Journal of Society of Preventive Korean Medicine (대한예방한의학회지)

Society of Preventive Korean Medicine (대한예방한의학회)
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The anti-cancer effects of $Ampelopsisradix$ Extract (AE) on A549 cells - The role of Bcl-2 family protein on the AE-induced apoptosis -

폐암세포에서 백렴의 항암효능연구 - Bcl-2 family 단백조절을 통한 자가사멸 -

  • Nam, Hye-Seon (Department of Pharmacology, College of Oriental Medicine, Dongguk University) ;
  • Cho, Min-Kyung (Department of Pharmacology, College of Oriental Medicine, Dongguk University)
  • 남혜선 (동국대학교 한의과대학 약리학교실) ;
  • 조민경 (동국대학교 한의과대학 약리학교실)
  • Received : 2012.01.20
  • Accepted : 2012.04.10
  • Published : 2012.04.30
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Abstract

Objective : The aim of this study is to evaluate anti-cancer effects of $Ampelopsisradix$ Extract (AE) on human lung cancer A549 cells. Method : The apoptotic activities and cell growth arrest activities of AE were measured using 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. The molecules involved in apoptotic process were assessed by western blotting. Result : Treatment of AE potently reduced cell viability in a dose-dependent manner in A549 cells. AE (100-500 ${\mu}g/m{\ell}$) resulted in apoptosis via activation of caspase 9 following PARP cleavage in a time-and dose-dependent manner. The levels of Bax and Bad levels were increased by AE with a concomitant decrease of Bcl-xL. In addition, AE at the low dose (30 ${\mu}g/m{\ell}$) significantly inhibited cell growth in the presence of serum. Conclusion : AE has the potential as a therapeutic agent against lung cancer.

Keywords

References

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