Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Diagnostic Assay of Toxic Zinc in an Ex Vivo Cell Using Voltammetry

  • Ly, Suw-Young (Biosensor Research Institute, Seoul National University of Science and Technology) ;
  • Yoo, Hai-Soo (Korea Ocean R&D Institute)
  • Received : 2012.06.13
  • Accepted : 2012.06.29
  • Published : 2012.06.30
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Abstract

Voltammetric detection of the toxic Zn ion was investigated using a fluorine-doped graphite pencil electrode (FPE). It is notable from the study that pencils were used as reference and working electrodes. In all the experiments, a clean seawater electrolyte solution was used to yield good results. The analytical working range was attained to 10 ${\mu}gL^{-1}$. The optimized voltammetric condition was examined to maximize the effect of the detection of trace Zn. The developed sensor was applied to an earthworm's tissue cell. It was found that the methods can be applicable to in vivo fluid or agriculture soil and plant science.

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References

  1. Ayoub, A.S., McGaw, B.A. and Midwood, A.J. (2002). Determination of Cd and Zn by isotope dilution-thermal ionisation mass spectrometry using a sequential analysis procedure. Talanta., 57, 405-413. https://doi.org/10.1016/S0039-9140(02)00019-X
  2. Azubel, M., Fernandez, F.M., Tudino, M.B. and Troccoli, O.E. (1999). Novel application and comparison of multivariate calibration for the simultaneous determination of Cu, Zn and Mn at trace levels using flow injection diode array spectrophotometry. Analytica Chimica Acta, 398, 93-102. https://doi.org/10.1016/S0003-2670(99)00375-X
  3. Bianchi, F., Maffini, M., Mangia, A., Marengo, E. and Mucchino, C. (2007). Experimental design optimization for the ICP-AES determination of Li, Na, K, Al, Fe, Mn and Zn in human serum. J. Pharm. Biomed. Anal., 43, 659-665. https://doi.org/10.1016/j.jpba.2006.07.054
  4. Capitan-Vallvey, L.F., Titos, A., Checa, R. and Navas, N. (2002). High-performance liquid chromatography determination of Znbacitracin in animal feed by post-column derivatization and fluorescence detection. J. Chromatogr. A, 943, 227-234. https://doi.org/10.1016/S0021-9673(01)01442-X
  5. Evers, T.H., Appelhof, M.A., de Graaf-Heuvelmans, P.T., Meijer, E.W. and Merkx, M. (2007). Ratiometric Detection of Zn(II) Using Chelating Fluorescent Protein Chimeras. J. Mol. Biol. 374, 411-425. https://doi.org/10.1016/j.jmb.2007.09.021
  6. Fabris, C., Soncin, M., Miotto, G., Fantetti, L., Chiti, G., Dei, D., Roncucci, G. and Jori, G. (2006). Zn(II)-phthalocyanines as phototherapeutic agents for cutaneous diseases. Photosensitization of fibroblasts and keratinocytes. J. Photochem. Photobiol. B, 83, 48-54. https://doi.org/10.1016/j.jphotobiol.2005.12.006
  7. Horng, C.J. and Lin, S.R. (1997). Determination of urinary trace elements (As, Hg, Zn, Pb, Se) in patients with Blackfoot dis-ease. Talanta., 45, 75-83. https://doi.org/10.1016/S0039-9140(97)00111-2
  8. Kefala, G., Economou, A., Voulgaropoulos, A. and Sofoniou, M. (2003). A study of bismuth-film electrodes for the detection of trace metals by anodic stripping voltammetry and their application to the determination of Pb and Zn in tapwater and human hair. Talanta., 61, 603-610. https://doi.org/10.1016/S0039-9140(03)00350-3
  9. Kohler, M., Harms, A.V. and Alber, D. (2000). Determination of Zn in high-purity GaAs with neutron activation analysis. Appl. Radiat. Isot., 53, 197-201. https://doi.org/10.1016/S0969-8043(00)00133-0
