International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
권호 표지

Effect of Mineral Trioxide Aggregate and Calcium Hydroxide on Reparative Dentin Formation in Rats

  • Ra, Ji-Young (Department of Pediatric Dentistry, School of Dentistry, Wonkwang University) ;
  • Lee, Wan (Department of Oral & Maxillofacial Radiology, School of Dentistry, Wonkwang University) ;
  • Kim, Hyun-Jin (Institute of Biomaterial.Implant, Department of Oral Anatomy, School of Dentistry, Wonkwang University)
  • Received : 2012.05.23
  • Accepted : 2012.06.19
  • Published : 2012.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

We investigated the pulpal response to direct pulp capping in rat molar teeth using mineral trioxide aggregate (MTA) and calcium hydroxide (CH). A palatal cavity was prepared in rat maxillary molar teeth. Either MTA or CH was placed on the exposed pulp and all cavities were restored with composite. Rats were sacrificed for histological evaluation after 12 hours and at 2, 7, 14 and 21 days. In both the MTA and CH groups, reparative dentin formation was clearly observed on histology after 14 days. The MTA-capped pulps were found to be mostly free from inflammation, and hard tissue of a tubular consistent barrier was observed. In contrast, in CH-capped teeth, excessive formation of reparative dentin toward residual pulp was evident. The pulpal cell response beneath the reparative dentin layer was examined by immunofluorescence using antibodies against DSP. After 2 days, a few DSP immunopositive cells, most of which showed a cuboidal shape, appeared beneath the predentin layer. At 7 days, DSP-immunopositive cells with columnar odontoblast-like cells were seen beneath the newly formed hard tissues. At 14 and 21 days, DSP was more abundant in the vicinity of the odontoblastic process along the dentinal tubules than in the mineralized reparative dentin. The CH group showed strong expression patterns in terms of DSP immunoreactivity. Our results thus indicate that MTA may be a more effective pulp capping material as it induces the differentiation of odontoblast-like cells and the formation of reparative dentin without the loss of residual pulp functions.

Keywords

References

  1. Pfaff P, quoted by Glass RL, Zander HA. Pulp healing. J Dent Res 1949;28:97-107.
  2. Hermann BW. Dentinobliteran der Wurzelkanalc nach der Behandlung mit Kalzium, Zahnarztl Rundschau. 1930;39:888.
  3. Holland R, de Souza V, de Mello W, et al. Permeability of the hard tissue bridge formed after pulpotomy with calcium hydroxide. A histological study. J Am Dent Assoc. 1979;99:472-5.
  4. Schroeder U. Effects of calcium-hydroxide-containing pulp-capping agents on pulp cell migration, proliferation and differentiation. J Dent Res 1985;64:541-8.
  5. Kim JH, Hong JB, Lim BS, Cho BH. Histological evaluation of direct pulp capping with DSP-derived systhetic peptide in beagle dog. J Kor Acad Cons Dent 2009;34:120-9.
  6. Goldberg M, Six N, Decup F, Buch D, Soheili Majd C, Lasfargues JJ, Salih E, Stanislawski L. Application of bioactive molecules in pulp-capping situations. Adv Dent Res. 2001;15:91-5.
  7. Silva LA, Freitas AC, Carvalho FK, Queiroz AM, Nelson-Filho P, Porto-Neto ST. Direct pulp capping with a selfetching adhesive system: histopathologic evaluation in dogs' teeth. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009;108:34-40.
  8. Ford TR, Torabinejad M, Abedi HR, Bakland LK, Kariyawasam SP. Using mineral trioxide aggregate as a pulp-capping material. J Am Dent Assoc. 1996;127:1491-4.
  9. Junn DJ. Quantitative assessment of dentin bridge formation following pulp-capping with mineral trioxide aggregate [master's thesis]. Loma Linda, CA: Loma Linda University, 2000.
  10. Faraco IM Jr, Holland R. Response of the pulp of dogs to capping with mineral trioxide aggregate or a calcium hydroxide cement. Dent Traumatol. 2001;17:163-6.
  11. Nyborg H. Healing process in the pulp after capping. Acta Odontol Scand. 1955;13:1-30.
  12. Masterton JB. The healing of wounds of the dental pulp. Dent Pract Dent Rec 1966;16:325-39.
  13. Kozlov M, Massler M. Histologic effect of various drugs on amputated pulps of rat molars. Oral Surg 1960;13:455-69.
  14. Butler WT. Sialoproteins of bone and dentin. J Biol Buccale. 1991;19:83-9.
  15. Butler WT. Dentin matrix proteins and dentinogenesis. Connect Tissue Res. 1995;33:59-65.
  16. Yamamura T. Differentiation of pulpal cells and inductive influences of various matrices with reference to pulpal wound healing. J Dent Res. 1985;64:530-40.
  17. Tziafas D. Mechanisms controlling secondary initiation of dentinogenesis: a review. Int Endod J. 1994;27:61-74.
  18. Goldberg M, Smith AJ. Cells and extracellular matrices of dentin and pulp: a biological basis for repair and tissue engineering. Crit Rev Oral Biol Med. 2004;15:13-27.
  19. Barthel CR, Rosenkranz B, Leuenberg A, Roulet JF. Pulp capping of carious exposures: treatment outcome after 5 and 10 years: a retrospective study. J Endod. 2000;26:525-8.
  20. Lee SJ, Monsef M, and Torabinejad M. Sealing ability of a mineral trioxide aggregate for repair of lateral root perforations. J Endod. 1993;19:541-44.
  21. Witherspoon DE. Vital pulp therapy with new materials: new directions and treatment perspectives permanent teeth. J Endod. 2008; 34:25-28.
  22. Olsson H, Petersson K, Rohlin M. Formation of a hard tissue barrier after pulp cappings in humans. A systematic review. Int Endod J. 2006;39:429-42.
  23. Prasad M, Butler WT, Qin C. Dentin sialophosphoprotein in biomineralization. Connect Tissue Res. 2010;89:808-12.
  24. Min KS, Park HJ, Lee SK, Park SH, Hong CU, Kim HW, Lee HH, Kim EC. Effect of mineral trioxide aggregate on dentin bridge formation and expression of dentin sialoprotein and heme oxygenase-1 in human dental pulp. J Endod. 2008;34:666-70.