Biomaterials Research (생체재료학회지)

Korean Society for Biomaterials (한국생체재료학회)
권호 표지

Migration of Human Dermal Fibroblast is Affected by the Diameter of the Electrospun PLGA Fiber

  • Kim, Min Sung (Cellbiocontrol Laboratory, Department of Medical Engineering) ;
  • Kim, Dohyun (Cellbiocontrol Laboratory, Department of Medical Engineering) ;
  • Kang, Jae Kyeong (Cellbiocontrol Laboratory, Department of Medical Engineering) ;
  • Lee, Jeong-Hyun (Cellbiocontrol Laboratory, Department of Medical Engineering) ;
  • Kim, Hye Lee (Cellbiocontrol Laboratory, Department of Medical Engineering) ;
  • Koo, Min-Ah (Cellbiocontrol Laboratory, Department of Medical Engineering) ;
  • Lee, Mi Hee (Cellbiocontrol Laboratory, Department of Medical Engineering) ;
  • Park, Jong-Chul (Cellbiocontrol Laboratory, Department of Medical Engineering)
  • Published : 2012.12.01
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Abstract

Cell migration is an essential activity of the cells in various biological phenomena such as embryonic development, wound healing of damaged tissue, capillary vascularization in angiogenesis and migration of leukocytes to kill the bacteria around the wound site. The properties of nanofibrous surface enhancing cell adhesion, proliferation, migration and differentiation are necessary for application in tissue engineering. Recently, fabricated scaffolds at the nanometer scale are very similar to the architecture of natural human tissue, because of the development of nanofibers. In this study, we observed different cell migration behaviors on PLGA nanofibers with different diameters. 0.4 ${\mu}m$ and 1.4 ${\mu}m$ PLGA fibers were fabricated by electrospinning. Adhesion of neonatal human dermal fibroblasts (nHDFs) on the PLGA scaffolds was quantified by MTT assay. Real time observation system was used to analyze the migration of nHDF on the 0.4 ${\mu}m$ and 1.4 ${\mu}m$ PLGA scaffolds. There are no significant differences in cell attachment between 0.4 ${\mu}m$ and 1.4 ${\mu}m$ PLGA nanofibers. However, the migration was affected by the thickness of the PLGA fiber. The cells were migrated along with the 0.4 ${\mu}m$ PLGA fiber but did not cross 1.4 ${\mu}m$ PLGA fiber. In this research, it would be evaluated that different diameter of electrospun PLGA fiber effect on the cell migration and proliferation, and it could be applied for the development of the fibrous scaffold in tissue engineering.

