Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of Sopung-tang and tDCS on Motor function Recovery and GDNF Expression in Photothrombotic Brain Infarction Induced Rat Model

광혈전 유도 뇌손상 백서에서 소풍탕(疏風湯)과 tDCS의 적용이 운동기능회복과 GDNF 발현에 미치는 영향

  • Sim, Ki Cheol (Chung-yeon Oriental Medicine hospital) ;
  • Kim, Gi Do (Department of Physical Therapy, International University of Korea) ;
  • Kim, Kyung Yoon (Department of Physical Therapy, Dongshin University) ;
  • Chung, Hun Woo (Department of Oriental Medicine of Dongshin University) ;
  • Kim, Gye Yeop (Department of Physical Therapy, Dongshin University)
  • Received : 2012.07.05
  • Accepted : 2012.12.12
  • Published : 2012.12.25
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Abstract

The effect of Sopung-tang(SPT) and trans-cranial direct current stimulation(tDCS) was investigated in photothrombotic brain infarction(PTI) rats. Sprague-Dawley 80 rats, were divided into four groups. group I was experiental control group(n=20), group II was PTI induced and oral administration of SPT(n=20), group III was PTI induced and tDCS administration(n=20) and group IV was PTI induced and SPT and tDCS administration for 28 days on stroke rats(n=20). Analysis the neurological function test, 25 point behavior functional score test, and immunohistochemistric finding of GDNF expression, and electron microscopy assessment In motor behavior test, the outcome of group IV was significantly difference than the other group, and In immunohistochemistric finding, group II, III, IV were increase GDNF expression on 28 days, In electron microscopy finding, the all groups were degenerated of cell organelles, and synaptic plasticity were improvement of group II, III, IV(especially group IV) These results suggest that, 28days application of SPT and tDCS was the motor function and histopathologic, micro-morphological improvement of motor function recovery and positive influence on synaptic plasticity.

