M., Wilson, N. C., Oppliger, R. A. and Gisolfi, C. V. 1979. Maximum oxygen consumption of rats and its changes with various experimental procedures. J. Appl. Physiol. 47, 1278-1283.
  5. Burgio, M. R., Kim, C. J., Dow, C. C. and Koretz, J. F. 2000. Correlation between the Chaperone-like Activity and Aggregate Size of a-Crestallin With Increasing Temperature. Biochem. Biophys. Res. Commun. 268, 426-432. https://doi.org/10.1006/bbrc.1999.2036
  6. Dillson, S. A., Lowe, G. M., Billington, D. and Rahman, K. 2002. Dietary supplementation with aged garlic extract reduces plasma and urine concentrations of 8-iso-protaglandin F(2 alpha) in smoking and nonsmoking men and women. J. Nutr. 132, 163-170.
  7. Duda, G., Suliburska, J. and Pupek-Musialik, D. 2008. Effects of shorts-term garlic supplementation on lipid metabolism and antioxidant status in hypertensive adults. Pharmacol. Rep. 60, 163-170.
  8. Eom, W. S. 2004. The effect of 12 weeks aerobic exercise with different exercise intensity on lipid peroxidation (MDA) and antioxidant enzyme (SOD). Exer. Sci. 13, 335-350.
  9. Ferrucci, L., Cherubini, A., Bandinelli, S., Bartali, B., Corsi, A. and Lauretani. B. 2006. Relationship of plasma polyunsaturated fatty acids to circulating inflammatory markers. J. Clin. Endocrinol. Meta. 91, 436-446.
  10. Geffken, D. F., Cushman, M., Benett, M., Burke, G. L., Polak, J. F., Sakkinen, P. A. and Tracy, R. P. 2001. Association between physical activity and markers of inflammation in a healthy elderly population. Am. J. Epidemiol. 183-187.
  11. Hart, F. U. and Hayer-Hartl, M. 2002. Molecular chape rones in the cytosol: from nascent chain to folded protein. Science 295, 1852-1858. https://doi.org/10.1126/science.1068408
  12. Huang, M., Stolina, M., Sharma, S., Mao, J. T., Zhu, L. P., Miller, W., Wollman, J., Herschman, H. and Dubinett, S. M. 1998. Non-small cell lung cancer cyclooxygenase-2-dependent regulation of cytokine balance in lymphocytes and macrophage Up-regulation of interleukin 10 and down-regulation of interleukin 12 production. Cancer Res. 58, 1208-1216.
  13. Jenkins, R. R. 1993. Exercise, oxidative stress and antioxidants: a review. Int. J. Sports Nutr. 3, 356-375.
  14. Jun, J. K., Yoon, A. R., Lee. K. M. and Lee, W. L. 2006. The effect of acute exhaustive exercise and long-term endurance exercise training on the protein expression of Mn-SOD, HSP70 and PPAR-$\gamma$ in the heart of rats. Kor. J. Exer. Nutr. 13, 161-167.
  15. Kevin, C. K. 2002. Heat shock proteins: modifying factors in physiological stress responses and acquired thermotolerance, J. Appl. Physiol. 92, 2177-2186.
  16. Kim, H. T. 2005. The expression of regular exercise on COX-2 mRNA and protein in the diabetic rats renal. Kor. J. Phy. Edu. 44, 381-390.
  17. Kimura, N., Satoshi, T., Iuchi, Y., Abe, H., Totsukawa, K. and Fujii, J. 2010. Intrinsic oxidative stress causes either two-cell arrest or cell death depending on developmental stage of the embryos from SOD1-deficient mice. Mol. Hum. Reprod. 16, 441-451. https://doi.org/10.1093/molehr/gaq007
  18. Knowlton, A. 2000. An overview of the heat shock proteins, their regulation, and function. In: Heat Shock Proteins and the Cardiovascular System, A. Knowlton (Ed). Boston: Kluwer Academic Publishers, 1-24.
  19. Koh, T. J. and Escobedo, J. 2004. Cytoskeletal disruption and small heat shock protein translocation immediately after lengthening contractions. Am. J. Physiol. Cell Physiol. 286, c713-c722. https://doi.org/10.1152/ajpcell.00341.2003
  20. Komers, R., Lindsley, J. N., Oyama, T. T., Schutzer, W. E., Reed, J. F. and Mader, S. L. 2001. Immunohistochemical and functional correlations of renal cyclooxygenase-2 in experimental diabetes. J. Clin. Invest. 107, 889-898. https://doi.org/10.1172/JCI10228
  21. Krihnan, R. K., Evans, W. J. and Kirwan, J. P. 2003. Impaired substrate oxidation in healthy elderly men after eccentric exercise. J. Appl. Physiol. 94, 716-723. https://doi.org/10.1063/1.1576489
  22. Lee, G. P. and Lim, I. S. 2000. The changes of MDA, SOD, and catalase by overtraining. Kor. J. Phy. Edu. 39, 426-435.
  23. Lee, S. H, and Baek, Y. H. 2011. The effect of leeki during exhaustive exercise on lipid metabolism and antioxidant enzyme activities in Rats. Kor. J. Sports Sci. 20, 1439-1450.
