Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Expression and Characterization of ${\alpha}$-Methylacyl CoA Racemase from Anisakis simplex Larvae

  • Kim, Bong-Jin (Department of Internal Medicine, Kosin University College of Medicine) ;
  • Kim, Sun-Mi (Department of Pharmacology, Pusan National University School of Medicine) ;
  • Cho, Min-Kyung (Department of Parasitology, Pusan National University School of Medicine) ;
  • Yu, Hak-Sun (Department of Parasitology, Pusan National University School of Medicine) ;
  • Lee, Yong-Seok (Department of Parasitology, Inje University College of Medicine) ;
  • Cha, Hee-Jae (Department of Parasitology and Genetics, Kosin University College of Medicine) ;
  • Ock, Mee-Sun (Department of Parasitology and Genetics, Kosin University College of Medicine)
  • Received : 2011.10.25
  • Accepted : 2012.02.22
  • Published : 2012.06.15
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Abstract

Larval excretory-secretory products of Anisakis simplex are known to cause allergic reactions in humans. A cDNA library of A. simplex 3rd-stage larvae (L3) was immunoscreened with polyclonal rabbit serum raised against A. simplex L3 excretory-secretory products to identify an antigen that elicits the immune response. One cDNA clone, designated as ${\alpha}$-methylacyl CoA racemase (Amacr) contained a 1,412 bp cDNA transcript with a single open reading frame that encoded 418 amino acids. A. simplex Amacr showed a high degree of homology compared to Amacr orthologs from other species. Amacr mRNA was highly and constitutively expressed regardless of temperature (10-$40^{\circ}C$) and time (24-48 hr). Immunohistochemical analysis revealed that Amacr was expressed mainly in the ventriculus of A. simplex larvae. The Amacr protein produced in large quantities from the ventriculus is probably responsible for many functions in the development and growth of A. simplex larvae.

Keywords

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