한국지반환경공학회 논문집 (Journal of the Korean GEO-environmental Society)

  • 제13권2호
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  • Pages.41-47
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  • 2012
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  • 1598-0820(pISSN)
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  • 2714-1233(eISSN)
한국지반환경공학회 (Korean Geo-Environmental Society)
권호 표지

적색 발광다이오드(Light Emitting Diode)를 이용한 클로렐라 배양에 미치는 영향인자 분석: 빛세기, 공기 및 이산화탄소 주입

Effects of Limiting Factors on Cultivation of Chlorella Sp. under Red Light Emitting Diode: Light Intensity, Blowing of Air or Carbon Dioxide

  • 최보람 (부경대학교 환경해양대학 환경공학과) ;
  • 이태윤 (부경대학교 환경해양대학 환경공학과)
  • 발행 : 2012.02.01
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구는 담수미세조류의 일종인 클로렐라를 적색 발광다이오드를 이용하여 효율적으로 배양하기 위한 조건을 찾기 위해 수행되었다. 클로렐라 배양에 최적인 광량을 찾기 위해 광량을 조절하여 클로렐라가 포함된 반응기에 각각 조사하여 성장속도 및 셀농도를 측정하였다. 적색파장 사용 시, 광량이 증가할수록 성장속도는 광량에 비례하여 증가하였으나 셀농도는 지속적으로 감소하였다. 공기 공급에 따른 성장특성을 살펴보기 위해 반응기에 미세공기를 연속적으로 주입하였는데, 반응속도 및 셀농도는 공기를 주입하지 않은 경우에 비해 2배와 10배 증가함을 알 수 있었다. 고농도의 이산화탄소 주입의 경우에는 배지의 pH가 3이하로 저하되어 클로렐라의 성장이 감소하는 것을 알 수 있었다. 본 연구를 통해 클로렐라를 효과적으로 배양하기 위해서는 적색파장의 발광다이오드를 광원으로 사용하고 반응기에 공기를 연속적으로 주입하는 것이 효과적임을 알 수 있었다.

The purpose of this study was to determine optimum condition for the cultivation of Chlorella sp. FC-21 using red light emitting diodes (LED). Specific growth rate and cell concentration were measured for the reactors at the illuminations of different light intensity of red LED. Under the illumination of red LED, specific growth rate increased as light intensity increased but cell concentrations decreased. To determine beneficial effect of aeration to cell cultivation, micro-air bubbles were aerated at 0.7 vvm in the reactor at the illumination of red LED. Two and ten times greater specific growth rate and cell concentration were obtained when aeration was applied. In case of blowing of carbon dioxide, pH of culture medium decreased below to pH 3, which resulted in decreases of cell concentration. From this study, we found that red LED with aeration were the most appropriate light source for the cultivation of Chlorella sp. FC-21.

키워드

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