  10. Locatelli, C. and Torsi, G. (2000). Determination of Se, As, Cu, Pb, Cd, Zn and Mn by anodic and cathodic stripping voltammetry in marine environmental matrices in the presence of reciprocal interference. Proposal of a new analytical procedure. Microchemical Journal, 65, 293-303 https://doi.org/10.1016/S0026-265X(00)00128-4
  11. Lu, H.H. and Jiang, S.J. (2001). Organic acids as the modifier to determine Zn, Cd, Tl and Pb in soil by slurry sampling electrothermal vaporization inductively-coupled plasma mass spectrometry. Analytica Chimica Acta, 429, 247-255. https://doi.org/10.1016/S0003-2670(00)01288-5
  12. Marcó, L.M., Jiménez, E., Hernández, E.A., Rojas, A. and Greaves, E.D. (2001). Determination of ZnCu ratio and oligoelements in serum samples by total reflection X-ray fluorescence spectrometry for cancer diagnosis. Spectrochimica Acta Part B, 56, 2195-2201. https://doi.org/10.1016/S0584-8547(01)00289-0
  13. Miller, L.M., Wang, Q., Telivala, T.P., Smith, R.J., Lanzirotti, A. and Miklossy, J. (2006). Synchrotron-based infrared and X-ray imaging shows focalized accumulation of Cu and Zn co-localized with b-amyloid deposits in Alzheimer's disease. J. Struct. Biol. 155, 30-37. https://doi.org/10.1016/j.jsb.2005.09.004
  14. Nascentes, C.C., Arruda, M.A., Nogueira, A.R. and Nóbrega, J.A. (2004). Direct determination of Cu and Zn in fruit juices and bovine milk by thermospray flame furnace atomic absorption spectrometry. Talanta., 64, 912-917. https://doi.org/10.1016/j.talanta.2004.04.004
  15. Nedeltcheva, T., Atanassova, M., Dimitrov, J. and Stanislavova, L. (2005). Determination of mobile form contents of Zn, Cd, Pb and Cu in soil extracts by combined stripping voltammetry. Analytica Chimica Acta, 528, 143-146. https://doi.org/10.1016/j.aca.2004.10.036
  16. Pohi, P. and Prusisz, B. (2007). Determination of Ca, Mg, Fe and Zn partitioning in UHT cow milks by two-column ion exchange and flame atomic absorption spectrometry detection. Talanta., 71, 715-721. https://doi.org/10.1016/j.talanta.2006.05.030
  17. Santon, A., Irato, P., Medici, V., D'lncà, R., Albergoni, V. and Sturniolo, G.C. (2003). Effect and possible role of Zn treatment in LEC rats, an animal model of Wilson's disease. Biochim. Biophys. Acta, 1637, 91-97. https://doi.org/10.1016/S0925-4439(02)00218-1
  18. Wu, Y., Li, N.B. and Luo, H.Q. (2008). Simultaneous measurement of Pb, Cd and Zn using differential pulse anodic stripping voltammetry at a bismuth/poly(p-aminobenzene sulfonic acid) film electrode. Sensors and Actuators B, 133, 677-681. https://doi.org/10.1016/j.snb.2008.04.001
  19. Zaporozhets, O., Petruniock, N., Bessarabova, O. and Sukhan, V. (1999). Determination of Cu(II) and Zn(II) using silica gel loaded with 1-(2-thiasolylazo)-2-naphthol. Talanta., 49, 899- 906. https://doi.org/10.1016/S0039-9140(99)00085-5
  20. Zareba, S., Szarwil⁄o, K. and Pomykalski, A. (2005). Determination of Fe(II) and Zn(II) by spectrophotometry, atomic absorption spectrometry and ions chromatography methods in Vitrum. Farmaco., 60, 459-464. https://doi.org/10.1016/j.farmac.2004.03.014
  21. Zheng, F. and Hu, B. (2007). Preparation of a high pH-resistant AAPTS-silica coating and its application to capillary microextraction (CME) of Cu, Zn, Ni, Hg and Cd from biological samples followed by on-line ICP-MS detection. Anal. Chim. Acta, 605, 1-10. https://doi.org/10.1016/j.aca.2007.10.018