Proteomic Changes by Acupuncture Stimulation at HT7 in the Hippocampus of Rat Pups

신문혈 자침이 어린 백서 해마의 단백질 발현에 미치는 영향

  • Bae, Chang-Hwan (Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University) ;
  • Kim, Dong-Soo (Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University) ;
  • Kim, Seung-Tae (Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University)
  • 배창환 (부산대학교 한의학전문대학원 경락구조의학부) ;
  • 김동수 (부산대학교 한의학전문대학원 경락구조의학부) ;
  • 김승태 (부산대학교 한의학전문대학원 경락구조의학부)
  • Received : 2012.05.14
  • Accepted : 2012.06.08
  • Published : 2012.06.27

Abstract

Objectives : Hippocampus, a region of temporal lobe, plays an important role in the pathogenic mechanisms of brain diseases such as Alzheimer's disease, depression and temporal lobe epilepsy. This research is designed to investigate hippocampal changes after acupuncture stimulation at Shinmun(HT7) using 2-dimensional gel electrophoresis(2-DE). Methods : On postnatal-day 15, rat pups were randomly devided into Normal(NOR) or HT7 group. All of Pups kept with their mothers for 7 days, but pups in HT7 group received acupuncture stimulation at HT7 daily. On postnatal-day 21, hippocampus of each rat pup was dissceted 30 minutes after last acupuncture stimulation and the protein expressions were investigated using 2-DE. Results : After acupuncture stimulation at HT7, expression of 20 proteins were significantly increased. Succinate semialdehyde dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase-like, transketolase, aconitate hydratase and phosphoglucomutase-1 were related to glucose methabolism. Eukaryotic initiation factor(eIF) 4A-II, eIF 4A-III, mitochondrial Tu translation elongation factor and chain A of crystal structure of the 70-Kda heat shock cognate protein involve in the protein synthesis in ribosome. Tubulin ${\beta}$-4 chain, tubulin T ${\beta}$-15 and tubulin ${\alpha}$-1B chain comprise cytoskeleton. Glutathione S-transferase(GST) ${\omega}$-1, GST P and GST Yb-3 can reduce oxidative stress. ${\beta}$-soluble N-ethylmaleimide-sensitive fusion protein attachment protein is required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus, glycerol-3-phosphate dehydrogenase plays a major role in lipid biosynthesis, creatine kinase U-type catalyses the conversion of creatine and consumes adenosine triphosphate to create phosphocreatine and adenosine diphosphate. Platelet-activating factor acetylhydrolase IB subunit alpha and voltage depedent anion-selective channel protein 2 were also increased. Conclusions : The results suggest that acupuncture stimulation at HT7 may enhance glucose and lipid metabolism, protein synthesis, cytoskeletal substance and anti-oxidative stress in hippocampus.

