생체재료학회지 (Biomaterials Research)

  • 제16권2호
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  • Pages.86-95
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  • 2012
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  • 1226-4601(pISSN)
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  • 2055-7124(eISSN)
한국생체재료학회 (Korean Society for Biomaterials)
권호 표지

연골재생 및 연골세포에 대한 혈소판풍부혈장의 기능 연구

Effects of Platelet-rich Plasma on Chondrocyte and Cartilage Regeneration

  • 이혜림 (건국대학교 수의학대학 임상병리학) ;
  • 김석영 (영남대학교 공과대학 신소재공학부) ;
  • 안면환 (영남대학교 의과대학 정형외과학) ;
  • 도선희 (건국대학교 수의학대학 임상병리학)
  • Lee, Hye-Rim (Department of Clinical Pathology, College of Veterinary Medicine, Konkuk University) ;
  • Kim, Sukyoung (School of Materials Science and Engineering, Yeungnam University) ;
  • Ahn, Myun-Whan (Department of Orthopedic surgery, College of Medicine, Yeungnam University) ;
  • Do, Sun Hee (Department of Clinical Pathology, College of Veterinary Medicine, Konkuk University)
  • 발행 : 2012.06.01
⟨ 이전 논문 다음 논문 ⟩

초록

Cartilage is a tissue with unique functional properties that are very dependent on its extracellular matrix (ECM), with collagens and proteoglycans as its main constituents. Cartilage injuries are common, frequently lead to disability, and have a low intrinsic regenerative ability that may result from the avascular nature of cartilage, which prevents access to wound healing process. The clinical effectiveness of therapeutic application has been commonly tested in articular osteochondral defects, and a variety of reparative techniques have been used in an attempt to improve the self-regenerative capabilities of cartilage. Three-dimensional (3D) scaffolds like hydrogel have become a major research focus for cell and drug delivery in tissue engineering. Presently, we investigated the regenerative potency of platelet-rich plasma (PRP) combined with a chondrocyte/hydrogel composite scaffold in the repair of cartilage defects using an animal model. The efficacy of 3D culture on the proliferation and maturation of chondrocytes was assessed. The results provide valuable reference for the clinical application of a hydrogel scaffold for cartilage regeneration, as well as the use of autologous PRP to improve cellular proliferation and maturation of hyaline cartilage repair. However, the meniscal chondrocytes cultured with 10% PRP revealed down regulation of chondrogenic molecules indicating an increase of type I collagen and decrease of type II collagen relation with up-regulation of matrix metalloproteinases 1 and 3 as compared to the normal condition. Conclusively, these results suggested PRP could be diverse roles in regenerative medicine and cellular homeostatsis depending to characteristics of cells and tissues.

키워드

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