생명과학회지 (Journal of Life Science)

  • 제22권5호
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  • Pages.687-691
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  • 2012
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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성숙마우스의 난소에서 황체형성호르몬수용체와 galectin-3의 상반된 면역조직화학적 발현분포

Opposite Localization of Luteinizing Hormone Receptors and Galectin-3 in Mature Mouse Ovaries

  • Kim, Ju-Hwan (College of Veterinary Medicine and Animal Medical Institute, Chonnam National University) ;
  • Yang, Mi-Young (College of Veterinary Medicine and Animal Medical Institute, Chonnam National University) ;
  • Kim, Sung-Ho (College of Veterinary Medicine and Animal Medical Institute, Chonnam National University) ;
  • Kim, Jong-Choon (College of Veterinary Medicine and Animal Medical Institute, Chonnam National University) ;
  • Kim, Seung-Joon (College of Veterinary Medicine, Kyungpook National University) ;
  • Joo, Hong-Gu (College of Veterinary Medicine, Jeju National University) ;
  • Shin, Tae-Kyun (College of Veterinary Medicine, Jeju National University) ;
  • Moon, Chang-Jong (College of Veterinary Medicine and Animal Medical Institute, Chonnam National University)
  • 투고 : 2012.01.05
  • 심사 : 2012.05.08
  • 발행 : 2012.05.30
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초록

본 연구는 성숙마우스의 난소에서 황체형성호르몬수용체(luteinizing hormone receptor, LHR)와 beta-galctoside-binding animal lectin인 galetin-3 (Gal-3)의 발현분포를 면역조직화학방법으로 평가하였다. LHR은 활동(성숙) 황체에서 강하게 발현되는 반면에, Gal-3의 발현은 퇴행중인 황체와 폐쇄난포에서 높게 나타났다. 또한, 임신중인 마우스의 황체에서는 LHR의 면역반응이 강하게 나타났으나, Gal-3의 면역반응은 현저히 낮았다. 위의 결과를 종합해 볼 때, 성숙마우스의 난소에서 LHR과 Gal-3는 난소의 특정한 시기에 따라 다른 분포양상을 나타냄을 알 수 있었으며, 이들 각각의 단백질의 발현으로 보아 황체의 형성과 퇴행에서 상반된 역할을 할 것으로 여겨진다.

The present study evaluated the localization of luteinizing hormone receptors (LHR) and galectin-3 (Gal-3), a beta-galactoside-binding animal lectin, in the mature mouse ovaries by immunohistochemical analysis. Intense LHR immunoreactivity was detected in the active corpus luteum (CL), whereas expression of Gal-3 was high in the regressing CL and atretic follicle. In the CL of pregnant mice, LHR immunoreactivity was intense, but Gal-3 expression was low. Thus, LHR and Gal-3 had opposite patterns of expression in mature mouse ovaries, suggesting that both proteins have stage-specific expression patterns and are possibly involved in CL formation and regression.

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참고문헌

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피인용 문헌

  1. Unilaminar follicular cells transiently express galectin-3 during ovarian folliculogenesis in pigs vol.49, pp.1, 2017, https://doi.org/10.1016/j.tice.2016.11.003