한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제29권2호
  • /
  • Pages.119-123
  • /
  • 2012
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  • 1598-298X(pISSN)
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  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

비글 견에서 Isoflurane과 Medetomidine - Tiletamine/Zolazepam 병용의 산화효과

Oxidative Effects of Isoflurane and Medetomidine - Tiletamine / Zolazepam Combination in Beagle Dogs

  • Choi, Kyeong-Ha (College of Veterinary Medicine, Chungnam National University) ;
  • Lee, Jae-Yeon (College of Veterinary Medicine, Chungnam National University) ;
  • Jeong, Seong-Mok (College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Myung-Cheol (College of Veterinary Medicine, Chungnam National University)
  • 심사 : 2012.02.09
  • 발행 : 2012.04.30
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초록

이 연구는 비글 견에서 산화스트레스에 대한 서로 다른 마취 방법의 효과를 평가했다. 10마리 견들을 무작위로 medetomidine과 tiletamine/zolazepam(MTZ) combination(그룹 T, 40 ${\mu}g/kg$ medetomidine and 2 mg/kg tiletamine/zolazepam, IM)을 사용한 근육주사 그룹 또는 Isoflurane(그룹 I, 2% isoflurane and 100% oxygen)을 사용한 휘발성 마취 그룹으로 나누었다. Vital sign으로 심박수, 호흡수, 직장체온과 oxidative stress로 superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx)를 측정했다. SOD activity는 두 그룹에서 마취 후 기준 값으로부터 유의성 있게 감소하였다 ($p$ < 0.05). CAT와 GPx activity 또한 마취 후 두 그룹 사이에서 유의성이 있었다 ($p$ < 0.05). CAT activity는 두 그룹에서 마취 후 기준 값으로부터 유의성 있게 감소하였으나, 그룹 I에서는 마취 후 그룹 T의 그것과 비교 시 유의성 있게 높았다 ($p$ < 0.05). 그리고 그룹 T에서 GPx activity는 마취 후 기준 값으로부터 유의성 있 게 감소하였으나, 그룹 I에서는 마취종료 후 1 시간이 되었을 때 그룹 T의 그것과 비교 시 유의성 있게 높았다 ($p$ < 0.05). 결론적으로, 비글 견에서 전신 마취는 산화 스트레스를 유발시키는 경향이 있었으며, isoflurane의 휘발성 마취는 산화 손상을 감소시켰다.

The present study evaluated the effects of different anesthesia techniques on oxidative stress in beagle dogs. Ten dogs were randomly assigned to either total intramuscular anesthesia with medetomidine-tiletamine/zolazepam (MTZ) combination (group T, 40 ${\mu}g/kg$ medetomidine and 2 mg/kg tiletamine/zolazepam) or volatile anesthesia with isoflurane (group I, 2% isoflurane and 100% oxygen). Heart rate, respiratory rate, and rectal temperature for vital signs and the concentration of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) for oxidative stress were measured. SOD activity decreased significantly from baseline anesthesia in both groups ($p$ < 0.05). CAT and GPx activities were also decreased significantly after anesthesia between both groups ($p$ < 0.05). CAT activity decreased significantly from baseline after anesthesia in both groups, but activities of group I were significantly higher compared with group T after anesthesia ($p$ < 0.05). GPx activity in group T decreased significantly from baseline after anesthesia, but activities of group I were significantly higher compare with that of group T 1 hour after the conclusion of anesthesia ($p$ < 0.05). In conclusion, general anesthesia seems to induce oxidative stress, and volatile anesthesia with isoflurane attenuates oxidative injuries in beagle dogs.

