Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of Ukgan-san on the L-thyroxine-induced Rat Hyperthyroidism

억간산이 갑상선기능항진 유발 백서에 미치는 영향

  • Kim, Seung Mo (College of Oriental Medicine, Daegu Haany University)
  • 김승모 (대구한의대학교 한의과대학)
  • Received : 2012.07.25
  • Accepted : 2012.10.18
  • Published : 2012.10.25
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Abstract

This study was to elucidate the effects of Ukgan-san on the hyperthyroidism induced by sodium levothyroxine. Hyperthyroidisms were induced by continuous subcutaneous treatment of LT4, 0.3 mg/kg, once a day for 27days, and 1,500, 1,000 and 500 mg/kg of Ulkansan extracts were orally administered, once a day for 15 days from 12th LT4 treatment, and the changes on the body, thyroid gland, liver and epididymal fat pad weights, serum triiodothyronine(T3), thyroxine(T4), thyroid-stimulating hormone(TSH), asparte aminotransferase (AST) and alanine aminotransferase (ALT) concentrations, hepatic lipid peroxidation(LPO), glutathione(GSH), superoxide dismutase(SOD) and catalase(CAT) activities were investigated with throid gland, liver and epididymal fat pad histopathological changes. The effects of Ukan-san extracts were compared with that of propyl thiouracil(PTU), a standard antithyroidic drug 10 mg/kg(intraperitoneally). 1,500 and 1,000 mg/kg of Ukan-san extracts reversed all LT4-induced hyperthyroidisms and these effects indicating their potential in the regulation of hyperthyroidism. Further, the Ukan-san extract normalized LT4-induced liver oxidative stresses, and also reduced liver damages and epididymal fat pad reducements suggesting its antioxidative and relative organ protective nature. However, nor favorable effects on LT4-induced hyperthyroidisms were detected in Ulkansan 500 mg/kg treated rats as compared with LT4 control rats in the present study. These effects of Ukan-san may help improvement of hyperthyroidisms and accompanied various organ damages.

