Ocean and Polar Research

Korea Institute of Ocean Science & Technology (한국해양과학기술원)
권호 표지
DOI QR코드

DOI QR Code

Effects of Starvation on the Morphometric Characteristics and Histological Changes in Chum Salmon (Oncorhynchus keta) Fry

  • Seong, Ki-Baik (Inland Aquaculture Research Center, NFRDI) ;
  • Park, In-Seok (Division of Marine Environment and Bioscience, College of Ocean Science and Technology Korea Maritime University) ;
  • Goo, In-Bon (Division of Marine Environment and Bioscience, College of Ocean Science and Technology Korea Maritime University) ;
  • Kim, Dong-Soo (Institute of Marine Living Modified Organisms (iMLMO), Pukyoung National University)
  • Received : 2012.01.13
  • Accepted : 2012.06.03
  • Published : 2012.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

A 26 day experiment was conducted to determine the effects of feeding and starvation on the survival, morphology, and histology in chum salmon (Oncorhynchus keta) fry. We included three experimental groups: starved, fed, and initial. The survival and growth rates were lower in the starved group than in the fed group (P < 0.05). In the starved group, survival began to decline after 16 days, and all fish had died after 26 days. We determined the effects of starvation on the morphometric parameters using the truss and classical dimensions. The dimensions in the head region were larger in the starved group than in the initial and fed groups. In contrast, the truss dimensions of the fed group were larger than those of the initial and starved groups. Starvation reduced the heights of the hepatocyte nuclei and of the intestinal epithelium (P < 0.05). The starved group also showed atrophy of the digestive structures and shrinkage of the foregut and midgut. Starvation led to the degeneration and atrophy of the exocrine pancreas, in which the lumen was markedly diminished and the folds of mucosa were less apparent. The hepatocyte morphology in the starved group was abnormal compared with that of the initial and fed groups, with highly compact, irregularly shrunken nuclei. Melanomacrophages were randomly distributed in the kidneys of the starved group, and their abundance increased rapidly during the experiment. In contrast, neither the initial nor fed group had any melanomacrophages. These results suggest that the nutritional parameters used in this study are useful indices of nutritional status in chum salmon.

