Journal of Environmental Impact Assessment (환경영향평가)

Korean Society of Environmental Impact Assessment (한국환경영향평가학회)
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Sampling and Cryogenic Pulverization and Storage of Environmental Samples and Improvement of Operating Procedures in National Environmental Specimen Bank

국가환경시료은행 시료 채취, 분쇄, 저장과 개선방안 고찰

  • Lee, Jangho (Ecosystem Assessment Division, Environmental Resources Research Department, National Institute of Environmental Research) ;
  • Lee, Jongchun (Ecosystem Assessment Division, Environmental Resources Research Department, National Institute of Environmental Research) ;
  • Kim, Myungjin (Nature Conservation Research Division, Environmental Resources Research Department, National Institute of Environmental Research) ;
  • Han, Areum (Ecosystem Assessment Division, Environmental Resources Research Department, National Institute of Environmental Research) ;
  • Lee, Eugene (Ecosystem Assessment Division, Environmental Resources Research Department, National Institute of Environmental Research) ;
  • Bade, Rabindra (Ecosystem Assessment Division, Environmental Resources Research Department, National Institute of Environmental Research) ;
  • Kim, Minsung (Ecosystem Assessment Division, Environmental Resources Research Department, National Institute of Environmental Research)
  • 이장호 (국립환경과학원 환경자원연구부 자연평가연구팀) ;
  • 이종천 (국립환경과학원 환경자원연구부 자연평가연구팀) ;
  • 김명진 (국립환경과학원 환경자원연구부 자연자원연구과) ;
  • 한아름 (국립환경과학원 환경자원연구부 자연평가연구팀) ;
  • 이유진 (국립환경과학원 환경자원연구부 자연평가연구팀) ;
  • 바데 라빈드라 (국립환경과학원 환경자원연구부 자연평가연구팀) ;
  • 김민성 (국립환경과학원 환경자원연구부 자연평가연구팀)
  • Received : 2012.09.11
  • Accepted : 2012.11.16
  • Published : 2012.12.31
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Abstract

Environmental Specimen Banks (ESBs) are playing pivotal role in monitoring the effect of environmental pollution on the ecosystem based on the retrospective analysis of the representative samples collected regularly and stored in cryogenic condition. In Korea, National Environmental Specimen Bank (NESB) was established in 2009 and the standard operating procedures (SOPs) for sampling, and cryogenic milling and storage had been prepared during 2007-2010. Since then, the tentative SOPs for the seven kinds of specimens (shoots of Red Pine (Pinus densiflora) and Korean Pine (Pinus koraiensis), leaves of Mongolian Oak (Quercus mongolica) and Zelkova Tree (Zelkova serrata), eggs of Feral Pigeon (Columba livia var. domestica), muscles and organs of Common Carp (Cyprinus carpio), and Freshwater Bivalve (Unio (Nodularia) douglasiae)) have been put to test in the field and laboratory as well against the practicality and feasibility. The SOPs were improved by reflecting the findings from the research and the following discussion regarding the selection of specimen (Feral Pigeon suffering from a control management), sample size (a problem of decreasing number of sampling trees related to increasing sampling time) and period (a problem related to a bud growth), and sampling methods etc.. In addition, barcoding system for the management of the specimen information, and monitoring system of the cryogenic storage to regulate the optimum temperature and the liquid nitrogen level were also developed for the efficient and effective control of the samples. Lastly, the safety guide and emergency protocol were augmented to guarantee a safe work environment with the cryogenic facility. These improvements of the SOPs are expected to contribute to more stable operation of the NESB.

Keywords

References

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