Journal of the Korea Organic Resources Recycling Association (유기물자원화)

Korea Organic Resources Recycling Association (유기성자원학회)
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Optimization of bio-$H_{2}$ production from acid pretreated microalgal biomass

미세조류로부터 바이오 수소 생산을 위한 산(acid) 전처리의 최적화

  • Yun, Yeo-Myeong (Department of Civil and Environmental Engineering, KAIST) ;
  • Jung, Kyung-Won (Department of Civil and Environmental Engineering, KAIST) ;
  • Kim, Dong-Hoon (Clean Fuel Center, Korea Institute of Energy Research) ;
  • Oh, You-Kwan (Clean Fuel Center, Korea Institute of Energy Research) ;
  • Shin, Hang-Sik (Department of Civil and Environmental Engineering, KAIST)
  • 윤여명 (한국과학기술원 건설 및 환경공학과) ;
  • 정경원 (한국과학기술원 건설 및 환경공학과) ;
  • 김동훈 (한국에너지기술연구원 청정연료연구단) ;
  • 오유관 (한국에너지기술연구원 청정연료연구단) ;
  • 신항식 (한국과학기술원 건설 및 환경공학과)
  • Published : 2012.03.31
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Abstract

In this study, dark fermentative hydrogen production (DFHP) from acid pretreated microalgal biomass was optimized with via statistical experimental design. Acid concentration and reaction time were varied from 0.1 to 3% (v/w) and 10 to 60 min with substrate concentration of 76 g dry cell weight (dcw)/L and initial pH of 7.4, respectively. During the fermentation, pH was not controlled. The optimal condition was found that at $H_{2}$ yield reached to 37.3 mL $H_{2}/g$ dcw at 1.2% HCl and 48 min. Through regression analysis, it was found that $H_{2}$ yield was well fitted by a quadratic polynomial equation ($R^{2}$=0.95). HCl concentration was the most significant factor influencing DFHP. The results of ANOVA verify that HCl concentration was the most significant factor influencing DFHP.

본 연구에서는 제 3세대 바이오매스 미세조류를 이용한 혐기성 암발효 수소 생산 과정에서 산 전처리의 최적화를 통계학적 실험방법인 반응표면법을 적용하여 도출 하였다. 1~3% (v/w)의 산 농도와 10~60 min 전처리 시간을 최적화 실험 범위로 설정하였으며 기질농도 76 g dcw/L와 초기 pH는 7.4로 고정하였고 수소발효 운전 중에 pH는 조절하지 않았다. 최적화 결과 HCl 1.2%와 반응시간 48 min에서 가장 높은 수소전환율인 36.8 mL $H_{2}/g$ dcw을 얻었으며 이때 가용화율은 18.9%로 나타났다. 정확도는 $R^{2}$=0.95로 매우 정확한 상관계수를 보였고 ANOVA test를 통해 예측된 수소전환율에 관련한 경험식은 a quadratic polynomial equation 으로 나타났으며 반응시간보다 산주입농도가 수소 생산에 큰 영향을 미치는 것으로 나타났다.

Keywords

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