칡한우 정액 동결에 있어서 희석액과 수소의 연령이 정자의 동결성, 체외수정란 발달 및 인공수정 임신율에 미치는 효과

Effect of Freezing Buffers and Age of Bulls on Freezability of Semen, $In$ $Vitro$ Embryo Development and the Pregnancy Rate after Artificial Insemination of Korean Native Stripped Bull

  • Park, Yong-Soo (Gyoengbuk Livestock Research Institute) ;
  • Jang, Jong-Sik (Department of Animal Science & Biotechnology, Kyungpook National University)
  • 투고 : 2012.01.15
  • 심사 : 2012.02.05
  • 발행 : 2012.03.31

초록

The purpose of this study was to examine the effect of growing stages of the Korean Native Striped Bull (KNSB) on the freezability and fertility of frozen-thawed semen. First, we investigated the total motility (TM) and progressive motility (PM) according to the diluent used for semen freezing. Second, we examined the effect of the age of KNSB on semen volume, TM and PM of fresh and frozen-thawed semen. Third, we examined the effect of frozen semen from the different age of KNSB on the $in-vitro$ fertilization rate, and the artificial insemination pregnancy rate. The diluents used in this experiment were Triladyl$^{(R)}$ and Tris-egg yolk extender (EYE). Semen was collected from 5 KNSB in the growing stage (15 months) and 5 adult KNSB (36 months). When Triladyl or Tris-EYE extender was used for semen freezing, there was no difference of the mean TM and the mean PM. However, the mean TM was significantly higher in Bull No. 1885 than Bull No. 4283 ($p$ <0.05). The mean volume of semen collected from the 15-month-old bulls (2.3 ml) was significantly lower ($p$ <0.05) than that from the 36-month-old bulls (5.0 ml). The mean semen concentration was similar for the 15-month-old ($2.1{\times}10^9$ spermatozoa/ml) and 36-month-old ($1.8{\times}10^9$ spermatozoa/ml) bulls. For the 15-month-old and 36-month-old bulls, the mean TM of fresh semen were 93.7% and 88.3%, respectively, and the mean PM were 97.0% and 88.3%, respectively; the 15-month-old bulls showed a particularly high PM ($p$ <0.05). For the 15-month-old and 36-month-old bulls, the mean TM (56.0% and 58.0%, respectively) and the mean PM (64.0% and 70.7%, respectively) of frozen-thawed semen did not differ. The development rates of embryos after $in-vitro$ fertilization and the pregnancy rate after artificial insemination using frozen-thawed semen did not differ according to the bull's age. In summary, semen volume differed according to the bull's age, but semen concentration and survival rate, the $in-vitro$ fertilization rate, and the pregnancy rate did not differ according to the stripe bull's age. Accordingly, semen from bulls in the growing stage can be collected and frozen for the preservation and multiplication of rare livestock.

