Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Molecular cloning and expression analysis of a C-type lectin in the rock bream, Oplegnathus fasciatus

  • Kwon, Mun-Gyeong (Pathology Division, National Fisheries Research and Development Institute) ;
  • Kim, Ju-Won (Department of Marine Biology & Aquaculture, Institute of Marine Industry, Gyeongsang National University) ;
  • Park, Myoung-Ae (Pathology Division, National Fisheries Research and Development Institute) ;
  • Hwang, Jee-Youn (Pathology Division, National Fisheries Research and Development Institute) ;
  • Park, Hyung-Jun (Department of Marine Biology & Aquaculture, Institute of Marine Industry, Gyeongsang National University) ;
  • Park, Chan-Il (Department of Marine Biology & Aquaculture, Institute of Marine Industry, Gyeongsang National University)
  • Received : 2011.10.17
  • Accepted : 2012.01.05
  • Published : 2012.04.30
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Abstract

C-type lectins are crucial for pathogen recognition, innate immunity, and cell-cell interactions. In this study, a C-type lectin gene was cloned from the rock bream. The full-length RbCTL cDNA was 729 bp with a 429 bp ORF encoding a 164-residue protein. The deduced amino acid sequence of RbCTL had all of the conserved features crucial for its fundamental structure, including the four cysteine residues involved in sulfide bridge formation and potential $Ca^2+$/carbohydrate-binding sites. RbCTL contains a signal peptide one single carbohydrate recognition domain. It showed 29.4% similarity to the C-type lectin of rainbow trout. RbCTL mRNA was predominately expressed in gill and head-kidney tissue and expressed less in peripheral blood leukocytes, trunk-kidney, spleen, liver, intestine and muscle. Expression of RbCTL was differentially upregulated in rock bream stimulated with LPS, Con A/PMA and poly I:C.

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References

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