생명과학회지 (Journal of Life Science)

  • 제22권6호
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  • Pages.723-729
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  • 2012
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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환경시료에 존재하는 미생물 배양을 위한 filter plate micro trap의 개발

Filter Plate Micro Trap as a Device for in situ Cultivation for Environmental Microorganisms

  • Jung, Da-Woon (Department of Environmental Science, Kangwon National University) ;
  • Ahn, Tae-Seok (Department of Environmental Science, Kangwon National University)
  • 투고 : 2012.01.18
  • 심사 : 2012.06.04
  • 발행 : 2012.06.30
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초록

난배양성 미생물의 배양과 보다 다양한 미생물의 분리를 위하여 Filter plate microbial trap (FPMT)을 개발하였고, 이 장치를 사용하여 Greenland의 토양에서 세균을 분리하였다. FPMT는 배지와 하단부의 membrane filter(0.45 ${\mu}m$ pore size)로 구성되어 있다. 시료에 포함된 세균과 화학물질이 FPMT 내로 이동하고, 세균이 증식하는 원리이다. 그 결과, FPMT를 이용한 새로운 in situ 배양방법을 통해 통상적인 Petri dish 배양방법보다 더 많은 종과 기존의 방법으로는 배양할 수 없는 세균을 분리하였다. 이 장치는 앞으로 다양한 환경에서 난배양성 미생물을 분리하는데 유용함을 확인하였다.

Filter plate microbial trap (FPMT) was invented as an in situ cultivation device for the isolation of bacteria from natural environments. FPMT consists of a medium and membrane filters (0.45 ${\mu}m$ pore size) and microorganisms and compounds can be moved freely moved into the medium. This device was applied to two soil samples of Greenland. The microbial diversity of both soil samples by FPMT was higher than that by the conventional Petri dish-based method. Moreover, novel bacterial species were isolated by FPMT. The new FPMT is effective for in situ cultivation of natural samples and could be applicable to the isolation of uncultivable microorganism.

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