Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Quantitative Determination of Amitriptyline and Its Metabolite in Rat Plasma by Liquid Chromatography-tandem Mass Spectrometry

  • Chae, Jung-Woo (College of Pharmacy, Chungnam National University) ;
  • Baek, In-Hwan (Incrementally Modified Drug (IMD), Yuhan Research Institute) ;
  • An, Jung-Hwa (College of Pharmacy, Chungnam National University) ;
  • Kim, Eun-Jung (Division of Pharmacological Research, National Institute of Food and Drug Evaluation, Korea Food & Drug Evaluation) ;
  • Kwon, Kwang-Il (College of Pharmacy, Chungnam National University)
  • Received : 2012.02.21
  • Accepted : 2012.03.26
  • Published : 2012.07.20
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Abstract

A rapid, specific, and reliable LC-MS/MS-based bioanalytical method was developed and validated in rat plasma for the simultaneous quantitation of amitriptyline and its metabolite nortriptyline. Chromatographic separation of these analytes was achieved on a Gemini C18 column ($50{\times}4.60mm$, $5{\mu}m$) using reversed-phase chromatography. The mobile phase was an isocratic solvent system consisting of 1% formic acid in water and methanol (10:90, v/v), at a flow rate of 0.2 mL/min. The analytical range was set as 0.1-500 ng/mL for amitriptyline and 0.08-500 ng/mL for nortriptyline using a $200{\mu}L$ plasma sample. The accuracy and precision of the assay were in accordance with FDA regulations for the validation of bioanalytical methods. The validated method was successfully applied to a pharmacokinetic study in six rats after oral administration of amitriptyline (15 mg/kg). This method allows laboratory scientists to rapidly determine amitriptyline and nortriptyline concentrations in plasma.

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References

  1. Krishnan, K.; Greenblatt, D. J.; Von Moltke, L. L.; Schmider, J.; Harmatz, J. S.; Shader, R. I. J. Clin. Pharmacol. 1998, 38, 112. https://doi.org/10.1002/j.1552-4604.1998.tb04399.x
  2. Ghahramani, P.; Lennard, M. S. J. Chromatogr. B: Biomed. Appl. 1996, 685, 307. https://doi.org/10.1016/S0378-4347(96)00193-4
  3. Aymard, G.; Livi, P.; Pham, Y. T.; Diquet, B. J. Chromatogr. B: Biomed. Sci. Appl. 1997, 700, 183. https://doi.org/10.1016/S0378-4347(97)00327-7
  4. Olesen, O. V.; Plougmann, P.; Linnet, K. J. Chromatogr. B: Biomed. Sci. Appl. 2000, 746, 233. https://doi.org/10.1016/S0378-4347(00)00332-7
  5. Kudo, K.; Jitsufuchi, N.; Imamura, T. J. Anal. Toxicol. 1997, 21, 185. https://doi.org/10.1093/jat/21.3.185
  6. Kollroser, M.; Schober, C. Ther. Drug. Monit. 2002, 24, 537. https://doi.org/10.1097/00007691-200208000-00013
  7. Shen, Y.; Zhu, R. H.; Li, H. D.; Liu, Y. W.; Xu, P. J. Pharm. Biomed. Anal. 2010, 53, 735. https://doi.org/10.1016/j.jpba.2010.04.031
  8. Bhatt, M.; Shah, S.; Shivprakash. Biomed. Chromatogr. 2010, 24, 1247. https://doi.org/10.1002/bmc.1435
  9. Schaaf, L. J.; Dobbs, B. R.; Edwards, I. R.; Perrier, D. G. Eur. J. Clin. Pharmacol. 1988, 34, 533. https://doi.org/10.1007/BF01046718
  10. Schaaf, L. J.; Dobbs, B. R.; Edwards, I. R.; Perrier, D. G. Eur. J. Clin. Pharmacol. 1988, 34, 439. https://doi.org/10.1007/BF01046699
  11. Matuszewski, B. K.; Constanzer, M. L.; Chavez-Eng, C. M. Anal. Chem. 2003, 75, 3019. https://doi.org/10.1021/ac020361s

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