Keywords

References

  1. Lee JG, Kim EH, Lee JH. Effect of gentianae radix on neurogenesis and apoptosis in hippocampus of ethanol-induced newborn rats. J Orient Neuropsychatr. 2010 ; 21(2) : 29-44.
  2. Hampel H, Burger K, Teipel SJ, Bokde AL, Zetterberg H, Blennow K. Core candidate neurochemical and imaging biomarkers of Alzheimer's disease. Alzheimers Dement. 2008 ; 4(1) : 38-48. https://doi.org/10.1016/j.jalz.2007.08.006
  3. Chang BS, Lowenstein DH. Epilepsy. N Engl J Med. 2003 ; 349(13) : 1257-66. https://doi.org/10.1056/NEJMra022308
  4. Joels M. Functional actions of corticosteroids in the hippocampus. Eur J Pharmacol. 2008 ; 583(2-3) : 312-21. https://doi.org/10.1016/j.ejphar.2007.11.064
  5. Yim YK. Details of meridians & acupoints; a guide book for college students. Wonju : Uibang Publishing Co. 2010 : 424.
  6. Jeon HJ, Lee BH, Lee KM, Kim JS, Lee YK, Lee JH, et al. A synergy effect of combination of acupoints in cocaine take. J Korean Acupunct Mox Soc. 2010 ; 27(2) : 31-41.
  7. Chae YB, Lee JC, Park KM, Lee HJ, Kang OS, Kim SY, et al. Inhibitory effect of acupuncture at HT7 on the sympathetic activations to smoking-related visual cues during smoking cessation. Korean J Acupunct. 2009 ; 26(4) : 39-52.
  8. Jang JY, Cho SY, Kim SJ, Kim YS, Nam SS. The effect of laser acupuncture at HT7(Sinmun) for mental stress on short-term analysis of heart rate variability. J Korean Acupunct Mox Soc. 2010 ; 27(5) : 51-8.
  9. Park HJ, Lim S, Lee HS, Lee HJ, Yoo YM, Kim SA, et al. Acupuncture enhances cell proliferation in dentate gyrus of maternallyseparated rats. Neurosci Lett. 2002 ; 319(3) : 153-6. https://doi.org/10.1016/S0304-3940(01)02581-2
  10. Lim S, Ryu YH, Kim ST, Hong MS, Park HJ. Acupuncture increases neuropeptide Y expression in hippocampus of maternallyseparated rats. Neurosci Lett. 2003 ; 343(1) : 49-52. https://doi.org/10.1016/S0304-3940(03)00317-3
  11. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 ; 7(72) : 248-54.
  12. Oakley BR, Kirsch DR, Morris NR. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal Biochem. 1980 ; 105(2) : 361-3.
  13. Fernandenz J, Gharahdaghi F, Mische SM. Routine identification of proteins from sodium dodecyl sulfate-polyacrylamide gel electrophoresis( SDS-PAGE) gels or polyvinyl difluoride membranes using matrix assisted laser desorption/ ionization-time of flight-mass spectrometry (MALDI-TOF-MS). Eletrophoresis. 1998 ; 19 : 1036-45. https://doi.org/10.1002/elps.1150190619
  14. Turner AJ, Whittle SR. Biochemical dissection of the $\gamma$-aminobutyrate synapse. Biochem J. 1983;209:29-41.
  15. Errington AC, Gibson KM, Crunelli V, Cope DW. Aberrant GABAA receptor-mediated inhibition in cortico-thalamic networks of succinic semialdehyde dehydrogenase deficient mice. PLoS ONE. 2011 ; 6(4) : e19021. https://doi.org/10.1371/journal.pone.0019021
  16. Sirover MA. Subcellular dynamics of multifunctional protein regulation: mechanisms of GAPDH intracellular translocation. J Cell Biochem. 2012 (Epub ahead of print) ; doi :10.1002/jcb.24113.
  17. Jaglin XH, Poirier K, Saillour Y, Buhler E, Tian G, Bahi-Buisson N, et al. Mutations in the beta-tubulin gene TUBB2B result in asymmetrical polymicrogyria. Nat Genet. 2009 ; 41(6) : 746-52. https://doi.org/10.1038/ng.380
  18. Kim ST, Park HJ, Hong MS, Kim SN, Doo AR, Yin C, et al. Acupuncture stimulation to HT8 enhances cell proliferation in hippocampus on an epilepsy mouse model. Kor J Acupunct. 2010 ; 27(2) : 49-56.
  19. Sun KH, Chang KH, Clawson S, Ghosh S, Mirzaei H, Regnier F, et al. Glutathione- S-transferase P1 is a critical regulator of Cdk5 kinase activity. J Neurochem. 2011 ; 118(5) : 902-14. https://doi.org/10.1111/j.1471-4159.2011.07343.x
  20. Kim K, Kim SH, Kim J, Kim H, Yim J. Glutathione s-transferase omega 1 activity is sufficient to suppress neurodegeneration in a Drosophila model of Parkinson disease. J Biol Chem. 2012 ; 287(9) : 6628-41. https://doi.org/10.1074/jbc.M111.291179
  21. Wallimann T, Wyss M, Brdiczka D, Nicolay K, Eppenberger HM. Intracellular compartmentation, structure and function of creatine kinase isoenzymes in tissues with high and fluctuating energy demands: the 'phosphocreatine circuit' for cellular energy homeostasis. Biochem J. 1992 ; 281(1) : 21-40.
  22. Pozzi D, Condliffe S, Bozzi Y, Chikhladze M, Grumelli C, Proux-Gillardeaux V, et al. Activity- dependent phosphorylation of Ser187 is required for SNAP-25-negative modulation of neuronal voltage-gated calcium channels. PNAS. 2008 ; 105(1) : 323-8. https://doi.org/10.1073/pnas.0706211105
  23. Godbole A, Mitra R, Dubey AK, Reddy PS, Mathew MK. Bacterial expression, purification and characterization of a rice voltage-dependent, anion-selective channel isoform, OsVDAC4. J Membr Biol. 2011 ; 244(2) : 67-80. https://doi.org/10.1007/s00232-011-9399-x
  24. Subedi KP, Kim JC, Kang M, Son MJ, Kim YS, Woo SH. Voltage-dependent anion channel 2 modulates resting $Ca^{2+}$ sparks, but not action potential-induced $Ca^{2+}$ signaling in cardiac myocytes. Cell Calcium. 2011 ; 49(2) : 136-43. https://doi.org/10.1016/j.ceca.2010.12.004
  25. Nguyen NHT, Brathe A, Hassel B. Neuronal uptake and metabolism of glycerol and the neuronal expression of mitochondrial glycerol-3-phosphate dehydrogenase. J Neurochem. 2003 ; 85(4) : 831-42. https://doi.org/10.1046/j.1471-4159.2003.01762.x
  26. Nishida K, Markey SP. Platelet-activating factor in brain regions after transient ischemia in Gerbils. Stroke. 1996 ; 27 : 514-9. https://doi.org/10.1161/01.STR.27.3.514
  27. Jeon S, Kim YJ, Kim ST, Moon W, Chae Y, Kang M, et al. Proteomic analysis of the neuroprotective mechanism of acupuncture treatment in a Parkinson's disease mouse model. Proteomics. 2008 ; 8(22) : 4822-32. https://doi.org/10.1002/pmic.200700955
  28. Kim HJ, Park HJ, Hong MS, Song JY, Park HY, Jo DJ, et al. Effect by acupuncture on hypothalamic expression of maternally separated rats: proteomic approach. Neurol Res. 2010 ; 32(Suppl 1) : S69-73.
  29. Kim ST, Jeon S, Park HJ, Hong MS, Jeong WB, Kim JH, et al. Acupuncture inhibits kainic-acid induced hippocampal cell death in mice. J Physiol Sci. 2008 ; 58(1) : 31-8. https://doi.org/10.2170/physiolsci.RP010907