DOI QR코드

DOI QR Code

Effects of Hyeolbuchukeo-tang on the Estradiol Valerate-induced Polycystic Ovaries and the Conception of Rats

혈부축어탕(血府逐瘀湯)이 Estradiol Valerate로 유발된 흰쥐의 다낭성 난소 및 수태에 미치는 영향

  • Bae, Sang-Jin (Dept. of Oriental Gynecology, College of Oriental Medicine, Se-Myung University) ;
  • Kim, Hyung-Jun (Dept. of Oriental Gynecology, College of Oriental Medicine, Se-Myung University) ;
  • Lee, Dong-Nyung (Dept. of Oriental Gynecology, College of Oriental Medicine, Se-Myung University)
  • 배상진 (세명대학교 한의과대학 부인과학교실) ;
  • 김형준 (세명대학교 한의과대학 부인과학교실) ;
  • 이동녕 (세명대학교 한의과대학 부인과학교실)
  • Received : 2012.04.27
  • Accepted : 2012.05.15
  • Published : 2012.05.25

Abstract

Objectives: This study was designed to investigate the effects of Hyeolbuchukeotang( HCT) on the Estradiol Valerate(EV) induced Polycystic Ovaries and the Implantation of rats. Methods: PCO was induced by single intramuscular(IM) injection with EV in female rats. Normal group(n=8) were injected with sesame oil and orally administrated distilled water for 60 days. Control group(n=8) were injected with EV and orally administrated distilled water for 60 days. HCT treated group(n=8) were injected with EV and orally administrated HCT for 60 days. At the end day of experiment, we measured weights of body, ovaries, adrenal glands, uterus and contents of serum LH, FSH, ADD. The histomorphometrical changes of ovaries were also evaluated. And we observed the NGF and CRF expression by immunohistochemistry. Also we observed the mating and count the number of implantation. Results: - The weights(mg) of ovaries in HCT treated group($58.9{\pm}6.5$) were significantly increased (p<0.01) compared with control group ($42.3{\pm}8.5$). - The number of mature follicles in HCT treated group($9.8{\pm}2.6$) was significantly increased (p<0.01) compared with control group($6.1{\pm}2.1$). - The number of cystic follicles in HCT treated group($1.6{\pm}1.2$) was significantly decreased (p<0.01) compared with control group($3.8{\pm}1.5$). - The value of serum FSH(mIU/ml) in HCT treated group($3.870{\pm}2.151$) was significantly decreased(p<0.05) compared with control group($10.476{\pm}6.294$). - The expression of NGF-immunoreactive cells in the ovarian cells in HCT treated group was lesser observed than control group. - The number of implantation in HCT treated group($7.4{\pm}1.8$) was significantly increased (p<0.05) compared with control group($3.1{\pm}4.4$). Conclusions: Hyeolbuchukeo-tang(HCT) is effect on polycystic ovaries and the implantation of rats by EV-induced.

