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Investigation of the Performance of Spectral Domain Optical Doppler Tomography with High-speed Line Scanning CMOS Camera and Its Application to the Blood Flow Measurement in a Micro-tube

  • Park, Cheol Woo (School of Mechanical Engineering, Kyungpook National University) ;
  • Lee, Changho (School of Electrical Engineering and Computer Science, Kyungpook National University) ;
  • Lim, SooHee (School of Mechanical Engineering, Kyungpook National University) ;
  • Ni, Aleksey (School of Mechanical Engineering, Kyungpook National University) ;
  • An, Jin Hyo (School of Mechanical Engineering, Kyungpook National University) ;
  • Lee, Ho (School of Mechanical Engineering, Kyungpook National University) ;
  • Bae, Jae Sung (Department of Physiology, School of Medicine, Kyungpook National University) ;
  • Kim, Jeehyun (School of Electrical Engineering and Computer Science, Kyungpook National University)
  • Received : 2011.10.07
  • Accepted : 2012.03.20
  • Published : 2012.06.25

Abstract

In this study, the feasibility of spectral domain optical Doppler tomography for measuring blood flow characteristics in a micro-tube was demonstrated through several experiments. The use of an SD-ODT system in blood flow measurement can provide high resolution images (5 microns resolution). We prepared three capillary tubes to reveal the effect of different concentrations of hematocrit ratio (HR). One tube serves as the control. The two other tubes contained different concentrations of HR (5%, 25%). Three different capillary tube inlet flow velocities were tested in the present study. The Reynolds number (Re) which is based on the capillary tube inner diameter ranges from Re=6 to 48. We calculated a Doppler shift of the power spectrum of the temporal interference fringes with Kasai autocorrelation function to achieve the velocity profile of the flow. As a result, SD-ODT systems could not detect the cell depletion layer in the present study due to the limitation of spatial resolution. Nevertheless, these systems were proven to be capable of observing the RBCs of blood.

Keywords

References

  1. R. J. Adrian, "Particle-imaging techniques for experimental fluid mechanics," Annu. Rev. Fluid Mech. 23, 261-304 (1991). https://doi.org/10.1146/annurev.fl.23.010191.001401
  2. N. Matsunaga, Y. Sugihara, T. Komatsu, and A. Masuda, "Quantitative properties of oscillating-grid turbulence in a homogeneous fluid," Fluid Dynamics Research. 25, 147-165 (1999). https://doi.org/10.1016/S0169-5983(98)00034-3
  3. A. F. Fercher, W. Drexler, C. K. Hitzenberger, and T. Lasser, "Optical coherence tomography-particles and application," Rep. Prog. Phys. 66, 239-303 (2003). https://doi.org/10.1088/0034-4885/66/2/204
  4. D. Huang, E. A. Swanson, C. P. Lin, J. S. Shuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Pulialito, and J. G. Fujimoto, "Optical coherence tomography," Science 254, 1178-1181 (1991). https://doi.org/10.1126/science.1957169
  5. N. H. Cho, U. Jung, H. I. Kwon, H. Jeong, and J. Kim, "Development of SD-OCT for imaging the in vivo human tympanic membrane," J. Opt. Soc. Korea 15, 74-77 (2011). https://doi.org/10.3807/JOSK.2011.15.1.074
  6. U. Jung, N. H. Cho, S. H. Kim, H. Jeong, J. Kim, and Y. C. Ahn, "Simple spectral calibration method and its application using an index array for swept source optical coherence tomography," J. Opt. Soc. Korea 15, 386-393 (2011). https://doi.org/10.3807/JOSK.2011.15.4.386
  7. K. V. Larin, M. Motamedi, M. S. Eledrisi, and R. O. Esenaliev, "Noninvasive blood glucose monitoring with optical coherence tomography," Diabetes Care. 25, 2263-2267 (2002). https://doi.org/10.2337/diacare.25.12.2263
  8. Z. Chen, T. E. Milner, D. Dave, and J. S. Nelson, "Optical Doppler tomographic imaging of fluid flow velocity in highly scattering media," Opt. Lett. 22, 64-66 (1997). https://doi.org/10.1364/OL.22.000064
  9. J. A. Izatt, M. D. Kulkarni, S. Yazdanfar, J. K. Barton, and A. J. Welch, "In vivo bidirectional color Doppler flow imaging of picoliter blood volumes using optical coherence tomography," Opt. Lett. 22, 1439-1441 (1997). https://doi.org/10.1364/OL.22.001439
  10. X. Xu, Y. C. Ahn, and Z. Chen, "Feasibility of Doppler variance imaging for red blood cell aggregation characterization," J. Biomed. Opt. 14, 060507 (2009). https://doi.org/10.1117/1.3275464
  11. X. Xu, L. Yu, and Z. Chen, "Velocity variation assessment of red blood cell aggregation with spectral domain Doppler optical coherence tomography," Ann. Biomed. Eng. 38, 3210-3217 (2010). https://doi.org/10.1007/s10439-010-0066-7
  12. Z. Chen, T. E. Milner, S. Srinivas, X. Wang, A. Malekafzali, M. J. C. Gemert, and J. S. Nelson, "Noninvasive imaging of in vivo blood flow velocity using optical Doppler tomography," Opt. Lett. 22, 1119-1121 (1997). https://doi.org/10.1364/OL.22.001119
  13. X. J. Wang, T. E. Milner, and J. S. Nelson, "Characterization of fluid flow velocity by optical Doppler tomography," Opt. Lett. 20, 1337-1339 (1995). https://doi.org/10.1364/OL.20.001337
  14. V. Westphal, S. Yazdanfar, A. M. Rollins, and J. A. Izatt, "Real-time, high velocity-resolution color Doppler optical coherence tomography," Opt. Lett. 27, 34-36 (2002). https://doi.org/10.1364/OL.27.000034
  15. C. Kasai, K. Namekawa, A. Koyano, and R. Omoto, "Realtime two-dimensional blood flow imaging using an autocorrelation technique," IEEE Trans. Sonics. Ultrason. 32, 458-464 (1985). https://doi.org/10.1109/T-SU.1985.31615
  16. A. Mariampillai, B. A. Standish, N. R. Munce, C. Randall, G. Liu, J. Y. Jiang, A. E. Cable, I. A. Vitkin, and V. X. D. Yang, "Doppler optical cardiogram gated 2D color flow imaging at 1000 fps and 4D in vivo visualization of embryonic heart at 45 fps on a swept source OCT system," Opt. Express 2, 1627-1638 (2007).
  17. F. M. White, Fluid Mechanics (McGraw-Hill Press, USA, 1986).
  18. C. W. Park, S. H. Shin, G. M. Kim, J. H. Jang, and Y. H. Gu, "A hemodynamic study on a marginal cell depletion layer of blood flow inside a microchannel," Key Eng. Materials 326, 863-866 (2006). https://doi.org/10.4028/www.scientific.net/KEM.326-328.863
  19. R. N. Maccallum, W. O'Bannon, J. D. Hellums, C. P. Alfrey, and E. C. Lynch, Rheology of Biological Systems (C. C. Thomas, Springfield, USA, 1973).