Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Age-dependent Distribution of Fungal Endophytes in Panax ginseng Roots Cultivated in Korea

  • Park, Young-Hwan (School of Biotechnology, Yeungnam University) ;
  • Kim, Young-Chang (Department of Herbal and Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Park, Sang-Un (Department of Crop Science, Chungnam National University) ;
  • Lim, Hyoun-Sub (Department of Applied Biology, Chungnam National University) ;
  • Kim, Joon-Bum (Warm-Temperate Forest Research Center, Korea Forest Research Institute) ;
  • Cho, Byoung-Kwan (Department of Biosystems and Machinery Engineering, Chungnam National University) ;
  • Bae, Han-Hong (School of Biotechnology, Yeungnam University)
  • Received : 2012.01.17
  • Accepted : 2012.05.01
  • Published : 2012.07.15
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Abstract

Fungal endophytes were isolated from 1-, 2-, 3-, and 4-year-old ginseng roots (Panax ginseng Meyer) cultivated in Korea. The isolated fungal endophytes were identified based on sequence analysis of the internal transcribed spacer and morphological characterization by microscopic observations. A total of 81 fungal endophytes were isolated from 24 ginseng roots. Fungal endophytes were classified into 9 different fungal species and 2 unknown species. Ginseng roots that were 1-, 2-, 3-, and 4-years old were colonized by 2, 6, 8, and 5 species of fungal endophytes, respectively. While Phoma radicina was the most frequent fungal endophyte in 2-, 3-, and 4-year-old ginseng roots, Fusarium solani was the dominant endophyte in 1-year-old ginseng roots. The colonization frequencies (CF) varied with the host age. The CF were 12%, 40%, 31%, and 40% for 1-, 2-, 3-, and 4-year-old ginseng roots, respectively. We found a variety of fungal endophytes that were distributed depending on the age of ginseng plants.