Keywords

References

  1. 대한산부인과학회 교과서편찬위원회. 부인과학. 서울:고려의학. 2007:63, 362.
  2. Nestler JE. Polycystic ovary syndrome: a disorder for the generalist. Fertil Steri 1998;70:811-2. https://doi.org/10.1016/S0015-0282(98)00318-5
  3. Stein IF, Levental ML. Amenorrhea associated with bilateral polycystic ovaries. Am J Obstet Gynecol. 1935; 29:181-91. https://doi.org/10.1016/S0002-9378(15)30642-6
  4. Lara HE, et al. An increased intraovarian synthesis of nerve growth factor and its low affinity receptor is a principal component of steroid-induced polycystic ovary in the rat. Endocrinology. 2000; 141:1059-72. https://doi.org/10.1210/endo.141.3.7395
  5. S Hisano, et al. Reciprocal synaptic relations between CRF-immunoreactive and TRH-immunoreactive neurons in the paraventricular nucleus of the rat hypothalamus. Brain Res. 1993;620 :343-6. https://doi.org/10.1016/0006-8993(93)90178-P
  6. Krysiak R, et al. Update on the management of PCOS. Pharmacological Reports. 2006;58(5):614-25.
  7. 김동일, 윤종원, 이태균. 다낭성 난소증후군에 관한 문헌적 고찰. 대한한방부인과학회지. 1997;10(1):73-91.
  8. 吳桂芳, 李祥云. 中醫學治療多囊卵巢 綜合症硏究槪況. 河南中醫. 1994;14(2):123-5.
  9. 王淸任. 醫林改錯. 北京:中國中醫藥出版社. 1995:24-8.
  10. J R Brawer, M Munoz, R Farookhi. Development of the PCO in the estradiol valerate- treated rat. Biology of Reproduction. 1986;35:647-55. https://doi.org/10.1095/biolreprod35.3.647
  11. Elisabet Stener-Victorin, et al. Rats with steroid-induced PCO develop HTN and increased sympathetic nervous system activity. Reproductive Biology and Endocrinology. 2005;3:44. https://doi.org/10.1186/1477-7827-3-44
  12. Adam H. et al. Ultrasound assessment of the polycystic ovary: international consensus definitions. Human Reproduction Update. 2003;9:505-14. https://doi.org/10.1093/humupd/dmg044
  13. 김 탁. 다낭성 난소 증후군의 최신 지견. 대한산부인과학회 연수강좌. 2002;31:38-44.
  14. 김정훈. 다낭성난소증후군 치료의 최신지견. Korean Journal of Obstetrics and Gynecology. 2005;48(8):1852.
  15. Baillargeon JP. Use of insulin sensitizers in polycystic ovarian syndrome. Current Opinion in Investigational Drugs. 2005;6:1012-22.
  16. Gerard S. Conway, et al. Effects of Luteinizing Hormone, Insulin, Insulin-like Growth Factor-I and Insulin-like Growth Factor Small Binding Protein 1 in the Polycystic Ovary Syndrome. Clinical Endocrinology. 1990;33:596-603.
  17. Zawadzki JK, Dunaif A. Diagnostic criteria for polycystic ovary syndrome: towards a rational approach. In: Dunaif A, Givens JR, Haseltine FP, Merriam GR, editors. Polycystic Ovary Syndrome. 3rd ed. Boston: Blackwell Scientific Publications. 1992;377-84.
  18. The Rotterdam ESHRE/ASRM Sponsored PCOS Consensus Workshop Group. Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome. Fertil Steril. 2004;81:19-25.
  19. S. Hahn, et al. Clinical and biochemical characterization of women with PCOS in North Rhine-Westphalia. Hormone and Metabolic Research. 2005;37:438-44. https://doi.org/10.1055/s-2005-870236
