Parasites, Hosts and Diseases

  • 제50권2호
  • /
  • Pages.137-141
  • /
  • 2012
  • /
  • 2982-5164(pISSN)
  • /
  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
권호 표지
DOI QR코드

DOI QR Code

Dirofilaria repens in Vietnam: Detection of 10 Eye and Subcutaneous Tissue Infection Cases Identified by Morphology and Molecular Methods

  • De, Nguyen Van (Department of Parasitology, Hanoi Medical University) ;
  • Le, Thanh Hoa (Department of Immunology, Institute of Bio-Technology) ;
  • Chai, Jong-Yil (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine)
  • 투고 : 2011.12.22
  • 심사 : 2012.03.15
  • 발행 : 2012.06.15
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

From 2006 to 2010, hospitals in Hanoi treated 10 human patients for dirofilariasis. The worms were collected from parasitic places, and identification of the species was completed by morphology and molecular methods. Ten parasites were recovered either from the conjunctiva (n=9) or subcutaneous tissue (n=1). The parasites were 4.0-12.5 cm in length and 0.5-0.6 mm in width. Morphological observations suggested all parasites as Dirofilaria repens. Three of the 10 parasites (1 from subcutaneous tissue and 2 from eyes) were used for molecular confirmation of the species identification. A portion of the mitochondrial cox1 (461 bp) was amplified and sequenced. Nucleotide and amino acid homologies were 95% and 99-100%, respectively, when compared with D. repens (Italian origin, GenBank AJ271614; DQ358814). This is the first report of eye dirofilariasis and the second report of subcutaneous tissue dirofilariasis due to D. repens in Vietnam.

키워드

참고문헌

  1. Pampiglione S, Rivasi F. Human dirofilariasis due to Dirofilaria (Nochtiella) repens: An update of world literature from 1995 to 2000. Parasitologia 2000; 42: 231-254.
  2. Cancrini,G, Scaramozzino P, Gabrielli S, Di Paolo M, Toma L, Romi R. Aedes albopictus and Culex pipiens implicated as natural vectors of Dirofilaria repens in central Italy. J Med Entomol 2007; 44: 1064-1066.
  3. Canestri TG, Pampiglione S, Rivasi F. The species of the genus Dirofilaria Railliet & Henry, 1911. Parasitology 1997; 39: 369-374.
  4. Hoa LV, Ty LT. Comparative study of Dirofilaria macacae, Sandground 1933, a parasite of primates, and Dirofilaria repens, Raillet and Henry 1911, a parasite of Vietnamese carnivora. Bull Soc Pathol Exot Filiales 1971; 64: 347-360.
  5. Dujic MP, Mitrovic BS, Zec IM. Orbital swelling as a sign of live Dirofilaria repens in subconjunctival tissue. Scand J Infect Dis 2003; 35: 430-431.
  6. Raniel Y, Machamudov Z, Garzozi HJ. Subconjunctival infection with Dirofilaria repens. Isr Med Assoc J 2006; 8: 139.
  7. Rishniw M, Barr SC, Simpson KW, Frongillo MF, Franz M, Dominguez-Alpizar JL. Discrimination between six species of canine microfilariae by a single polymerase chain reaction. Vet Parasitol 2006; 135: 303-314.
  8. Casiraghi M, Anderson TJ, Bandi C, Bazzocchi C, Genchi C. A phylogenetic analysis of filarial nematodes: Comparison with the phylogeny of Wolbachia endosymbionts. Parasitology 2011; 122: 93-103.
  9. Hu M, Gasser RB. Mitochondrial genomes of parasitic nematodes-progress and perspectives. Trends Parasitol 2006; 22: 78-84.
  10. Le TH, Blair D, McManus DP. Mitochondrial genomes of parasitic flatworms. Trends Parasitol 2002; 18: 206-213.
  11. Miyazaki I. An Illustrated Book of Helminthic Zoonoses. International Medical Foundation of Japan, Tokyo, Japan. Southeast Asian Medical Information Center. 1991, p 422-436.
  12. De NV, Le TH, Chau HTM, Huan LQ. Human dirofilariasis in the world and the first identification for species in Vietnam. J Pharmaceut Med 2008; 5: 11-15 (in Vietnamese with English abstract).
  13. Dang TCT, Nguyen TH, Dung DT, Uga S, Morishima Y, Sugiyama H, Yamasaki H. A human case of subcutaneous dirofilariasis caused by Dirofilaria repens in Vietnam: Histologic and molecular confirmation. Parasitol Res 2010; 107: 1003-1007.
  14. Hu M, Gasser RB, Abs El-Osta YG, Chilton NB. Structure and organization of the mitochondrial genome of the canine heartworm, Dirofilaria immitis. Parasitology 2003; 127: 37-51.
  15. Ghedin E, Wang S, Spiro D, Caler E, Zhao Q, Crabtree J, Allen JE, Delcher AL, Guiliano DB, Miranda-Saavedra D, Angiuoli SV, Creasy T, Amedeo P, Haas B, El-Sayed NM, Wortman JR, Feldblyum T, Tallon L, Schatz M, Shumway M, Koo H, Salzberg SL, Schobel S, Pertea M, Pop M, White O, Barton GJ, Carlow CK, Crawford MJ, Daub J, Dimmic MW, Estes CF, Foster JM, Ganatra M, Gregory WF, Johnson NM, Jin J, Komuniecki R, Korf I, Kumar S, Laney S, Li BW, Li W, Lindblom TH, Lustigman S, Ma D, Maina CV, Martin DM, McCarter JP, McReynolds L, Mitreva M, Nutman TB, Parkinson J, Peregrín-Alvarez JM, Poole C, Ren Q, Saunders L, Sluder AE, Smith K, Stanke M, Unnasch TR, Ware J, Wei AD, Weil G, Williams DJ, Zhang Y, Williams SA, Fraser-Liggett C, Slatko B, Blaxter ML, Scott AL. Draft genome of the filarial nematode parasite Brugia malayi. Science 2007; 317: 1756-1760.
  16. Keddie EM, Higazi T, Unnasch TR. The mitochondrial genome of Onchocerca volvulus: sequence, structure and phylogenetic analysis. Mol Biochem Parasitol 1998; 95: 111-127.
  17. Tamura K, Nei M, Kumar S. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci USA 2007; 101: 11030-11035.

피인용 문헌

  1. The First Human Case of Thelazia callipaeda Infection in Vietnam vol.50, pp.3, 2012, https://doi.org/10.3347/kjp.2012.50.3.221
  2. A Rare Human Case of Dirofilaria repens Infection in the Subcutaneous Posterior Thorax with Molecular Identification vol.53, pp.3, 2012, https://doi.org/10.3347/kjp.2015.53.3.329
  3. First Molecular Identification of Dirofilaria repens in a Dog Blood Sample from Guanajuato, Mexico vol.16, pp.11, 2012, https://doi.org/10.1089/vbz.2016.1948
  4. Molecular characterization of human Dirofilaria isolates from Kerala vol.146, pp.4, 2012, https://doi.org/10.4103/ijmr.ijmr_1425_15
  5. High genetic diversity in the Dirofilaria repens species complex revealed by mitochondrial genomes of feline microfilaria samples from Narathiwat, Thailand vol.66, pp.1, 2019, https://doi.org/10.1111/tbed.13033