Parasites, Hosts and Diseases

  • 제50권2호
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  • Pages.103-111
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  • 2012
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Molecular Identification and Real-time Quantitative PCR (qPCR) for Rapid Detection of Thelohanellus kitauei, a Myxozoan Parasite Causing Intestinal Giant Cystic Disease in the Israel Carp

  • Seo, Jung-Soo (Pathology Division, National Fisheries Research and Development Institute (NFRDI)) ;
  • Jeon, Eun-Ji (Pathology Division, National Fisheries Research and Development Institute (NFRDI)) ;
  • Kim, Moo-Sang (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Woo, Sung-Ho (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Kim, Jin-Do (Pathology Division, National Fisheries Research and Development Institute (NFRDI)) ;
  • Jung, Sung-Hee (Pathology Division, National Fisheries Research and Development Institute (NFRDI)) ;
  • Park, Myoung-Ae (Pathology Division, National Fisheries Research and Development Institute (NFRDI)) ;
  • Jee, Bo-Young (Aquatic Life Disease Control Division, NFRDI) ;
  • Kim, Jin-Woo (Aquatic Life Disease Control Division, NFRDI) ;
  • Kim, Yi-Cheong (New Strategy Research Center, NFRDI) ;
  • Lee, Eun-Hye (Pathology Division, National Fisheries Research and Development Institute (NFRDI))
  • 투고 : 2011.11.23
  • 심사 : 2012.01.23
  • 발행 : 2012.06.15
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초록

Intestinal giant-cystic disease (IGCD) of the Israel carp (Cyprinus carpio nudus) has been recognized as one of the most serious diseases afflicting inland farmed fish in the Republic of Korea, and Thelohanellus kitauei has been identified as the causative agent of the disease. Until now, studies concerning IGCD caused by T. kitauei in the Israel carp have been limited to morphological and histopathological examinations. However, these types of diagnostic examinations are relatively time-consuming, and the infection frequently cannot be detected in its early stages. In this study, we cloned the full-length 18S rRNA gene of T. kitauei isolated from diseased Israel carps, and carried out molecular identification by comparing the sequence with those of other myxosporeans. Moreover, conventional PCR and real-time quantitative PCR (qPCR) using oligonucleotide primers for the amplification of 18S rRNA gene fragment were established for further use as methods for rapid diagnosis of IGCD. Our results demonstrated that both the conventional PCR and real-time quantitative PCR systems applied herein are effective for rapid detection of T. kitauei spores in fish tissues and environmental water.

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참고문헌

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  2. New phylogenomic and comparative analyses provide corroborating evidence that Myxozoa is Cnidaria vol.81, pp.None, 2014, https://doi.org/10.1016/j.ympev.2014.08.016
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  4. A new species Myxodavisia jejuensis n. sp. (Myxosporea: Sinuolineidae) isolated from cultured olive flounder Paralichthys olivaceus in South Korea vol.118, pp.11, 2012, https://doi.org/10.1007/s00436-019-06454-z
  5. Molecular phylogenetics reveals a species complex pattern of closely related members of genus Thelohanellus (Cnidaria: Myxosporea) from the Indian subcontinent vol.150, pp.None, 2012, https://doi.org/10.1016/j.micpath.2020.104690