  20. Richard S. Legro, et al. Prevalence and predictors of risk for type 2 diabetes mellitus and impaired glucose tolerance in PCOS: a prospective, controlled study in 254 affected women. Journal of Clinical Endocrinology & Metabolism. 1999;84:165-9.
  21. Ann E. Taylor, et al. Determinants of abnormal gonadotropin secretion in clinical defined women woth PCOS. Journal of Clinical Endocrinology & Metabolism. 1997;82:2248-56.
  22. Gharani N, et al. Association of the steroid synthesis gene $CYP11{\alpha}$ with polycystic ovary syndrome and hyperandrogenism. Hum Mol Genet. 1997;6:397-402. https://doi.org/10.1093/hmg/6.3.397
  23. Waterworth DM, et al. Linkage and association of insulin gene VNTR regulatory polymorphism with polycystic ovary syndrome. Lancet 1997;349 :986-90. https://doi.org/10.1016/S0140-6736(96)08368-7
  24. 이지영, 등. 한국인 여성에서 다낭성난소증후군의 발생 위험도와 CYP17유전자 다형성과의 관련성에 관한 연구. Korean Journal of Obstetrics and Gynecology. 2005;48(5):1306.
  25. 정철회, 등. Dehydroepiandrosterone로 유도된 흰쥐의 다낭성난소에서 cDNA array를 이용한 유전자발현분석. 대한산부인과학회지. 2004;47(10):1931.
  26. 강길전. 여성생식의학. 서울:대관출판사. 1998:219-30.
  27. 윤소영, 강명자. 다낭성 난소증후군 환자의 한방 치료에 대한 임상적 고찰. 대한한방부인과학회지. 2000;13(2):437-51.
  28. Elisabet Stener-Victorin, et al. Effects of eletro-acupuncture on nerve growth factor and ovarian morphology in rats with experimentally induced PCO. Biology of Reproduction. 2000;63(5) :1497-503. https://doi.org/10.1095/biolreprod63.5.1497
  29. E. Stener-Victorin, et al. Effects of electro-acupuncture on corticotropinreleasing factor in rats with experimentally induced polycystic ovaries. Neuropeptides. 2001;35(5&6) :227-31. https://doi.org/10.1054/npep.2002.0878
  30. Elisabet Stener-Victorin. Acupuncture in reproductive medicine: Overview and summary of recent studies. International Congress Series. 2002; 1238:149-56. https://doi.org/10.1016/S0531-5131(02)00420-X
  31. 권세라, 등. 삼음교 자궁혈 전침 자극이 백서의 다낭성 난소에 미치는 영향. 대한한방부인과학회지. 2003;16(2):76-86.
  32. L. Manni, S. et al. Effects of exrecise on ovarian morphology and expression of nerve growth factor and ${\alpha}1-and$ $\beta$ 2-adrenergic receptors in rats with steroid-induced polycystic ovaries. Journal of Neuroendocrinology. 2005; 17:846-58.
  33. 양동선, 등. 향부자가 estradiol valerate로 유발된 백서의 다낭성 난소에 미치는 영향. 대한한방부인과학회지. 2010;23(4):35-46.
  34. 구희준, 조성희. 皂角刺가 estradiol valerate로 유발된 白鼠의 다낭성 난소에 미치는 영향. 대한한방부인과학회지. 2010;23(2):71-84.
  35. Sok Cheon Pak, et al. Effect of Korean red ginseng extract in a steroidinduced polycystic ovary murine model. Arch Pharm Res. 2009;32(3):347-52. https://doi.org/10.1007/s12272-009-1306-y
  36. 양승정, 등. 蒼附導痰湯이 estradiol valerate로 유발된 백서의 다낭성 난소에 미치는 影響. 대한한방부인과학회지. 2002;15(2):1-11.
  37. 유영기, 등. 腎氣丸合蒼附導痰湯이 estradiol valerate로 유발된 흰쥐의 다낭성 난소에 미치는 영향. 대한한방부인과학회지. 2007;20(1):84-98.
  38. 남은정, 등. 定經湯이 estradiol valerate로 유발된 흰쥐의 다낭성 난소에 미치는 영향. 대한한방부인과학회지. 2007;20(1):99-113.
  39. 김희주, 김윤상, 임은미. 蒼附六君湯이 estradiol valerate로 유발된 흰쥐의 다낭성 난소에 미치는 영향. 대한한방부인과학회지. 2008;21(2):1-16.
  40. 이연경, 등. 歸脾湯이 estradiol valerate로 유발된 흰쥐의 다낭성 난소에 미치는 영향. 대한한방부인과학회지. 2008;21(3):60-74.