Keywords

References

  1. S. Li, J. L. Guan, S. Chien, "Biochemistry and biomechanics of cell mortility," Annu. Rev. Biomed. Eng., 7, 105-150 (2005). https://doi.org/10.1146/annurev.bioeng.7.060804.100340
  2. D. A. Lauffenburger and A. F. Horwitz, "Cell migration:a physically integrated molecular process," Cell, 84, 359-369 (1996). https://doi.org/10.1016/S0092-8674(00)81280-5
  3. R. Vasita and D. S. Katti, "Nanofibers and their applications in tissue engineering," Int. J. Nanomedicine, 1, 15-30 (2006). https://doi.org/10.2147/nano.2006.1.1.15
  4. J. R. Venugopal, Y. Zhang, and S. Ramakrishna, "In vitro culture of human dermal fibroblastson electrospun polycaprolactone collagen nanofibrous membrane," Artif. Organs., 6, 440-446 (2006).
  5. E. K. Yim and K. W. Leong, "Significance of synthetic nanostructures in dictating cellular response," Nanomedicine, 1, 10-21 (2005). https://doi.org/10.1016/j.nano.2004.11.008
  6. N. T. Hiep and B.T. Lee, "Electro-spinning of PLGA/PCL blends for tissue engineering and their biocompatibility," J. Mater. Sci. Mater. Med., 21, 1969-1978 (2010). https://doi.org/10.1007/s10856-010-4048-y
  7. J. D. and D. H. Reneker, "Electrospinning process and applications of electrospun fibers," J. Electrostat., 35, 151-160 (1995). https://doi.org/10.1016/0304-3886(95)00041-8
  8. B. Duan, X. Yuan, Y. Zhu, Y. Zhang, X. Li and Y. Zhang, "A nanofibrous composite membrane of PLGA-chitosan/PVA prepared by electrospinning," J. Eur. Polym., 42, 2013-2022 (2006). https://doi.org/10.1016/j.eurpolymj.2006.04.021
  9. C. M. Vaz, S. V. Tuijil, C. V. C. Bouten and F. P. T. Baaijens, "Design of scaffolds for blood vessel tissue engineering using a multi-layering electrospinning technique," Acta. Biomater., 1, 575-582 (2005). https://doi.org/10.1016/j.actbio.2005.06.006
  10. Y. Zhang, J. R. Venugopal, A. El-Turki, S. Ramakrishna, B. Su and C. Lim, "Electrospun biomimetic nanocomposite nanofibers of hydroxyapatite/chitosan for bone tissue engineering," Biomaterials, 29, 4314-4322 (2008). https://doi.org/10.1016/j.biomaterials.2008.07.038
  11. C. A. Bashur, L. A. Dahlgren and A. S. Goldstein, "Effect of fiber diameter and orientation on fibroblast morphology and proliferation on electrospun poly(D,L-lactic-co-glycolic acid) meshes," Biomaterials, 27, 5681-5688 (2006). https://doi.org/10.1016/j.biomaterials.2006.07.005
  12. H. Fong, I. Chun and D. H. Reneker, "Beaded nanofibers formed during electrospinning," Polymer, 40, 4585-4592 (1999). https://doi.org/10.1016/S0032-3861(99)00068-3
  13. P. Gupta, C. Elkins, T. E. Long and G. L. Wilkes, "Electrospinning of linear homopolymers of poly(methyl methacrylate): exploring relationships between fiber formation, viscosity, molecular weight and concentration in a good solvent," Polymer, 46, 4799-4810 (2005). https://doi.org/10.1016/j.polymer.2005.04.021
  14. L. S. Nair, S. Bhattacharyya, J. D. Bender, Y. E. Greish, P. W. Brown and H. R. Allcock, "Fabrication and optimization of methylphenoxy substituted polyphosphazene nanofibers for biomedical applications," Biomacromolecules, 5, 2212-2220 (2004). https://doi.org/10.1021/bm049759j
  15. H. Fong, I. Chun and D.H. Reneker, "Beaded nanofibers formed during electrospinning," Polymer, 40, 4585-4592(1999). https://doi.org/10.1016/S0032-3861(99)00068-3
  16. V. M. Entov and L. E. Shmaryan, "Numerical modeling of the capillary breakup of jets of polymer liquids," Fluid Dynamics, 32, 696-703 (1997).
  17. R. Jaeger, H. Schonherr and J. Vancso, "Chain packing in electrospun poly-(ethylene oxide) visualized by atomic force microscopy," Macromolecules, 29, 7634-7636 (1996). https://doi.org/10.1021/ma9610673
  18. R. Jaeger, M. Bergshoef, C. Batlle, H. Schonherr and J. Vancso, "Electrospinning of ultra-thin polymer fibers," Macromol. Symp., 127, 141-150 (1998).
  19. J. Y. Kim and D. W. Cho, "Blended PCL/PLGA scaffold fabrication using multi-head deposition system," Microelectron. Eng., 86, 1447-1450 (2009). https://doi.org/10.1016/j.mee.2008.11.026
  20. M. Mastrogiacomo, A. Muraglia, V. Komlev, F. Peyrin, F. Rustichelli, A. Crovace and R. Cancedda, "Tissue engineering of bone: search for a better scaffold," Orthod. Craniofac, Res., 8, 277-84 (2005). https://doi.org/10.1111/j.1601-6343.2005.00350.x
  21. D. Falconneta, G. Csucsb, H. M. Grandina and M. Textor, "Surface engineering approaches to micropattern surfaces for cellbased assays," Biomaterials, 27, 3044-3063(2006). https://doi.org/10.1016/j.biomaterials.2005.12.024
  22. H. RO, "Integrins: bidirectional, allosteric signaling machines," Cell, 110, 673-87 (2002). https://doi.org/10.1016/S0092-8674(02)00971-6
  23. G. Kumar, C. C. Ho and C. C. Co, "Guiding cell migration using one-way micropattern arrays," Adv. Mater., 19, 1084-1090 (2007). https://doi.org/10.1002/adma.200601629