Keywords

References

  1. Agius C (1979) The role of melano-macrophage centres in iron storage in normal and diseased fish. J Fish Dis 2:337-343 https://doi.org/10.1111/j.1365-2761.1979.tb00175.x
  2. Agius C, Roberts RJ (1981) Effects of starvation on the melano-macrophage centres of fish. J Fish Biol 19:161-169 https://doi.org/10.1111/j.1095-8649.1981.tb05820.x
  3. Beamish RJ Bouillon DR (1993) Pacific salmon production trans in relation to climate. Can J Fish Aquat Sci 50: 2270-2291 https://doi.org/10.1139/f93-252
  4. Crespo S, Marin de Mateo M, Santamaria CA (2001) Histopathological observations during larval rearing of common dentex Dentex dentex L. (Sparidae). Aquaculture 192:121-132 https://doi.org/10.1016/S0044-8486(00)00459-2
  5. Currens KP, Sharpe CS, Hjort R, Schreek CB, Li HW (1989) Effect of different feeding regimes on the morphometrics of chinook salmon (Oncorhynchus tshawytscha) and rainbow trout (O. mykiss). Copeia 3:689-695
  6. Ehrlich KF, Blaxter JHS, Pemberton R (1976) Morphological and histological changes during the growth and starvation of herring and plaice larvae. Mar Biol 35:105-118 https://doi.org/10.1007/BF00390932
  7. Hunter JR (1981) Feeding ecology and predation of marine fish larvae. In: Lasker R (ed) Marine Fish Larvae. Seattle Wasington Grant Program, pp 34-77
  8. Hur JW, Jo JH, Park I-S (2006a) Effects of long-term starvation on hepatocyte ultrastructure of olive flounder, Paralichthys olivaceus. Ichthyol Res 53:306-310 https://doi.org/10.1007/s10228-006-0348-0
  9. Hur JW, Woo SR, Jo JH, Park I-S (2006b) Effects of starvation on kidney melano-macrophage centre in olive flounder, Paralichthys olivaceus (Temminck and Schlegel). Aquaculture 37:821-825 https://doi.org/10.1111/j.1365-2109.2006.01498.x
  10. Kostomarova AA (1962) Effect of starvation on the development of the larvae of bony fishes. Trudy Inst Morf Zhivot 40:4-77
  11. Larsson A, Lewander K (1973) Metabolic effects of starvation in the European eel, Anguilla anguilla L. Comp Biochem Physiol 44:367-374 https://doi.org/10.1016/0300-9629(73)90489-1
  12. Love RM (1980) The chemical biology of fishes. vol 2. Academic Press, London and New York, pp 50-75
  13. Mizuno S, Misaka N, Miyakoshi Y, Takeuchi K, Kasahara N (2002) Effects of starvation on melano-macrophages in the kidney of masu salmon, Oncorhynchus masou. Aquaculture 209:247-255 https://doi.org/10.1016/S0044-8486(01)00716-5
  14. Myoung JG, Park CW, Kim MS, Kim JM, Kwang CB, Kim YU (1997) The fish fauna of Munsom in Chehu-do, Korea. Korean J Ichthyol 9:15-21
  15. Park I-S (2004) Effect of starvation on some parameter in Rhynchocypris oxycephalus (Saubage and Dabry): A review. Korean J Environ Biol 22:351-368
  16. Park I-S (2006) Histological changes of hepatocyte and intestinal epithelium during starvation in olive flounder Paralichthys olivaceus. J Korean Fish Soc 39:303-307
  17. Park I-S, Im JH, Ryu DK, Nam YK, Kim DS (2001) Effect of starvation on morphometric changes in Rhynchocypris oxycephalus (Sauvage and Darbry). J Appl Ichthyol 17: 277-281 https://doi.org/10.1046/j.1439-0426.2001.00298.x
  18. Park I-S, Johnson SC (2002) Determination of the temperature dependent index of mitotic interval for chromosome manipulation in winter flounder Pseudopleuronectes americanus. Aquaculture 213:95-100 https://doi.org/10.1016/S0044-8486(01)00886-9
  19. Park I-S, Kim JM, Kim YH, Kim DS (1988) Influence of lidocaine as an anaesthetic for marine fishes. J Fish Pathol 1:123-130
  20. Park I-S, Lee C-K, Im JH, Kim JH, Kim SU (1998) Effect of starvation on the growth and hepatocyte unclear size of larval rockfish Sebastes schlegeli and larval spotted sea bass Lateolabrax sp. J Aquacult 11:345-352
  21. Park I-S, Woo SR, Song YC, Cho SH (2007) Effect of starvation on morphometric characteristics of olive flounder, Paralichthys olivaceus. Ichthyol Res 54:297-302 https://doi.org/10.1007/s10228-007-0404-4
  22. Robert JB (2002) Trout and salmon of north America. A chanticleer press edition, The free press, New York, 51 p
  23. Robertson OH, Hane S, Wexler BC, Rinfret AP (1963) The effect of hydrocortisone on immature rainbow trout (Salmo gairdneri). Gen Comp Endocrinol 3:422-436 https://doi.org/10.1016/0016-6480(63)90056-X
  24. Storch V, Stählin W, Juario JV (1983) Effect of different diets on the ultrastructure of hepatocytes of Chanos chanos fry (Chanidae : Teleostei) and electron microscopic and morphometric analysis. Mar Biol 74:101-104 https://doi.org/10.1007/BF00394281
  25. Strauss RE, Bond CE (1990) Taxonomic methods, morphology. In: Schreck CE, Moyle PB (eds) Methods for Fish Biology. Amer Fish Soc, Bethesda, Maryland, pp 125-130
  26. Strauss RE, Bookstein FL (1982) The truss: Body from reconstruction in morphometrics. Syst Zool 31:113-135 https://doi.org/10.2307/2413032
  27. Theilacker GH (1978) Effect of starvation in the histological and morphological characteristics of jack mackerel, Trachurus symmetricus, larvae. Fish Bull 76:403-414
  28. Theilacker GH (1986) Starvation induced mortality of young sea-caught jack mackerel, Trachurus symmetricus, determined with histological and morphological methods. Fish Bull 84:1-17
  29. Weatherley AH, Gill HS (1987) The biology of fish growth. 4. Protein, lipid and caloric contents. Academic Press, London, 443 p

Cited by

  1. Induced Morphological Changes in Larval Rock Bream, Oplegnathus fasciatus, under Starvation vol.17, pp.4, 2013, https://doi.org/10.12717/DR.2013.17.4.399