Annals of Laboratory Medicine

Korean Society for Laboratory Medicine (대한진단검사의학회)
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Multiplex PCR for Rapid Detection of Genes Encoding Class A Carbapenemases

  • Hong, Sang Sook (Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University) ;
  • Kim, Kyeongmi (Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University) ;
  • Huh, Ji Young (Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University) ;
  • Jung, Bochan (Department of Laboratory Medicine, CHA Gumi Medical Center, CHA University) ;
  • Kang, Myung Seo (Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University) ;
  • Hong, Seong Geun (Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University)
  • Published : 2012.09.01
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Abstract

In recent years, there have been increasing reports of KPC-producing Klebsiella pneumoniae in Korea. The modified Hodge test can be used as a phenotypic screening test for class A carbapenamase (CAC)-producing clinical isolates; however, it does not distinguish between carbapenemase types. The confirmation of type of CAC is important to ensure optimal therapy and to prevent transmission. This study applied a novel multiplex PCR assay to detect and differentiate CAC genes in a single reaction. Four primer pairs were designed to amplify fragments encoding 4 CAC families (SME, IMI/NMC-A, KPC, and GES). The multiplex PCR detected all genes tested for 4 CAC families that could be differentiated by fragment size according to gene type. This multiplex PCR offers a simple and useful approach for detecting and distinguishing CAC genes in carbapenem-resistant strains that are metallo-$\beta$-lactamase nonproducers.

Keywords

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