Keywords

Acknowledgement

Supported by : 대구한의대학교

References

  1. Gartner, L.P., Hiatt, J.L. Color textbook of histology. Philadelphia, Saunders, pp 303-325, 2007.
  2. Capen, C.C., Martin, S.L. The thyroid gland. In: McDonald LE and Pineda MH (Eds). Veterinary endocrinology and reproduction, Philadelphia, Lea & Febiger, pp 58-91, 1989.
  3. Cho, C.S., Choi, H.S., Kim, C.J. The Effects of YUKWOOLTANG on the Hyperthyroidism of Rats. The Korea journal of herbology. 21(4):11-17, 2006.
  4. Cho, C.S., Kim, M.S., Kim, C.J. The Effects of KABHANBANG(KHB) on the Hyperthyroidism of Rats. The Korea journal of herbology. 21(4):19-25, 2006.
  5. Chen Guiyan, Yangsi Shu. Chinese Integrative diagnostic Therapy. Seoul, IlJung Publishing press, pp 705-708, 1992,
  6. Panda, S., Kar, A. Amelioration of L-thyroxine-induced hyperthyroidism by coumarin (1,2-benzopyrone) in female rats. Clin Exp Pharmacol Physiol. 34: 1217-1219, 2007.
  7. Venditti, P., Balestrieri, M., Di Meo, S., De Leo, T. Effect of thyroid state on lipid peroxidation, antioxidant defences, and susceptibility to oxidative stress in rat tissues. J Endocrinol. 155: 151-157, 1997. https://doi.org/10.1677/joe.0.1550151
  8. Das, K., Chainy, G.B. Modulation of rat liver mitochondrial antioxidant defence system by thyroid hormone. Biochim Biophys Acta. 1537: 1-13, 2001. https://doi.org/10.1016/S0925-4439(01)00048-5
  9. Majima, T., Komatsu, Y., Doi, K., Takagi, C., Shigemoto, M., Fukao, A., Morimoto, T., Corners, J., Nakao, K. Clinical significance of risedronate for osteoporosis in the initial treatment of male patients with Graves' disease. J Bone Miner Metab. 24: 105-113, 2006. https://doi.org/10.1007/s00774-005-0655-y
  10. Roffi, M., Cattaneo, F., Brandle, M. Thyrotoxicosis and the cardiovascular system. Minerva Endocrinol. 30: 47-58, 2005.
  11. Simon-Giavarotti, K.A., Giavarotti, L., Gomes, L.F., Lima, A.F., Veridiano, A.M., Garcia, E.A., Mora, O.A., Fernandez, V., Videla, L.A., Junqueira, V.B. Enhancement of lindane-induced liver oxidative stress and hepatotoxicity by thyroid hormone is reduced by gadolinium chloride. Free Radic Res. 36: 1033-1039, 2002. https://doi.org/10.1080/1071576021000028280
  12. Janson, A., Karlsson, F.A., Micha-Johansson, G., Bolme, P., Bronnegard, M., Marcus, C. Effects of stimulatory and inhibitory thyrotropin receptor antibodies on lipolysis in infant adipocytes. J Clin Endocrinol Metab. 80: 1712-1716, 1995. https://doi.org/10.1210/jc.80.5.1712
  13. Nakamura, H., Noh, J.Y., Itoh, K., Fukata, S., Miyauchi, A., Hamada, N. Comparison of methimazole and propylthiouracil in patients with hyperthyroidism caused by Graves' disease. J Clin Endocrinol Metab. 92: 2157-2162, 2007. https://doi.org/10.1210/jc.2006-2135
  14. Bahn, R.S., Burch, H.S., Cooper, D.S., Garber, J.R., Greenlee, C.M., Klein, I.L., Laurberg, P., McDougall, I.R., Rivkees, S.A., Ross, D., Sosa, J.A., Stan, M.N. The role of propylthiouracil in the management of Graves' disease in adults: report of a meeting jointly sponsored by the American thyroid association and the food and drug administration. Thyroid. 19: 673-674, 2009. https://doi.org/10.1089/thy.2009.0169
  15. 薜己. 保嬰攝要. 上海, 上海古籍出版社, p 95, 1991.
  16. 黃義完. 東醫精神科學. 서울, 現代醫學書籍社, pp 608-892, 1987.
  17. Hyun, W.C., Lee, S.R. Studies on the Effect of AntiConvulsion and Analgesic by Ukgan-san and Ukgan-sangami. Journal of oriental neuropsychiatry. 5: 69-79, 1994.
  18. Kim, K.S., Sung, G.K., Moon, B.S. An Experimental Study on the Antiepileptic Effects of Ukgan-san. Journal of korean oriental internal medicine. 19: 57-72, 1998.
  19. Kang, S.Y., Kim, Y.C. Decursinol and decursin protect primary cultured rat cortical cells from glutamate-induced neurotoxicity. J Pharm Pharmacol. 59: 863-870, 2007. https://doi.org/10.1211/jpp.59.6.0013
  20. Yang, W.J., Li, D.P., Li, J.K., Li, M.H., Chen, Y.L., Zhang, P.Z. Synergistic antioxidant activities of eight traditional Chinese herb pairs. Biol Pharm Bull. 32: 1021-1026, 2009. https://doi.org/10.1248/bpb.32.1021
  21. Park, Y.H., Son, I.H., Kim, B., Lyu, Y.S., Moon, H.I., Kang, H.W. Poria cocos water extract (PCW) protects PC12 neuronal cells from beta-amyloid-induced cell death through antioxidant and antiapoptotic functions. Pharmazie. 64: 760-764, 2009.
