Journal of Trauma and Injury

The Korean Society of Traumatology (대한외상학회)
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Effect of Therapeutic Hypercapnia on Systemic Inflammatory Responses in Hemorrhagic Shock in Rats

랫드의 출혈성 쇼크 모델에서 치료적 고탄산혈증이 전신적 염증 반응에 미치는 영향

  • Kang, Kyeong Won (Department of Emergency Medicine, Seoul National University Bundang Hospital) ;
  • Jo, You Hwan (Department of Emergency Medicine, Seoul National University Bundang Hospital) ;
  • Kim, Kyuseok (Department of Emergency Medicine, Seoul National University Bundang Hospital) ;
  • Lee, Jae Hyuk (Department of Emergency Medicine, Seoul National University Bundang Hospital) ;
  • Rhee, Joong Eui (Department of Emergency Medicine, Seoul National University Bundang Hospital)
  • 강경원 (분당서울대학교병원 응급의학과) ;
  • 조유환 (분당서울대학교병원 응급의학과) ;
  • 김규석 (분당서울대학교병원 응급의학과) ;
  • 이재혁 (분당서울대학교병원 응급의학과) ;
  • 이중의 (분당서울대학교병원 응급의학과)
  • Received : 2012.02.15
  • Accepted : 2012.03.06
  • Published : 2012.06.30
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Abstract

Purpose: This study was performed to investigate whether therapeutic hypercapnia could attenuate systemic inflammatory responses in hemorrhagic shock in rats. Methods: Male Sprague-Dawley rats were mechanically ventilated and underwent pressure-controlled (mean arterial pressure: $38{\pm}1$ mmHg) hemorrhagic shock. At 10 minutes after the induction of hemorrhagic shock, the rats were divided into the normocapnia ($PaCO_2$=35-45 mmHg, n=10) and the hypercapnia ($PaCO_2$=60-70 mmHg) groups. The $PaCO_2$ concentration was adjusted by using the concentration of inhaled $CO_2$ gas. After 90 minutes of hemorrhagic shock, rats were resuscitated with shed blood for 10 minutes and were observed for 2 hours. The mean arterial pressure (MAP) and the heart rate were monitored continuously, and the results of arterial blood gas analyses, as well as the plasma concentrations of interleukin (IL)-6, IL-10, and nitrite/nitrate were compared between the normocapnia and the hypercapnia groups. Results: The MAP and the heart rate were not different between the two groups. The plasma concentration of IL-6 was significantly lower in the hypercapnia group than in the normocapnia group (p<0.05). The IL-10 concentration was not different and the IL-6 to IL-10 ratio was significantly lower in the hypercapnia group compared to the normocapnia group. The plasma nitrite/nitrate concentration of the hypercapnia group was lower than that of the normocapnia group. Conclusion: Therapeutic hypercapnia attenuates systemic inflammatory responses in hemorrhagic shock.

Keywords

Acknowledgement

Supported by : 분당서울대학교병원

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