  24. Lee, H. M., Seo, D. Y., Lee, S. H. and Baek, Y. H. 2010. Effects of exhaustive exercise and aged garlic extract supplementation on weight, adipose tissue mass, lipid profiles and oxidative stress in high fat diet induced obese rats. J. Life Sci. 20, 1889-1895. https://doi.org/10.5352/JLS.2010.20.12.1889
  25. Locke, M. and Tanguay, R. M. 1996. Increased HSP activation in muscles with a high constitutive HSP70 expression. Cell Stress Chaperones 1, 189-196. https://doi.org/10.1379/1466-1268(1996)001<0189:IHAIMW>2.3.CO;2
  26. Monzillio, L. U. and Christos, S. 2003. Effect of lifestyle modification on adipokine levels in obese subjects with insulin resistance. Obese Res. 11, 1048-1054. https://doi.org/10.1038/oby.2003.144
  27. Moreno F. J., Corzo-Martinez, M., Castillo, M. D. and Villamiel, M. 2006. Change in antioxidant activity of dehydrated onion and garlic during storage. Food Research International 39, 185-192.
  28. Morimoto R. I. 1993. Cell in stress: Transcriptional activation of heat shock genes. Science 259, 1409-1410. https://doi.org/10.1126/science.8451637
  29. Naoaki, M., Mitsuyasu, U., Naoki, K., Takeshi, N., Minoru, H. and Hidekatsu, T. 2006. Aged garlic extract ameliorates physical fatigue. Biol. Pharm. Bull. 29, 962-966. https://doi.org/10.1248/bpb.29.962
  30. Oishi, Y., Taniguchi, K., Matsumoto, H., Ishihara, A., Ohira, Y. and Roy, R. R. 2002. Muscle type-specific response of HSP60, HSP72, and HSC73 during recovery after elevation of muscle temperature. J. Appl. Physiol. 92, 1097-1103.
  31. Paulsen, P. G., Vissing, K., Kalhovde, J. M., Ugelstad, I., Bayer, M. L., Kadi, F., Schjerling, P., Hallen, J. and Raastad, T. 2007. Maximal eccentric exercise induces a rapid accumulation of small heat shock proteins on myofibrils and a delayed HSP70 response in humans. Am. J. Physiol. 293, 844-853. https://doi.org/10.1152/ajpregu.00677.2006
  32. Powers, S. K., Ji, L. L. and Leeuwenburgh, C. 1999. Exercise training-induced alterations in skeletal muscle antioxidant capacity: a brief review. Med. Sci. Sports Exer. 31, 987-997. https://doi.org/10.1097/00005768-199907000-00011
  33. Reddy, G. B., Das, K. P., Petrash, J. M. and Surewicz, W. K. 2000. Temperature -dependent chaperone activity and structural properties of human $\alpha A$-and $\alpha B$-crystallins. J. Biol. Chem. 18, 4565-4570.
  34. Sato, T., Nakajima, H., Fujio, K. and Mori, Y. 1997. Enhancement of prostaglandin E2 production by epidermal growth factor requires the coordinate activation of cytosolic phospholipase A2 and cyclooxygenase 2 in human squamous carcinoma A431 cells. Prostaglandins 53, 355-369.
  35. Schwartz, I. F., Hershkovitz, R., Iaina, A., Gnessin, E., Wollman, Y., Chenichowski, T., Blum, M., Levo, Y. and Schwartz, D. 2002. Garlic attenuates nitric oxide production in rat cardiac myocytes thought inhibition of inducible nitric oxide synthase and arginine transporter CAT-2 (cationic amino acid transporter-2). Clin. Sci. 102, 487-493. https://doi.org/10.1042/CS20010221
  36. Seo, Y. H. 2004. Effects of TaeKwonDo training on blood antioxidant enzymes and lipid peroxidation. Chosun University.
  37. Seong, N. J. 2008. Physicochemical composition and antioxidant activity of black garlic. Food Preserv. Proc. Industry 7, 45-53.
  38. Smolka, M. B., Zoppi, C. C., Alves, A. A., Silveira, L. R., Marangoni, S., Pereira-Da-Silva, L., Novello, J. C. and Nacedo, D. V. 2000. HSP72 as a complementary protection against oxidative stress induced by exercise in the soleus muscle of rats. Am. J. Physiol. Regul. Integr. Comp Physiol. 279, R1539-R1545.
  39. Thompson, C. E., Thompson, T. R., Alers, J. J. and Decedue, C. J. 1985. Response of HDL cholesterol, apoprotein A-I, and LCAT to exercise withdrawal. Atherosclerosis 54, 65-73. https://doi.org/10.1016/0021-9150(85)90154-6
  40. Vane, J. R., Bakhle, Y. S. and Botting, R. M. 1998. Cyclooxygenase 1 and 2. Amu. Rev. Pharmacol. Toxicol. 38, 97-120. https://doi.org/10.1146/annurev.pharmtox.38.1.97
  41. Vigue, C. A. L. P., Ames, B. N. and Brooks, G. A. 1993. Oxidant stress in human blood during exercise. Appl. Physiol. 75, 566-572.
  42. Welch, W. J. 1993. Heat shock proteins functioning as molecular chaperones: their roles in normal and stressed cells. Philos. Trans. R. Soc. Lond B. Biol. Sci. 339, 327-333. https://doi.org/10.1098/rstb.1993.0031

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