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References

  1. Aly AH, Debbab A, Proksch P. Fungal endophytes: unique plant inhabitants with great promises. Appl Microbiol Biotechnol 2011;90:1829-1845. https://doi.org/10.1007/s00253-011-3270-y
  2. Schardl CL, Leuchtmann A, Spiering MJ. Symbioses of grasses with seedborne fungal endophytes. Annu Rev Plant Biol 2004;55:315-340. https://doi.org/10.1146/annurev.arplant.55.031903.141735
  3. Tan RX, Zou WX. Endophytes: a rich source of functional metabolites. Nat Prod Rep 2001;18:448-459. https://doi.org/10.1039/b100918o
  4. Rodriguez RJ, White JF Jr, Arnold AE, Redman RS. Fungal endophytes: diversity and functional roles. New Phytol 2009;182:314-330. https://doi.org/10.1111/j.1469-8137.2009.02773.x
  5. Strobel G. Harnessing endophytes for industrial microbiology. Curr Opin Microbiol 2006;9:240-244. https://doi.org/10.1016/j.mib.2006.04.001
  6. Bony S, Pichon N, Ravel C, Durix A, Balfourier F, Guillaumin JJ. The relationship between mycotoxin synthesis and isolate morphology in fungal endophytes of Lolium perenne. New Phytol 2001;152:125-137. https://doi.org/10.1046/j.0028-646x.2001.00231.x
  7. Schulz B, Boyle C, Draeger S, Rommert AK, Krohn K. Endophytic fungi: a source of novel biologically active secondary metabolites. Mycol Res 2002;106:996-1004. https://doi.org/10.1017/S0953756202006342
  8. Choi KT. Botanical characteristics, pharmacological effects and medicinal components of Korean Panax ginseng C A Meyer. Acta Pharmacol Sin 2008;29:1109-1118. https://doi.org/10.1111/j.1745-7254.2008.00869.x
  9. Shim M, Lee YJ. Ginseng as a complementary and alternative medicine for postmenopausal symptoms. J Ginseng Res 2009;33:89-92. https://doi.org/10.5142/JGR.2009.33.2.089
  10. Kim MH, Hong HD, Kim YC, Rhee YK, Kim KT, Rho J. Ginsenoside changes in red ginseng manufactured by acid impregnation treatment. J Ginseng Res 2010;34:93-97. https://doi.org/10.5142/jgr.2010.34.2.093
  11. He CN, Gao WW, Yang JX, Bi W, Zhang XS, Zhao YJ. Identification of autotoxic compounds from fi brous roots of Panax quinquefolium L. Plant Soil 2009;318:63-72. https://doi.org/10.1007/s11104-008-9817-8
  12. Leung KW, Wong AS. Pharmacology of ginsenosides: a literature review. Chin Med 2010;5:20. https://doi.org/10.1186/1749-8546-5-20
  13. Cho NS, Kim DH, Cho HY, Shin YS, Kim YC, Ohga S. Identification of symbiotic arbuscular mycorrhizal fungi in Korean ginseng roots by 18S rDNA sequence. J Fac Agric Kyushu Univ 2007;52:265-274.
  14. Li TS. Evaluation of chemical and non-chemical treatments for the control of ginseng replant disease. Acta Hortic (ISHS) 1994;363:141-146.
  15. Rural Development Administration. Ginseng GAP standard cultivation guideline. Suwon: National Institute of Crop Science, 2009.
  16. White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal genes for phylogenetics, In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, eds. PCR protocols: a guide to methods and applications. San Diego: Academic Press, 1990. p.315-322.
  17. Park SU, Lim HS, Park KC, Park YH, Bae HH. Fungal endophytes from three cultivars of Panax ginseng Meyer cultivated in Korea. J Ginseng Res 2012;36:107-113. https://doi.org/10.5142/jgr.2012.36.1.107
  18. Quilliam RS, Jones DL. Fungal root endophytes of the carnivorous plant Drosera rotundifolia. Mycorrhiza 2010;20:341-348. https://doi.org/10.1007/s00572-009-0288-4
  19. Dreyfuss MM, Chapela IH. Potential of fungi in the discovery of novel, low molecular weight pharmaceuticals. In: Gullo VP, ed. The discovery of natural products with therapeutic potential. Boston: Butterworth-Heinemann, 1994. p.49-80.
  20. Rodriguez R, Redman R. More than 400 million years of evolution and some plants still can't make it on their own: plant stress tolerance via fungal symbiosis. J Exp Bot 2008;59:1109-1114. https://doi.org/10.1093/jxb/erm342
  21. Xing X, Guo S, Fu J. Biodiversity and distribution of endophytic fungi associated with Panax quinquefolium L. cultivated in a forest reserve. Symbiosis 2010;51:161-166. https://doi.org/10.1007/s13199-010-0062-6
  22. Fravel D, Olivain C, Alabouvette C. Fusarium oxysporum and its biocontrol. New Phytol 2003;157:493-502. https://doi.org/10.1046/j.1469-8137.2003.00700.x
  23. Zhao YJ, Wang YP, Shao D, Yang JS, Liu D. Autotoxicity of Panax quinquefolium L. Allelophath J 2005;15:67-74.
  24. Seghers D, Wittebolle L, Top EM, Verstraete W, Siciliano SD. Impact of agricultural practices on the Zea mays L. endophytic community. Appl Environ Microbiol 2004;70:1475-1482. https://doi.org/10.1128/AEM.70.3.1475-1482.2004
  25. Marin S, Companys E, Sanchis V, Ramos AJ, Magan N. Effect of water activity and temperature on competing abilities of common maize fungi. Mycol Res 1998;102:959-964. https://doi.org/10.1017/S0953756297005613
  26. Niemeyer HM, Perez FJ. Potential of hydroxamic acids in the control of cereal pests, diseases and weeds. In: Inderjit, Daksini KM, Einhellig FA, eds. Allelopathy:organisms, processes, and applications. Washington, DC:American Chemical Society, 1995. p.260-270.
  27. VanEtten H, Temporini E, Wasmann C. Phytoalexin (and phytoanticipin) tolerance as a virulence trait: why is it not required by all pathogens? Physiol Mol Plant Pathol 2001;59:83-93. https://doi.org/10.1006/pmpp.2001.0350
  28. Carter JP, Spink J, Cannon PF, Daniels MJ, Osbourn AE. Isolation, characterization, and avenacin sensitivity of a diverse collection of cereal-root-colonizing fungi. Appl Environ Microbiol 1999;65:3364-3372.
  29. Arnold AE, Mejia LC, Kyllo D, Rojas EI, Maynard Z, Robbins N, Herre EA. Fungal endophytes limit pathogen damage in a tropical tree. Proc Natl Acad Sci U S A 2003;100:15649-15654. https://doi.org/10.1073/pnas.2533483100
  30. Nicol RW, Yousef L, Traquair JA, Bernards MA. Ginsenosides stimulate the growth of soilborne pathogens of American ginseng. Phytochemistry 2003;64:257-264. https://doi.org/10.1016/S0031-9422(03)00271-1
  31. Saunders M, Kohn LM. Host-synthesized secondary compounds influence the in vitro interactions between fungal endophytes of maize. Appl Environ Microbiol 2008;74:136-142. https://doi.org/10.1128/AEM.01538-07
  32. Stinson KA, Campbell SA, Powell JR, Wolfe BE, Callaway RM, Thelen GC, Hallett SG, Prati D, Klironomos JN. Invasive plant suppresses the growth of native tree seedlings by disrupting belowground mutualisms. PLoS Biol 2006;4:e140. https://doi.org/10.1371/journal.pbio.0040140
  33. Broeckling CD, Broz AK, Bergelson J, Manter DK, Vivanco JM. Root exudates regulate soil fungal community composition and diversity. Appl Environ Microbiol 2008;74:738-744. https://doi.org/10.1128/AEM.02188-07
  34. Aveskamp MM, De Gruyter J, Crous PW. Biology and recent developments in the systematics of Phoma, a complex genus of major quarantine significance. Fungal Divers 2008;31:1-18.
  35. Maddau L, Cabras A, Franceschini A, Linaldeddu BT, Crobu S, Roggio T, Pagnozzi D. Occurrence and characterization of peptaibols from Trichoderma citrinoviride, an endophytic fungus of cork oak, using electrospray ionization quadrupole time-of-flight mass spectrometry. Microbiology 2009;155(Pt 10):3371-3381. https://doi.org/10.1099/mic.0.030916-0
  36. Schulz B, Boyle C. The endophytic continuum. Mycol Res 2005;109(Pt 6):661-686. https://doi.org/10.1017/S095375620500273X
  37. Wang LW, Xu BG, Wang JY, Su ZZ, Lin FC, Zhang CL, Kubicek CP. Bioactive metabolites from Phoma species, an endophytic fungus from the Chinese medicinal plant Arisaema erubescens. Appl Microbiol Biotechnol 2012;93:1231-1239. https://doi.org/10.1007/s00253-011-3472-3
  38. Zhou X, Zhu H, Liu L, Lin J, Tang K. A review: recent advances and future prospects of taxol-producing endophytic fungi. Appl Microbiol Biotechnol 2010;86:1707-1717. https://doi.org/10.1007/s00253-010-2546-y

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