Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지

Production of Cloned Miniature Pig by Surrogate Mother Conditions

대리모의 준비 조건 변화를 통한 복제미니돼지의 생산

  • Hur, Chang-Gi (CHO-A Biotechnology Research Institute, CHO-A Pharmaceutical Co. Ltd.) ;
  • Yang, Hae-Young (CHO-A Biotechnology Research Institute, CHO-A Pharmaceutical Co. Ltd.) ;
  • Lee, Eun-Kyeong (CHO-A Biotechnology Research Institute, CHO-A Pharmaceutical Co. Ltd.) ;
  • Han, Joo-Hee (CHO-A Biotechnology Research Institute, CHO-A Pharmaceutical Co. Ltd.) ;
  • Park, Chun-Gyu (CHO-A Biotechnology Research Institute, CHO-A Pharmaceutical Co. Ltd.) ;
  • Shin, Teak-Soon (Department of Animal Science, Pusan National University) ;
  • Lee, Hong-Gu (Department of Animal Science, Pusan National University) ;
  • Kang, Han-Seok (Department of Animal Science, Pusan National University) ;
  • Ahn, Jong-Deok (CHO-A Biotechnology Research Institute, CHO-A Pharmaceutical Co. Ltd.) ;
  • Cho, Seong-Keun (Department of Animal Science, Pusan National University)
  • 허창기 (조아제약(주) 조아생명공학연구소) ;
  • 양혜영 (조아제약(주) 조아생명공학연구소) ;
  • 이은경 (조아제약(주) 조아생명공학연구소) ;
  • 한주희 (조아제약(주) 조아생명공학연구소) ;
  • 박천규 (조아제약(주) 조아생명공학연구소) ;
  • 신택순 (부산대학교 동물생명자원과학과) ;
  • 이홍구 (부산대학교 동물생명자원과학과) ;
  • 강한석 (부산대학교 동물생명자원과학과) ;
  • 안종덕 (조아제약(주) 조아생명공학연구소) ;
  • 조성근 (부산대학교 동물생명자원과학과)
  • Received : 2012.01.05
  • Accepted : 2012.01.20
  • Published : 2012.03.31
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Abstract

Somatic cell nuclear transfer (SCNT) for miniature pig has been developed for xenotransplantation and many other biomedical experiments. However, the efficiency of SCNT is still very low due to many factors. To optimize the surrogate mother condition for improvement of cloned miniature pigs efficiency, we investigated the effect of the status of surrogate mother on pregnancy, farrowed rate in SCNT pigs. After SCNT with mesenchymal stem cells as donor cells, the SCNT embryos were surgically transferred into the oviduct of surrogated pigs. To compare the effects of status of surrogate pigs on pregnancy, surrogate pigs were prepared by artificial abortion at day 20~29 (Group 1), 30~39 (Group 2), and 40~45 (Group 3) of gestation. After SCNT embryos transfer in three different status of surrogate pigs, Group 2 (56.3%) and 3 (55.6%) had significantly ($p$ <0.05) higher the pregnancy rate than group 1 (0%) at day 30 of gestation. The status of ovulation in surrogate pig also was investigated. Post-ovulation status (54.8%) had higher proportion than pre-ovulation status (38.7%) and ovulation status (6.5%). We obtained 19 cloned miniature piglets from seven surrogate gilts and five piglets are living healthy but fourteen piglets died soon after birth or stillbirth. The weights of piglets greatly differ from 254 to 1,296 g. Microsatellite analysis showed that cloned piglets were genetically different from the surrogate mother and cloned piglets were genetically equal to the donor cell. In conclusion, the present result indicates that artificially abortion method can improve the efficiency of pregnancy after SCNT in pigs. This study will provide available method for the further study and application in the field of xenotransplantation.

Keywords

References

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