  22. Yokoyama, K., Shimada, Y., Hori, E., Nakagawa, T., Takagi, S., Sekiya, N., Kouta, K., Nishijo, H., Yokozawa, T., Terasawa, K. Effects of Choto-san and hooks and stems of Uncaria sinensis on antioxidant enzyme activities in the gerbil brain after transient forebrain ischemia. J Ethnopharmacol. 95: 335-343, 2004. https://doi.org/10.1016/j.jep.2004.08.018
  23. 한영선, 박재현. 갑상선기능항진증 및 저하증에 대한 양한방적 고찰. 동서의학, 22(3):17-36, 1997.
  24. Hattori, T., Ito, M., Suzuki, Y. Studies on antinephritic effects of plant components in rats (1). Effects of saikosaponins original-type anti-GBM nephritis in rats and its mechanisms. Nippon Yakurigaku Zasshi. 97: 13-21, 1991. https://doi.org/10.1254/fpj.97.1_13
  25. Lee, Y.S., Kim, S.H., Kim, J.K., Shin, H.K., Kang, Y.H., Park, J.H., Lim, S.S. Rapid identification and preparative isolation of antioxidant components in licorice. J Sep Sci. 33: 664-671, 2010. https://doi.org/10.1002/jssc.200900620
  26. Ma, Y.Q., Ye, X.Q., Fang, Z.X., Chen, J.C., Xu, G.H., Liu, D.H. Phenolic compounds and antioxidant activity of extracts from ultrasonic treatment of Satsuma Mandarin (Citrus unshiu Marc.) peels. J Agric Food Chem. 56: 5682-5690, 2008. https://doi.org/10.1021/jf072474o
  27. Han, M.H., Yang, X.W., Zhong, G.Y., Zhang, M. Bioactive constituents inhibiting TNF-alpha production in fresh rhizome of Pinellia ternata. Zhongguo Zhong Yao Za Zhi. 32: 1755-1759, 2007.
  28. Kim, S.M. Effects of ginseng radix on the rat hypothyroidism induced by PTU (6-n-propyl-2-thiouracil). Kor J Herbology. 25: 11-18, 2010.
  29. Subudhi, U., Das, K., Paital, B., Bhanja, S., Chainy, G.B. Alleviation of enhanced oxidative stress and oxygen consumption of L-thyroxine induced hyperthyroid rat liver mitochondria by vitamin E and curcumin. Chem Biol Interact. 173: 105-114, 2008. https://doi.org/10.1016/j.cbi.2008.02.005
  30. Lowry, O.H., Rosenbrough, N.J., Farr, A.L., Randall, R.J. Protein measurement with the Folin phenol reagent. J Biol Chem. 193: 265-275. 1951
  31. Jamall, I.S., Smith, J.C. Effects of cadmium on glutathione peroxidase, superoxidase dismutase and lipid peroxidation in the rat heart: a possible mechanism of cadmium cardiotoxicity. Toxicol Appl Pharmacol. 80: 33-42, 1985. https://doi.org/10.1016/0041-008X(85)90098-5
  32. Sedlak, J., Lindsay, R.H. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem. 25: 192-205, 1968. https://doi.org/10.1016/0003-2697(68)90092-4
  33. Aebi, H. Catalase. In: Bergmeyer HU (Ed.), Methods in Enzymatic Analysis. New York, Academic Press Inc., pp 673-686, 1974.
  34. Nishikimi, M., Rao, N.A., Yagi, K. The occurrence of superoxide anion in the reaction of reduced PMS and molecular oxygen. Biochem Biophys Res Comm. 46: 849-854, 1972. https://doi.org/10.1016/S0006-291X(72)80218-3
  35. Kakkar, P., Das, B., Viswanathan, P.N. Modified spectrophotometric assay of SOD. Indian J Biochem Biophys. 2: 130-132, 1984.
  36. Levene, A. Pathological factors influencing excision of tumours in the head and neck. Part I. Clin Otalary. 6: 145-151, 1981. https://doi.org/10.1111/j.1365-2273.1981.tb01800.x
  37. Chattopadhyay, S., Sahoo, D.K., Subudhi, U., Chainy, G.B. Differential expression profiles of antioxidant enzymes and glutathione redox status in hyperthyroid rats: a temporal analysis. Comp Biochem Physiol C Toxicol Pharmacol. 146: 383-391, 2007. https://doi.org/10.1016/j.cbpc.2007.04.010
  38. Videla, L.A. Energy metabolism, thyroid calorigenesis, and oxidative stress: functional and cytotoxic consequences. Redox Rep. 5: 265-275, 2000. https://doi.org/10.1179/135100000101535807
  39. Messarah, M., Boumendjel, A., Chouabia, A., Klibet, F., Abdennour, C., Boulakoud, M.S., Feki, A.E. Influence of thyroid dysfunction on liver lipid peroxidation and antioxidant status in experimental rats. Exp Toxicol Pathol. 62: 301-310, 2010. https://doi.org/10.1016/j.etp.2009.04.009
  40. Baydasm B., Meral, I. Effects of melatonin on lipid peroxidation and anti-oxidant enzyme activity in rats with experimentally induced hyperthyroidism. Clin Exp Pharmacol Physiol. 32: 541-544, 2005. https://doi.org/10.1111/j.1440-1681.2005.04228.x