Bulletin of the Korean Chemical Society

  • 제33권7호
  • /
  • Pages.2163-2167
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  • 2012
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  • 0253-2964(pISSN)
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  • 1229-5949(eISSN)
대한화학회 (Korean Chemical Society)
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Quantitative Determination of Amitriptyline and Its Metabolite in Rat Plasma by Liquid Chromatography-tandem Mass Spectrometry

  • Chae, Jung-Woo (College of Pharmacy, Chungnam National University) ;
  • Baek, In-Hwan (Incrementally Modified Drug (IMD), Yuhan Research Institute) ;
  • An, Jung-Hwa (College of Pharmacy, Chungnam National University) ;
  • Kim, Eun-Jung (Division of Pharmacological Research, National Institute of Food and Drug Evaluation, Korea Food & Drug Evaluation) ;
  • Kwon, Kwang-Il (College of Pharmacy, Chungnam National University)
  • 투고 : 2012.02.21
  • 심사 : 2012.03.26
  • 발행 : 2012.07.20
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초록

A rapid, specific, and reliable LC-MS/MS-based bioanalytical method was developed and validated in rat plasma for the simultaneous quantitation of amitriptyline and its metabolite nortriptyline. Chromatographic separation of these analytes was achieved on a Gemini C18 column ($50{\times}4.60mm$, $5{\mu}m$) using reversed-phase chromatography. The mobile phase was an isocratic solvent system consisting of 1% formic acid in water and methanol (10:90, v/v), at a flow rate of 0.2 mL/min. The analytical range was set as 0.1-500 ng/mL for amitriptyline and 0.08-500 ng/mL for nortriptyline using a $200{\mu}L$ plasma sample. The accuracy and precision of the assay were in accordance with FDA regulations for the validation of bioanalytical methods. The validated method was successfully applied to a pharmacokinetic study in six rats after oral administration of amitriptyline (15 mg/kg). This method allows laboratory scientists to rapidly determine amitriptyline and nortriptyline concentrations in plasma.

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참고문헌

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  3. Genetic Polymorphisms on the Pharmacokinetics of Amitriptyline and Its Metabolite, Nortriptyline pp.00912700, 2018, https://doi.org/10.1002/jcph.1344
  4. A Study on CYP2C19 and CYP2D6 Polymorphic Effects on Pharmacokinetics and Pharmacodynamics of Amitriptyline in Healthy Koreans vol.10, pp.2, 2012, https://doi.org/10.1111/cts.12451
  5. Preparation and characterization of nano amitriptyline hydrochloride particles by spray freeze drying vol.14, pp.12, 2012, https://doi.org/10.2217/nnm-2018-0116
  6. Paradigm Shift in the Arena of Sample Preparation and Bioanalytical Approaches Involving Liquid Chromatography Mass Spectroscopic Technique vol.35, pp.10, 2012, https://doi.org/10.2116/analsci.19r003
  7. Practical liquid chromatography–tandem mass spectrometry method for the simultaneous quantification of amitriptyline, nortriptyline and their hydroxy metabolites in human serum vol.33, pp.12, 2012, https://doi.org/10.1002/bmc.4679