Keywords

Acknowledgement

Supported by : 동신대학교

References

  1. 전국한의과대학심계내과교실. 심계내과학. 서울, 군자출판사, p 331, 1996.
  2. Kim, M.S., Shin, Y.I., Kim, H.I., Moon, S.K., Lee, S., Moon, B.S., Lee, M.C. Relevance of behavioral test in the photothrombotic stroke rat model. J of Korean Acad of Rehab Med. 30(2):135-141, 2006.
  3. Sigler, A., Goroshkov, A., Murphy, T.H. Hardware and methodology for targeting single brain arterioles for photothrombotic stroke on an upright microscope. Journal Neuroscience Methods. 170(1):35-44, 2008. https://doi.org/10.1016/j.jneumeth.2007.12.015
  4. Yuh, W.T.C., Ueda, T., White, M., Schuster, M.E., Taoka, T. The need for objective assessment of the new imaging techniques and understanding the expanding roles of stroke imaging. AJNR. 20: 1779-1784, 1999.
  5. Fisher, M. The ischemic penumbra: identification, evolution and treatment concept. Cerebrovasc Dis. 17(1):1-6, 2004. https://doi.org/10.1159/000073891
  6. Langhorne, P., Coupar, F., Pollock, A. Motor recovery after stroke: a systematic review. Lancet Neurol. 8: 741-754, 2009. https://doi.org/10.1016/S1474-4422(09)70150-4
  7. Edwards, M.T., Murphy, M.M., Geraghty, J.J., Wulf, J.A., Konzen, J.P. Intra-arterial cerebral thrombolysis for acute ischemic stroke in a community hospital. AJNR Am J Neuroradiol. 20(9):1682-1687, 1999.
  8. 공정현. 萬病回春. 서울, 행림서원, pp 55-57, 1975.
  9. 허준. 동의보감. 서울, 동의보감, p 992, 2005.
  10. 정장균. 疏風湯이 실험동물의 심혈관계에 미치는 영향. 동의병리학회지 10: 317-340, 1995.
  11. 문영희, 정명현, 주홍규, 임동윤, 유호진. 疏風湯이 흰쥐의 혈압에 미치는 영향. 생약학회지 21(2):173-178, 1990.
  12. 정장균, 안일회. 疏風湯이 뇌혈전증 초기환자에 미치는 영향. 원광한의학. 5: 181-195, 1995.
  13. 추민규, 최진봉, 신미숙, 김선종. 疏風湯이 허혈성 뇌손상흰쥐의 인지 및 운동기능 회복에 미치는 영향. 한방재활의학과학회지 18(2):45-60, 2008.
  14. 권영철, 이경섭. 疏風湯및 加味疏風湯이 고지혈증에 미치는 영향. 경희한의대논문집. 5: 269-279, 1982.
  15. Nitsche, M.A., Cohen, L.G., Wassermann, E.M., Priori, A., Lang, N., Antal, A., Paulus, W., Hummel, F., Boggio, P.S., Fregni, F., Pascual-Leone, A. Transcranial direct current stimulation: State of the art 2008. Brain Stimul. 1(3):206-223, 2008. https://doi.org/10.1016/j.brs.2008.06.004
  16. Fregni, F., Boggio, P.S., Nitsche, M., Bermpohl, F., Antal, A., Feredoes, E., Marcolin, M.A., Rigonatti, S.P., Silva, M.T., Paulus, W., Pascual-Leone A. Anodal transcranial direct current stimulation of prefrontal cortex enhances working memory. Exp Brain Res. 166(1):23-30, 2005. https://doi.org/10.1007/s00221-005-2334-6
  17. Antal, A., Varga, E.T., Kincses, T.Z., Nitsche, M.A. Paulus, W. Oscillatory brain activity and transcranial direct current stimulation in humans. Neuroreport. 15(8):1307-1310, 2004. https://doi.org/10.1097/01.wnr.0000127460.08361.84
  18. Hummel, F., Celnik, P., Giraux, P., Floel, A., Wu, W.H., Gerloff, C., Cohen, L.G. Effects of non-invasive cortical stimulation on skilled motor function in chronic stroke. Brain. 128(3):490-499, 2005. https://doi.org/10.1093/brain/awh369
  19. Beck, K.D., Valverde, J., Alexi, T., Poulsen, K., Moffat B., Vandlen R.A., Rosenthal, A,. Hefti, F. Mesencephalic dopaminergic neurons protected by GDNF from axotomy-induced degeneration in the adult brain. Nature. 373(6512):339-341, 1995. https://doi.org/10.1038/373339a0
  20. Sauer, H., Rosenblad, C., Bjorklund, A. Glial cell line-derived neurotrophic factor but not transforming growth factor beta 3 prevents delayed degeneration of nigral dopaminergic neurons following striatal 6-hydroxydopamine lesion. Proc Natl Acad Sci USA. 92(19):8935-8939, 1995. https://doi.org/10.1073/pnas.92.19.8935
  21. Ito, M. Mechanisms of motor learning in the cerebellum. Brain Res. 886(1-2):237-245, 2000. https://doi.org/10.1016/S0006-8993(00)03142-5
  22. Sanes, J.N. Neocortical mechanisms in motor learning. Curr Opin Neurobiol. 13(2):225-231, 2003. https://doi.org/10.1016/S0959-4388(03)00046-1
  23. Rema, V., Ebner, F.F. Effect of enriched environment rearing on impairments in cortical excitability and plasticity after prenatal alcohol exposure. J Neurosci. 19(24):10993-11006, 1999. https://doi.org/10.1523/JNEUROSCI.19-24-10993.1999
  24. 최서우. 소풍탕이 고지혈증 흰쥐의 뇌경색에 미치는 영향. 경원대학교 대학원 박사학위논문, 2008.
  25. Paxinos, G., Watson, C. The rat brain in stereotactic coordinates. 5th ed Sydney Academic press. 9: 203, 2004.
  26. Watson, B.D., Dietrich, W.D., Busto, R., Wachtel, M.S., Ginsberg, M.D. Induction of reproducible brain infarction by photochemically initiated thrombosis. Ann Neurol. 17: 497-504, 1985. https://doi.org/10.1002/ana.410170513
  27. 김상준. 중풍 백서에서 반복적 경두개 직류 전기 자극에 의한 운동 기능 회복 기전. 서울대학교 대학원 박사학위논문, 2009.
  28. Bederson, J.B., Germano, I.M., Guarino, L. Cortical blood flow and cerebral perfusion pressure in a new noncraniotomy model of subarachnoid hemorrhage in the rat. Stroke. 26: 1086-1091, 1995. https://doi.org/10.1161/01.STR.26.6.1086
  29. Feldman, Z., Gurevitch, B., Artru, A.A., Oppenheim, A., Shohami, E., Reichenthal, E., Shapira, Y. Effect of magnesium given 1 hour after head trauma on brain edema and neurological outcome. J Neurosurg. 85: 131-137, 1996. https://doi.org/10.3171/jns.1996.85.1.0131
  30. Paulus, W. Transcranial direct current stimulation (tDCS). Suppl Clin Neurophysiol. 56: 249-254, 2003.
  31. Wassermann E.M., Grafman J. Recharging cognition with DC brain polarization. Trends Cogn Sci. 9(11):503-505, 2005. https://doi.org/10.1016/j.tics.2005.09.001
  32. Kumiko, T., Atsushi, S., Hideki, H., Takashi, S., Hidenori, S. Overexpression of GDNF in the Uninjured DRG Exerts Analgesic Effects on Neuropathic Pain Following Segmental Spinal Nerve Ligation in Mice. The Journal of Pain. 12(11):1130-1139, 2011. https://doi.org/10.1016/j.jpain.2011.04.003
  33. Carmichael, S.T. Cellular and molecular mechanisms of neural repair after stroke: making waves. Annals of Neurology. 59(5):735-742, 2006. https://doi.org/10.1002/ana.20845