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Escherichia coli O157:H7에 감염된 마우스에 대한 염소산나트륨과 한약재 복합추출물 합제의 항균효과

Antimicrobial Activity of Sodium chlorate and Korean Herbal Extracts against Mice infected with Escherichia coli O157:H7

  • 차춘남 (경상대학교 산업시스템공학부 공학연구원) ;
  • 이여은 (경상대학교 보건대학원 환경보건학과) ;
  • 손송이 (경상대학교 수의과대학 생명과학연구소) ;
  • 박은기 (고신대학교 인문사회의학교실) ;
  • 최현주 (인제대학교 임상병리학과, 고령자라이프디자인연구소) ;
  • 김석 (경상대학교 수의과대학 생명과학연구소) ;
  • 이후장 (경상대학교 수의과대학 생명과학연구소)
  • Cha, Chun-Nam (Engineering Research Institute, Department of Industrial Systems Engineering, Gyeongsang National University) ;
  • Lee, Yeo-Eun (Department of Environmental Health, Graduate School of Public Health, Gyeongsang National University) ;
  • Son, Song-Ee (Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University) ;
  • Park, Eun-Kee (Department of Medical Humanities and Social Medicine, College of Medicine, Kosin University) ;
  • Choi, Hyun-Ju (Elderly Life Redesign Institute, Department of Biomedical Laboratory Science, Inje University) ;
  • Kim, Suk (Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Hu-Jang (Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University)
  • 투고 : 2012.01.15
  • 심사 : 2012.03.15
  • 발행 : 2012.03.31

초록

본 연구는 황련, 감초, 오미자 그리고 산수유 한약재 복합추출물의 $E.$ $coli$ O157:H7에 대한 항균효과와, 한약재 복합추출물과 염소산나트륨 합제의 $E.$ $coli$ O157:H7 감염 마우스에 대한 항균효과를 평가하기 위해 수행되었다. 한약재 복합추출물, CGSC10을 이용하여 $E.$ $coli$ O157:H7에 대한 항균효과 확인 시험을 수행한 결과, CGSC10 5, 10, 그리고 20%를 첨가한 배지에서 30시간 동안 배양한 후, $E.$ $coli$ O157:H7의 성장이 각각 34.7, 60.2, 그리고 76.4% 억제되는 효과가 관찰되었다. 또한, $E.$ $coli$ O157:H7을 감염시킨 마우스에 CGSC10, 염소산나트륨, 그리고 CGSC10와 염소산나트륨 합제(CGSCS10)를 음용수를 통해 투여한 결과, 투여 3일째부터, 모든 투여군에서 대조군과 비교하여 $E.$ $coli$ O157:H7의 균수가 유의성 있게 감소하였으며($p$ < 0.001), 투여 7일째에는, 10% CGSC10, 15mM 염소산나트륨, 그리고 CGSCS10을 투여한 군에서 대조군과 비교하여 $E.$ $coli$ O157:H7의 균수가 각각 27.1, 67.7, 그리고 83.3% 정도 감소하는 결과를 나타내었다. 따라서 본 연구의 결과로부터, CGSCS10을 $E.$ $coli$ O157:H7에 감염된 마우스에 음수로 투여할 경우, 감염증상을 완화 시킬 수 있을 것으로 기대된다.

The present study was evaluated the antibacterial effect of the combination of $Coptidis$ $rhizoma$, $Glycyrrhiza$ $uralensis$ Fischet, $Schizandra$ $chinensis$ and $Corni$ $Fructus$(1:1:1) extracts(CGSC10). Furthermore, the effectiveness of CGSC10, sodium chlorate, and the combination of CGSC10 and sodium chlorate(CGSCS10) against $E.$ $coli$ O157:H7 infection was studied using ICR female mice. During the incubation period, the dose of 5, 10, and 20% CGSC10 was inhibited the growth of $E.$ $coli$ O157:H7 by 34.7, 60.2, and 76.4%, respectively. For 7 days after single challenge with $E.$ $coli$ O157:H7, forty female ICR mice were divided into four experimental groups which were administered in drinking water with saline, 10% CGSC10, 15 mM sodium chlorate, and CGSCS10, respectively. On the 3rd day, the number of $E.$ $coli$ O157:H7 in mouse feces was significantly decreased by administration of CGSC10, 15 mM sodium chlorate, and CGSCS10 ($p$ < 0.001). On the 7th day-after administration, CGSC10, sodium chlorate, and CGSCS10 were decreased the number of $E.$ $coli$ O157:H7 by 27.1, 67.7, and 83.3%, respectively. According to the results of the present study, administration of CGSCS10 to mice can reduce the severity of $E.$ $coli$ O157:H7 infection. In addition, it is suggested that CGSCS10 represents a good candidate for the treatment of enteric infections in domestic animals.

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피인용 문헌

  1. Antibacterial Activity of Aqueous Garlic Extract Against Escherichia coli O157:H7, Salmonella typhimurium and Staphylococcus aureus vol.30, pp.2, 2015, https://doi.org/10.13103/JFHS.2015.30.2.210