생명과학회지 (Journal of Life Science)

  • 제22권5호
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  • Pages.627-633
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  • 2012
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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해양미생물 Microbulbifer sp. AJ-3을 이용한 매생이 효소분해산물의 생리활성 연구

Biological Analysis of Enzymatic Extracts from Capsosiphon Fulvescens Using the Microbulbifer sp. AJ-3 Marine Bacterium

  • 배정미 (신라대학교 식품영양학과) ;
  • 조은경 (신라대학교 식품영양학과) ;
  • 김혜윤 (신라대학교 식품영양학과) ;
  • 강수희 (신라대학교 식품영양학과) ;
  • 최영주 (신라대학교 식품영양학과)
  • Bae, Jeong-Mi (Department of Food and Nutrition, College of Medical Life Sciences, Silla University) ;
  • Cho, Eun-Kyung (Department of Food and Nutrition, College of Medical Life Sciences, Silla University) ;
  • Kim, Hye-Youn (Department of Food and Nutrition, College of Medical Life Sciences, Silla University) ;
  • Kang, Su-Hee (Department of Food and Nutrition, College of Medical Life Sciences, Silla University) ;
  • Choi, Young-Ju (Department of Food and Nutrition, College of Medical Life Sciences, Silla University)
  • 투고 : 2012.02.24
  • 심사 : 2012.05.22
  • 발행 : 2012.05.30
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초록

본 연구에서는 해수로부터 난소화성 복합다당류를 분해하는 해양미생물 $Microbulbifer$ sp. AJ-3의 효소에 대한 기질 특이성을 분석하고 균주가 생산하는 효소를 이용하여 매생이의 효소분해산물을 조제하여 이에대한 생리활성을 분석하였다. 효소의 활성은 agar에 대한 상대적인 활성으로 나타내었는데 chitin 34.8%, fucoidan 36.8%로 비교적 낮은 활성을 보였고, starch 51.2%, agarose와 laminaran은 agar와 비슷한 수준인 115.6%, 103.1%의 활성을 나타내었다. 이 뿐만 아니라 alginic acid에 대해서는 agar의 2배가 넘는 239.3%의 활성을 나타내었다. 매생이 효소분해산물의 항산화능은 DPPH radical 소거능과 SOD 활성측정으로 분석하였는데 매생이 효소분해산물 2 mg/ml의 농도에서 DPPH 라디칼 소거능은 32%, SOD 활성은 93%로 나타났다. 매생이 효소분해산물의 아질산염 소거능은 pH 1.2에서 82%, pH 3.0에서 53%, pH 6.0에서 12%로 positive control인 비타민C와 유사한 활성을 보였다. 매생이 효소분해산물의 숙취해소 효능은 ADH 활성증진에 미치는 영향을 조사하였는데 효소분해산물 2 mg/ml 농도에서 30%의 증가된 알콜 분해능이 나타났다. 매생이 효소분해산물의 미백 효능에 대해서는 tyrosinase 저해능으로 분석하였으며 2 mg/ml에서 28%의 저해활성을 나타내었고 농도가 증가함에 따라 유의적으로 증가하는 경향을 나타내었다.

$Microbulbifer$ sp. AJ-3 was used to acquire the degrading products from $Capsosiphon$ $fulvescens$ (DPCF), which were investigated to determine its physiological activities. A crude enzyme extract from $Microbulbifer$ sp. AJ-3 hydrolyzes polysaccharide substrates such as agar, agarose, alginic acid, fucoidan, laminaran, starch, and chitin. Among them, agarose, laminaran, and alginic acid showed higher activities, especially alginic acid. The antioxidant activity of DPCF was measured by using 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and superoxide dismutase (SOD)-like activities and were about 32% and 93% at 2 mg/ml, respectively. In addition, the nitrite-scavenging activity of DPCF was about 82%, 53%, and 12% at pH levels of 1.2, 3.0, and 6.0, respectively. To determine the influence of DPCF on alcohol metabolism, the generating activity of reduced-nicotinamide adenine dinucleotide (NADH) by alcohol dehydrogenase (ADH) was measured. The facilitating rate of ADH activity by DPCF was 130% at 2 mg/ml. The tyrosinase inhibitory activity of DPCF was slightly increased in a dose-dependent manner and was about 28% at 2 mg/ml. These results indicated that the enzymatic products from DPCF have a strong antioxidant, nitrite scavenging, and alcohol metabolizing activity.

키워드

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피인용 문헌

  1. α-Glucosidase, Tyrosinase, and Elastase Inhibitory Effects of Enzymatic Extracts from Ecklonia cava and its Alcohol Metabolizing Activity vol.22, pp.6, 2012, https://doi.org/10.5352/JLS.2012.22.6.751
  2. Studies on Bioactive Substances and Antioxidant Activities of Marine Algae from Jeju Island vol.8, pp.1, 2016, https://doi.org/10.15433/ksmb.2016.8.1.030
  3. Whitening Effect of Green Tea Seed Shell Ethanol Extracts vol.44, pp.10, 2015, https://doi.org/10.3746/jkfn.2015.44.10.1470