  41. 윤문희, 등. 開鬱二陳湯이 Estradiol Valerate로 유도된 흰쥐의 다낭성 난소 발달에 미치는 영향. 대한한방부인과학회지. 2010;23(2):1-19.
  42. 김종대, 등. 五積散이 Estradiol Valerate로 유발된 다낭성 난소 모델에서 난포의 성숙 및 NGF발현에 미치는 영향. 대한한방부인과학회지. 2011;24(1):27-41.
  43. 전국한의과대학 병리학 교실 編. 동의병리학 서울:一中社. 1999:152-65, 272-4, 424-6.
  44. 宋炳基. 韓方婦人科學. 서울:행림출판사. 1995:17-9.
  45. 한의부인과학교재편찬위원회. 韓醫婦人科學(上). 서울:도서출판 정담. 2002:61, 101, 190-1.
  46. Gary E. Ackerman, et al. Aromatization of Androstenedione by Human Adipose Tissue Stromal Cells in Monolayer Culture. The Journal of Clinical Endocrinology & Metabolism. 1981; 53(2):412. https://doi.org/10.1210/jcem-53-2-412
  47. L.W. Swanson, et al. Organization of Ovine Corticotropin-Releasing Factor Immuno -reactive Cells and Fibers in the Rat Brain: An Immunohistochemical Study. Neuro Endocrinology. 1983;36(3):165-86.
  48. 한혜숙, 등. 血府逐瘀湯加味方의 炎症病理因子와 酸化力損傷에 미치는 영향. 대한한방부인과학회지. 2008;21(1):99-116.
  49. 임현정, 등. 血府逐瘀湯加味方의 抗血栓作用에 대한 實驗的硏究. 대한 한방부인과학회지. 2008;21(1):117-33.
  50. 문나영, 김동철, 백승희. 혈부축어탕이 자궁근종세포의 증식억제와 Apoptosis 관련 유전자 발현에 미치는 영향. 대한한방부인과학회지. 2006;19(2):186-98.
  51. 洪喜鐸, 金浡烈, 李泰均. 血府逐瘀湯이 免疫機能에 미치는 영향. 대한한방부인과학회지. 1996;9(1):177-89.
  52. 박종형, 등. 血府逐瘀湯이 血栓生成關與因子에 미치는 영향. 대한한방내과학회지. 2000;21(5):819-27.
  53. 최유경, 등. 血府逐瘀湯이 스트레스에 미치는 영향. 대한한방내과학회지. 2004;26(3):162-75.
  54. 崔順子. 血府逐瘀湯이 STZ 糖尿病Rat의 高脂血證에 미치는 영향. 경원대학교 대학원. 2004.
  55. 梁秀光. 血府逐瘀湯이 STZ 유도 糖尿病상태의 肝機能에 미치는 영향. 경원대학교 대학원. 2004.
  56. 李眞華, 등. 血府逐瘀湯이 癌轉移抑制에 미치는 영향. 대한한방종양의학회지. 1999;5(1):61-75.
  57. 馬敏珠. 血府逐瘀湯治療子宮內膜異位症. 上海中醫藥雜誌. 1993;2:16-7.
  58. 陳子富. 宋向元治瘀經驗發揮. 北京中醫學院學報. 1986;3:149-17.
  59. Anderson E, Lee GY, O'Brien K. PCO condition in dehydroepiandrosterone -treated rat model: Hyperandrogenism and the resumption of meiosis are major initial events associated with cystogenesis of antral follicles. Reproductive Biology. 1998;249(1) :44-53.
  60. Louise Mannerås, et al. A new rat model exhibiting both ovarian and metabolic characteristics of polycystic ovary syndrome. Endocrinology. 2007; 148(8):3781-91. https://doi.org/10.1210/en.2007-0168
  61. Farookhi R, Hemmings R, Brawer JR. Unilateral ovariectomy restores ovulatory cyclicity in rats with a polycystic ovarian condition. Biol Reprod. 1985; 32(3):530-40. https://doi.org/10.1095/biolreprod32.3.530
  62. 김정훈. PCOS 치료의 최신지견. Korean Journal of Ob & Gy. 2005;48(8):1853.
  63. 구병삼. 임상 부인과 내분비학 제2판. 서울:고려의학. 2001:87
  64. Levi MR. The nerve growth factor 35 years later. Science. 1987;237 :1154-62. https://doi.org/10.1126/science.3306916
  65. G.A. Dissen, et al. Intraovarian excess of nerve growth factor increases androgen secretion and disrupts estrous cyclicity in the rat. Endocrinology. 2000;141(3):1073-81. https://doi.org/10.1210/endo.141.3.7396
  66. H.E. Lara, et al. An increased intraovarian synthesis of nerve growth factor and its low affinity receptor is a principal component of steroid-induced polycystic ovary in the rat. Endocrinology. 2000; 141:1059-72. https://doi.org/10.1210/endo.141.3.7395