Journal of the Korea Organic Resources Recycling Association (유기물자원화)

Korea Organic Resources Recycling Association (유기성자원학회)
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Biohydrogen Production from Sugar Manufacturing Wastewater and Analysis of Microbial Diversity

제당폐수를 이용한 수소생산과 미생물의 군집해석

  • Lee, Heesu (Department of Environmental Engineering Seoul National University of Science & Technology) ;
  • Lee, Tae-Jin (Department of Environmental Engineering Seoul National University of Science & Technology)
  • 이희수 (서울과학기술대학교 환경공학과) ;
  • 이태진 (서울과학기술대학교 환경공학과)
  • Published : 2012.09.30
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Abstract

Biohydrogen production and analysis of microbial community were attempted from the sugar manufacturing wastewater with anaerobic fermentation process. Addtion of nutrients ($N{\cdot}P$) into sugar manufacturing wastewater stimulates hydrogen production from 9.53 to $26.67m{\ell}$ $H_2/g$ COD. Butyric acid, acetic acid, lactic acid, and propionic acid were detected in the sample of the anaerobic fermentation process. Butyric acid/Acetic acid(B/A) ratio was increased 0.50 to 0.92 according to the nutrients addtion into the wastewater. Microbial community was analyzed as Clostridium sp. in the phylum of Firmicutes and Klebsiella sp., Erwinia sp., and enterobacter sp. of the class of $\gamma$-Proteobacteria. As the improvement of hydrogen production, Erwinia sp. was decreased and Klebsiella sp. was increased.

생물학적 혐기발효를 이용하여 제당폐수로부터 바이오 수소가스의 생산 수율을 알아보고, 이 반응에 관여하는 미생물의 군집양상을 살펴보았다. 제당폐수 내 영양염류($N{\cdot}P$)의 공급함에 따라 수소발생량이 9.53 에서 $26.67m{\ell}$ $H_2/g$ COD로 증가하였다. 혐기발효과정에서 butyric acid, acetic acid, lacticacid, 그리고 propionic acid이 검출되었다. Butyric acid/acetic acid(B/A)비는 제당폐수에 영양염류가 공급됨에 따라 0.50에서 0.92로 증가 하였으며, B/A비가 높을수록 수소생산량이 증가하였다. 제당폐수의 발효과정에서 나타나는 미생물 군집은 Firmicutes문에는 Clostridium 속으로 나타났으며 $\gamma$-Proteobacteria 강에는 Klebsiella 속, Erwinia 속, 그리고 Eenterobacter 속이 검출되었다. 또한 수소 생성이 활성화 되면서 Erwinia 속은 위축되고 Klebsiella 속이 많아지는 것을 알 수 있었다.

Keywords

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