키워드

참고문헌

  1. Abe H, Yamashita S, Satoh T and Hoshi H. 2002. Accumulation of cytoplasmic lipid droplets in bovine embryos and cryotolerance of embryos developed in different culture systems using serum-free or serum containing media. Mol. Reprod. Dev. 61:57-66.
  2. Amann RP, Kavanaugh JF, Griel Jr LC and Voglmayr JK. 1974. Sperm production of Holstein bulls determined from testicular spermatid reserves, after annulation of rete testis or Vas deferens and by dairy ejaculation. J. Dairy Sci. 57: 93-99.
  3. Arriola J and Foote RH. 1987. Glycerolation and thawing effects on bull spermatozoa frozen in detergent-treated egg yolk and whole egg extenders. J. Dairy Sci. 70:1664-1670.
  4. Chaveiro A, Machado L, Frijters A, Engel B and Woelders H. 2006. Improvement of prarmeters of freezing medium and freezing protocol for bull sperm using two osmotic supports. Theriogenology 65:1875-1890.
  5. Evans G and Maxwell WMC. 1987. Frozen storage of semen. In: Salamon's Artificial Insemination of Sheep and Goats. Butterworths, Wellington, pp. 122-141.
  6. Everett RW and Bean B. 1982. Environmental influences on semen output. J. Dairy Sci. 65:1303-1310.
  7. Everett RW, Bean B and Foote RH. 1978. Sources of variation of semen output. J. Dairy Sci. 61:90-95.
  8. Eyestone WH and First NL. 1985. Co-culture of early cattle embryos to the blastocyst stage with oviducial tissue or in conditioned medium. J. Reprod. Fertil. 85:715-720.
  9. Field MJ, Burns WC and Warnick AC. 1979. Age, season, and breed effects on testicular volume and semen traits in young bulls. J. Anim. Sci. 48:1299-1304.
  10. Flipse RJ and Almquist JO. 1961. Effect of total digestible nutrient intake to four years of age on growth and reproductive development and performance of dairy bull. J. Dairy Sci. 44:905.
  11. Gandhi AP, Lane DK, Gardner DK and Krisher RL. 2000. A single medium supports development of bovine embryos throughout maturation, fertilization and culture. Human Reprod. 5:395-401.
  12. Gardner DK. 1996. Environment of the preimplantation human embryo in vivo: metabolite analysis of oviduct and uterine fluids and metabolism of cumulus cells. Fertility and Sterility 65:349-353.
  13. Garner DL, Johnson LA, Allen CH, Palencia DD and Chambers CS. 1996. Comparison of seminal quality in Holstein bulls as yearlings and mature sires. Theriogenology 45: 923-924.
  14. Hahn J, Foote RH and Seidel GE Jr. 1969. Testicular growth and related sperm output in dairy bulls. J. Anim. Sci. 29: 41-47.
  15. Hoshi H. 2003. In vitro production of bovine embryos and their application for embryo transfer. Theriogenology 59: 675-685.
  16. Kim So-Seob and Park Yong-Soo. 2011. Associations between pedigree, meat quality, and meat quantity of slaughterhouse donor cows and oocyte recovery, embryo development, and pregnancy after embryo transfer. Livestock Sci. 142:42-47
  17. Lim JM, Liou SS and Hansel W. 1996. Intracytoplasmic glutathione concentration and the role of ${\beta}$-mercaptoethanol in preimplantation development of bovine embryos. Theriogenology 46:429-439.
  18. Nagai T. 2001. The improvement of in vitro maturation systems for bovine and porcine oocytes. Theriogenology 55:1291-1301.
  19. Purdy PH. 2006. A review on goat sperm cryopreservation. Small Ruminant Research 63:215-225.
  20. Rosenkrans CF, Zeng GQ, Mcnamara GT, Schoff PK and First NL. 1993. Development of bovine embryos in vitro as affected by energy substrates. Biol. Reprod. 49:459-462.
  21. Sansone G, Nastri MJF and Fabbrocini A. 2000. Storage of buffalo (Bubalus bubalis) semen. Anim. Reprod. Sci. 62: 55-76.
  22. Shiva Shankar Reddy N, Jagan Mohanarao G and Atreja SK. 2010. Effects of adding taurine and trehalose to a tris-based egg yolk extender on buffalo (Bubalus bubalis) sperm quality following cryopreservation. Anim. Reprod. Sci. 119: 183-190.
  23. Vishwanath R and Shannon P. 2000. Storage of bovine semen in liquid and frozen state. Anim. Reprod. Sci. 62:23-53.
  24. Ward F, Enright B, Riaos D, Boland M and Lonergan P. 2002. Optimization of in vitro bovine embryo production: effect of duration of maturation, length of gamete co-incubation, sperm concentration and sire. Theriogenology 57:2105-2117.
  25. Wright RW and Bondioli KR. 1981. Aspects of in vitro fertilization and embryo culture in domestic animals. J. Anim. Sci. 53:702-729.
  26. Yang NS, Lu KH and Gordon I. 1990. In vitro fertilization (IVF) and culture (IVC) of bovine oocytes from stored ovaries. Theriogenology Abst. 33:352.
  27. 김상환, 홍연식, 이호준, 윤종택. 2011. 칡한우 혈액에서 DNA 다양성 분석을 통한 표지 유전자 탐색. 한국발생생물학회지 15: 315-324.
  28. 박연수, 황환섭, 유재원, 김남욱. 2007. 재래 칡소의 정액 특성 및 인공수정에 의한 송아지 모색 분포. 한국동물번식학회지 31:43-48.
  29. 이성수, 양영훈, 강승률, 오운용, 양보석, 고서봉, 오성종, 김규일. 2002. 칡소와 비경흑색 한우의 Melanocortin Receptor1(MCR1) 유전자형 분석. 동물자원과학회지 44:23-30.
  30. 이성수, 정준, 박노형. 2006. 한우 종모우에 있어 정액 채취시의빈대 혹은 의빈우 이용이 정액성상에 미치는 영향. 한국수정란이식학회지 21:95-100.
  31. 이호준, 김상환, 이경태, 윤종택. 2011. 수정란 이식에 의해 생산된 칡소의 모색 발현. 한국발생생물학회지 15:325-329.
  32. 조상래, 김성재, 손준규, 최선호, 최창용, 고응규, 이풍연, 김현종. 2011. 칡소 정액 동결을 위한 AndroMed와 Tris-egg Yolk 희석제의 동결성 비교. 한국수정란이식학회지 26:65-70.
  33. 조상래, 최선호, 최창용, 손준규, 김재범, 김성재, 손동수, 김현종. 2009. AndroMed를 이용한 흑우 동결정액으로 체외수정란 생산 효과. 한국수정란이식학회지 24:207-212.
  34. 최선호, 고민희, 강태영, 조상래, 박용상, 오신애. 2011. 제주 흑우 동결 정액 제조에 있어서 Glycerol의 농도에 따른 생존율 및 정자 첨체 양상의 변화. 한국수정란이식학회지 26:187-193.