Oxidative Effects of Isoflurane and Medetomidine - Tiletamine / Zolazepam Combination in Beagle Dogs

비글 견에서 Isoflurane과 Medetomidine - Tiletamine/Zolazepam 병용의 산화효과

  • Choi, Kyeong-Ha (College of Veterinary Medicine, Chungnam National University) ;
  • Lee, Jae-Yeon (College of Veterinary Medicine, Chungnam National University) ;
  • Jeong, Seong-Mok (College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Myung-Cheol (College of Veterinary Medicine, Chungnam National University)
  • Accepted : 2012.02.09
  • Published : 2012.04.30

Abstract

The present study evaluated the effects of different anesthesia techniques on oxidative stress in beagle dogs. Ten dogs were randomly assigned to either total intramuscular anesthesia with medetomidine-tiletamine/zolazepam (MTZ) combination (group T, 40 ${\mu}g/kg$ medetomidine and 2 mg/kg tiletamine/zolazepam) or volatile anesthesia with isoflurane (group I, 2% isoflurane and 100% oxygen). Heart rate, respiratory rate, and rectal temperature for vital signs and the concentration of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) for oxidative stress were measured. SOD activity decreased significantly from baseline anesthesia in both groups ($p$ < 0.05). CAT and GPx activities were also decreased significantly after anesthesia between both groups ($p$ < 0.05). CAT activity decreased significantly from baseline after anesthesia in both groups, but activities of group I were significantly higher compared with group T after anesthesia ($p$ < 0.05). GPx activity in group T decreased significantly from baseline after anesthesia, but activities of group I were significantly higher compare with that of group T 1 hour after the conclusion of anesthesia ($p$ < 0.05). In conclusion, general anesthesia seems to induce oxidative stress, and volatile anesthesia with isoflurane attenuates oxidative injuries in beagle dogs.

이 연구는 비글 견에서 산화스트레스에 대한 서로 다른 마취 방법의 효과를 평가했다. 10마리 견들을 무작위로 medetomidine과 tiletamine/zolazepam(MTZ) combination(그룹 T, 40 ${\mu}g/kg$ medetomidine and 2 mg/kg tiletamine/zolazepam, IM)을 사용한 근육주사 그룹 또는 Isoflurane(그룹 I, 2% isoflurane and 100% oxygen)을 사용한 휘발성 마취 그룹으로 나누었다. Vital sign으로 심박수, 호흡수, 직장체온과 oxidative stress로 superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx)를 측정했다. SOD activity는 두 그룹에서 마취 후 기준 값으로부터 유의성 있게 감소하였다 ($p$ < 0.05). CAT와 GPx activity 또한 마취 후 두 그룹 사이에서 유의성이 있었다 ($p$ < 0.05). CAT activity는 두 그룹에서 마취 후 기준 값으로부터 유의성 있게 감소하였으나, 그룹 I에서는 마취 후 그룹 T의 그것과 비교 시 유의성 있게 높았다 ($p$ < 0.05). 그리고 그룹 T에서 GPx activity는 마취 후 기준 값으로부터 유의성 있 게 감소하였으나, 그룹 I에서는 마취종료 후 1 시간이 되었을 때 그룹 T의 그것과 비교 시 유의성 있게 높았다 ($p$ < 0.05). 결론적으로, 비글 견에서 전신 마취는 산화 스트레스를 유발시키는 경향이 있었으며, isoflurane의 휘발성 마취는 산화 손상을 감소시켰다.

Keywords

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