Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Combined effects of temperature and arsenic on hematological parameters of tilapia Oreochromis niloticus

틸라피아의 혈액성상에 미치는 온도와 비소의 복합적 영향

  • Jeong, Ji-Won (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Kim, Young-Sug (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Kang, Ju-Chan (Department of Aquatic Life Medicine, Pukyong National University)
  • 정지원 (부경대학교 수산생명의학과) ;
  • 김용석 (부경대학교 수산생명의학과) ;
  • 강주찬 (부경대학교 수산생명의학과)
  • Received : 2011.08.08
  • Accepted : 2012.03.10
  • Published : 2012.04.30
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Abstract

Experiments were carried out to determine the hematological changes in freshwater tilapia Oreochromis niloticus, after water temperature (20, 25 and $30^{\circ}C$) and arsenic concentrations (200, and 400 ppb) exposure 20 days. The RBC count and hematocrit (Ht) were significantly increased in As 400 ppb and water temperature $30^{\circ}C$. Hemoglobin (Hb) was significantly increased under combined As (200 and 400 ppb) and water temperature $30^{\circ}C$. The tilapia exposed to water temperature $30^{\circ}C$ and As concentration 400 ppb increase significantly in calcium concentration. The high concentrations of total protein and glucose concentrations were observed at water temperature $30^{\circ}C$ and As concentration 400 ppb. A significant increment GOT and GPT activities in the tilapia serum noticed at water temperature (25 and $30^{\circ}C$) and As concentration (200, 400 ppb). These results indicate that tilapia can be affected by high water temperature ($30^{\circ}C$) and As concentration (400 ppb) in terms of inorganic matter, organic matter and enzyme activity in serum.

본 연구는 어류의 온도변화 (20, 25 및 $30^{\circ}C$)에 따른 생리적 변동에 미치는 비소 (200 및 400 ppb)의 영향을 파악하기 위하여 틸라피아, Oreochromis niloticus를 대상으로 이들의 혈액학적 변동을 검토하였다. 틸라피아의 적혈구 수는 온도 $30^{\circ}C$의 200 ppb 이상, Ht는 온도 $25^{\circ}C$ 및 $30^{\circ}C$의 400 ppb, Hb 농도는 온도 $30^{\circ}C$의 200 ppb 이상에서 유의한 증를 나타냈다. 혈청 무기성분인 Ca농도는 수온 $30^{\circ}C$의 400 ppb에서 유의하게 증가하였으나, Mg농도는 변동을 나타내지 않았다. 혈청 총 단백질농도는 온도 25 및 $30^{\circ}C$의 비소농도 400 ppb, glucose농도는 온도 $25^{\circ}C$의 200 ppb 비소농도, 온도 25 및 $30^{\circ}C$의 400 ppb 비소농도에서 유의하게 증가하였다. 혈청 GOT활성은 온도 25 및 $30^{\circ}C$의 200 ppb 이상의 비소농도, GPT활성은 온도 20, 25 및 $30^{\circ}C$의 200 ppb 이상의 비소농도에서 유의한 증가를 나타냈다. 이상의 결과 및 논의에서 틸라피아의 혈액성상, 혈청의 무기, 유기농도 및 효소활성은 비소농도 200ppb 이상에서 부분적으로 영향을 받고 있으며, 이 영향은 온도가 높을수록 영향이 클 것으로 생각된다.

Keywords

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