Effect of Water Extract of Cynanchi Wilfordii Radix in RANKL-induced Osteoclast Differentiation

백하수오(白何首烏) 물 추출물의 파골세포 분화에 미치는 영향

  • Ahn, Yong-Hwan (Department of Anatomy, Institute for Skeletal Disease & Institute for Environmental Science, Wonkwang Medical Science, College of Medicine) ;
  • Oh, Jae-Min (Department of Anatomy, Institute for Skeletal Disease & Institute for Environmental Science, Wonkwang Medical Science, College of Medicine) ;
  • Lee, Myeung-Su (Department of Internal Medicine, Division of Rheumatology & Institute of Wonkwang Medical Science, College of Medicine) ;
  • Jung, Jong-Hyuk (Department of Internal Medicine, Division of Rheumatology & Institute of Wonkwang Medical Science, College of Medicine) ;
  • Chae, Soo-Uk (Department of Orthopedic Surgery, Gunsan Medical Center) ;
  • Moon, Seo-Young (Division of Anesthesiology and Pain Medicine, Sanbon Medical Center) ;
  • Jeon, Byung-Hoon (Department of Pathology & Research Center of Traditional Korean Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Choi, Min-Kyu (Department of Anatomy, Institute for Skeletal Disease & Institute for Environmental Science, Wonkwang Medical Science, College of Medicine)
  • 안용환 (원광대학교 의과대학 해부학교실, 골격계질환연구소 및 환경과학연구소) ;
  • 오재민 (원광대학교 의과대학 해부학교실, 골격계질환연구소 및 환경과학연구소) ;
  • 이명수 (원광대학교 의과대학 류마티스내과학교실 및 원광의과학연구소) ;
  • 정종혁 (원광대학교 의과대학 류마티스내과학교실 및 원광의과학연구소) ;
  • 채수욱 (군산병원 정형외과) ;
  • 문서영 (산본병원 마취통증의학과) ;
  • 전병훈 (한의과대학 병리학교실 및 한국전통의학연구소) ;
  • 최민규 (원광대학교 의과대학 해부학교실, 골격계질환연구소 및 환경과학연구소)
  • Received : 2012.02.06
  • Accepted : 2012.03.19
  • Published : 2012.04.25

Abstract

Osteoporotic fracture became a serious social problem, which related with mortality and morbidity in old age population. Osteoclast which is responsible for bone resorption is originated from hematopoietic cell line and plays a key role osteoporotic bone loss. Cynanchum wilfordii (Asclepiadaceae) roots have been used in Korean folk medicine for the treatment of diabetes mellitus and aging progression. Also, recent studies have shown that the extract and fractions of Cynanchi Wilfordii Radix have various pharmacological actions including scavenging free radicals, enhancing immunity, reducing high serum cholesterol, and anti-tumor activity. However, the effect of extract of Cynanchi Wilfordii Radix in osteoclast differentiation had not been reported. Thus, we evaluated the effect of Cynanchi Wilfordii Radix on receptor activator of nuclear factor-${\kappa}B$ ligand (RANKL)-induced osteoclast differentiation. Through our study, we found that Cynanchi Wilfordii Radix significantly inhibited osteoclast differentiation induced by RANKL. Cynanchi Wilfordii Radix suppressed the activation of p38 pathway and $NF{\kappa}B$ in bone marrow macrophages (BMMs) treated with RANKL. Also, Cynanchi Wilfordii Radix significantly inhibited the mRNA expression of c-Fos, tartrate-resistant acid phosphatase (TRAP), osteoclast-associated receptor (OSCAR), nuclear factor of activated T cells (NFAT)c1 and cathepsin K in BMMs treated with RANKL. Particularly, Cynanchi Wilfordii Radix inhibited the protein expression of c-fos and NFATc1. Taken together, our results demonstrated that Cynanchi Wilfordii Radix may be useful treatment option of bone-related disease such as osteoporosis leads to fracture of bone and rheumatoid arthritis.

Keywords

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