Annals of Laboratory Medicine

Korean Society for Laboratory Medicine (대한진단검사의학회)
권호 표지
DOI QR코드

DOI QR Code

AbaR7, a Genomic Resistance Island Found in Multidrug-resistant Acinetobacter baumannii Isolates in Daejeon, Korea

  • Sung, Ji Youn (Department of Biomedical Laboratory Science, Far East University) ;
  • Koo, Sun Hoe (Department of Laboratory Medicine, Chungnam National University College of Medicine) ;
  • Cho, Hye Hyun (Department of Biomedical Laboratory Science, Jeonju Kijeon College) ;
  • Kwon, Kye Chul (Department of Laboratory Medicine, Chungnam National University College of Medicine)
  • Published : 2012.09.01
⟨ Previous Next ⟩

Abstract

Background: Acinetobacter baumannii resistance islands (AbaRs) have been recently recognized as mobile genetic elements that harbor multiple resistance determinants and are associated with multidrug resistance (MDR). In the present study, we aimed to determine the AbaRs conferring multiple antimicrobial resistance and their clonal relatedness to MDR A. baumannii clinical isolates obtained from a university hospital in Daejeon, Korea. Methods: This study included 29 MDR A. baumannii strains isolated in Daejeon, Korea. The minimal inhibitory concentrations (MICs) were determined by Etest. A. baumannii isolates were characterized using the 2 multiplex PCR assays and multilocus sequence typing (MLST) scheme. To detect and characterize AbaRs, PCR and PCR mapping experiments were performed. Results: Twenty-seven of the 29 isolates belonged to the European (EU) clone II lineage and contained 5 sequence types (STs) (75, 92, 137, 138, and 357). In this study, ST357 was confirmed for the first time in Korea. Only 2 of the 29 isolates belonged to the EU clone I lineage, and were confirmed as ST109. These 2 isolates harbored the 22-kb AbaR7 aacC1-orfP-orfQ-aadA1 gene cassette array. In contrast, AbaR was not found in EU clone II isolates. Conclusions: This is the first study that attempted to determine the AbaRs in MDR A. baumannii isolates in Korea. We found 2 EU clone I isolates (ST109) that harbored AbaR7.

Keywords

References

  1. Peleg AY, Seifert H, Paterson DL. Acinetobacter baumannii: emergence of a successful pathogen. Clin Microbiol Rev 2008;21:538-82. https://doi.org/10.1128/CMR.00058-07
  2. Krizova L, Dijkshoorn L, Nemec A. Diversity and evolution of AbaR genomic resistance islands in Acinetobacter baumannii strains of European clone I. Antimicrob Agents Chemother 2011;55:3201-6. https://doi.org/10.1128/AAC.00221-11
  3. Petersen A, Guardabassi L, Dalsgaard A, Olsen JE. Class I integrons containing a dhfrI trimethoprim resistance gene cassette in aquatic Acinetobacter spp. FEMS Microbiol Lett 2000;182:73-6. https://doi.org/10.1111/j.1574-6968.2000.tb08876.x
  4. Post V, White PA, Hall RM. Evolution of AbaR-type genomic resistance islands in multiply antibiotic-resistant Acinetobacter baumannii. J Antimicrob Chemother 2010;65:1162-70. https://doi.org/10.1093/jac/dkq095
  5. Sung JY, Kwon KC, Cho HH, Koo SH. Antimicrobial resistance determinants in imipenem-nonsusceptible Acinetobacter calcoaceticus-baumannii complex isolated in Daejeon, Korea. Korean J Lab Med 2011;31:265-70. https://doi.org/10.3343/kjlm.2011.31.4.265
  6. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. Twentieth informational supplement, M100-S20. Wayne, PA: Clinical and Laboratory Standards Institute, 2010.
  7. Ko KS, Suh JY, Kwon KT, Jung SI, Park KH, Kang CI, et al. High rates of resistance to colistin and polymyxin B in subgroups of Acinetobacter baumannii isolates from Korea. J Antimicrob Chemother 2007;60:1163-7. https://doi.org/10.1093/jac/dkm305
  8. Lee K, Yong D, Jeong SH, Chong Y. Multidrug-resistant Acinetobacter spp.: increasingly problematic nosocomial pathogens. Yonsei Med J 2011;52:879-91. https://doi.org/10.3349/ymj.2011.52.6.879
  9. Turton JF, Gabriel SN, Valderrey C, Kaufmann ME, Pitt TL. Use of sequence- based typing and multiplex PCR to identify clonal lineages of outbreak strains of Acinetobacter baumannii. Clin Microbiol Infect 2007;13:807-15. https://doi.org/10.1111/j.1469-0691.2007.01759.x
  10. Bou G, Cervero G, Dominguez MA, Quereda C, Martinez-Beltran J. PCRbased DNA fingerprinting (REP-PCR, AP-PCR) and pulsed field gel electrophoresis characterization of a nosocomial outbreak caused by imipenem- and meropenem-resistant Acinetobacter baumannii . Clin Microbiol Infect 2000;6:635-43. https://doi.org/10.1046/j.1469-0691.2000.00181.x
  11. Bartual SG, Seifert H, Hippler C, Luzon MA, Wisplinghoff H, Rodriguez-Valera F. Development of a multilocus sequence typing scheme for characterization of clinical isolates of Acinetobacter baumannii. J Clin Microbiol 2005;43:4382-90. https://doi.org/10.1128/JCM.43.9.4382-4390.2005
  12. Higgins PG, Dammhayn C, Hackel M, Seifert H. Global spread of carbapenem-resistant Acinetobacter baumannii. J Antimicrob Chemother 2010;65:233-8. https://doi.org/10.1093/jac/dkp428
  13. Hamidian M and Hall RM. AbaR4 replaces AbaR3 in a carbapenem-resistant Acinetobacter baumannii isolate belonging to global clone 1 from an Australian hospital. J Antimicrob Chemother 2011;66:2484-91. https://doi.org/10.1093/jac/dkr356
  14. Turton JF, Kaufmann ME, Glover J, Coelho JM, Warner M, Pike R, et al. Detection and typing of integrons in epidemic strains of Acinetobacter baumannii found in the United Kingdom. J Clin Microbiol 2005;43:3074-82. https://doi.org/10.1128/JCM.43.7.3074-3082.2005
  15. Adams MD, Goglin K, Molyneaux N, Hujer KM, Lavender H, Jamison JJ, et al. Comparative genome sequence analysis of multidrug-resistant Acinetobacter baumannii. J Bacteriol 2008;190:8053-64. https://doi.org/10.1128/JB.00834-08
  16. Shaikh F, Spence RP, Levi K, Ou HY, Deng Z, Towner KJ, et al. ATPase genes of diverse multidrug-resistant Acinetobacter baumannii isolates frequently harbor integrated DNA. J Antimicrob Chemother 2009;63:260-4.
  17. Mugnier PD, Poirel L, Naas T, Nordmann P. Worldwide dissemination of the $bla_{OXA-23}$ carbapenemase gene of Acinetobacter baumannii. Emerg Infect Dis 2010;16:35-40.

Cited by

  1. Comparative study of different molecular methods for typing of Acinetobacter baumannii clinical isolates from University Hospitals vol.36, pp.5, 2012, https://doi.org/10.1007/s13258-014-0183-z
  2. Bacterial Genome Instability vol.78, pp.1, 2014, https://doi.org/10.1128/mmbr.00035-13
  3. Carbapenem resistance in Acinetobacter baumannii, and their importance in hospital‐acquired infections: a scientific review vol.131, pp.6, 2012, https://doi.org/10.1111/jam.15130