키워드

참고문헌

  1. Allaouchiche B, Debon R, Goudable J, Chassard D, Duflo F. Oxidative stress status during exposure to propofol, sevoflurane and desflurane. Anesth Analg 2001; 93: 981-985. https://doi.org/10.1097/00000539-200110000-00036
  2. Caulkett NA, Cattet MR, Cantwell S, Cool N, Olsen W. Anesthesia of wood bison with medetomidine-zolazepam/ tiletamine and xylazine-zolazepam/tiletamine combinations. Can Vet J 2000; 41: 49-53.
  3. Caulkett NA, Cattet MR. Physiological effects of medetomidine- zolazepam-tiletamine immobilization in black bears. J Wildl Dis 1997; 33: 618-622. https://doi.org/10.7589/0090-3558-33.3.618
  4. Ceylan C, Aydilek N, Ipek H. Effects of tiletamine-zolazepam anaesthesia on plasma antioxidative status and some haematological parameters in sheep. Acta Vet Hung 2007; 55: 191-197. https://doi.org/10.1556/AVet.55.2007.2.4
  5. Cuzzocrea S, Riley DP, Caputi AP, Salvemini D. Antioxidant therapy:a new pharmacological approach in shock, inflammation, and ischemia / reperfusion injury. Pharmacol Rev 2001; 53: 135-159.
  6. Durak I, Kavutcu M, Kaçmaz M, Avci A, Horasanli E, Dikmen B, Cimen MY, Oztürk HS. Effects of isoflurane on nitric oxide metabolism and oxidant status of guinea pig myocardium. Acta Anaesthesiol Scand 2001; 45: 119-122. https://doi.org/10.1034/j.1399-6576.2001.450118.x
  7. Durak I, Ozturk HS, Dikmen B, Guven C, Cimen MY, Buyukkocak S, Kacmaz M, Avci A. Isoflurane impairs antioxidant defence system in guinea pig kidney. Can J Anaesth 1999; 46: 797-802. https://doi.org/10.1007/BF03013919
  8. Elsersy H, Sheng H, Lynch JR, Moldovan M, Pearlstein RD, Warner DS.Effects of isoflurane versus fentanyl-nitrous oxide anesthesia on long-term outcome from severe forebrain ischemia in the rat. Anesthesiology 2004; 100: 1160-1166. https://doi.org/10.1097/00000542-200405000-00018
  9. Engelhard K, Werner C, Reeker W, Lu H, Mollenberg O, Mielke L, Kochs E. Desflurane and isoflurane improve neurological outcome after incomplete cerebral ischaemia in rats. Br J Anaesth 1999; 83: 415-421. https://doi.org/10.1093/bja/83.3.415
  10. Gil F, Fiserova-Bergerova V, Altman NH. Hepatic protection from chemical injury by isoflurane. Anesth Analg 1988; 67: 860-867.
  11. Gilgun-Sherki Y, Rosenbaum Z, Melamed E, Offen D. Antioxidant therapy in acute central nervous system injury: current state. Pharmacol Rev 2002; 54: 271-284. https://doi.org/10.1124/pr.54.2.271
  12. Goodyear-Bruch C, Pierce JD. Oxidative stress in critically ill patients. Am J Crit Care 2002; 11: 543-551.
  13. Gutteridge JM, Mitchell J. Redox imbalance in the critically ill. Br Med Bull 1999; 55: 49-75. https://doi.org/10.1258/0007142991902295
  14. Jolly SR, Kane WJ, Bailie MB, Abrams GD, Lucchesi BR. Canine myocardial reperfusion injury. Its reduction by the combined administration of superoxide dismutase and catalase. Circ Res 1984; 54: 277-285. https://doi.org/10.1161/01.RES.54.3.277
  15. Kotani N, Lin CY, Wang JS, et al. Loss of alveolar macrophages during anesthesia and operation in humans. Anesth Analg 1995; 81: 1255-1262.
  16. Kotani N, Takahashi S, Sessler DI, et al. Volatile anesthetics augment expression of proinflammatory cytokines in rat alveolar macrophages during mechanical ventilation. Anesthesiology 1999; 91: 187-197. https://doi.org/10.1097/00000542-199907000-00027
  17. Kudo M, Aono M, Lee Y, Massey G, Pearlstein RD, Warner DS. Absence of direct antioxidant effects from volatile anesthetics in primary mixed neuronal-glial cultures. Anesthesiology 2001; 94: 303-212. https://doi.org/10.1097/00000542-200102000-00021
  18. Kwon YS, Jeong JH, Jang KH, Comparision of tiletamine/ zolazepam, xylazine-tiletamine/zolazepam and medetomidinetiletamine/ zolazepam anesthesia in dogs. J Vet Clin 2003; 20: 33-41.
  19. Kwon YS, Joo EJ, Jang KH, The clinical effectiveness of atipamezole as a medetomidine-tiletamine/zolazepam antagonist in dogs. J Vet Clin 2003; 20: 286-293.
  20. Lee SA, Choi JG, Zuo Z. Volatile anesthetics attenuate oxidative stress-reduced activity of glutamate transporter type 3. Anesth Analg 2009; 109: 1506-1510. https://doi.org/10.1213/ANE.0b013e3181b6709a
  21. Marinovic J, Bosnjak ZJ, Stadnicka A. Distinct roles for sarcolemmal and mitochondrial adenosine triphosphate-sensitive potassium channels in isoflurane-induced protection against oxidative stress. Anesthesiology 2006; 105: 98-104. https://doi.org/10.1097/00000542-200607000-00018
  22. Muir WW, Hubbell RM, Bednarski RM. Handbook of veterinary anesthesia. 4th ed, St Louis: Mosby 2007: 164-176.
  23. Murphy PG, Davies MJ, Columb MO, Stratford N. Effect of propofol and thiopentone on free radical mediated oxidative stress of the erythrocyte. Br J Anaesth 1996; 76: 536-543. https://doi.org/10.1093/bja/76.4.536
  24. Murphy PG, Myers DS, Davies MJ, Webster NR, Jones JG. The antioxidant potential of propofol (2,6-diisopropylphenol). Br J Anaesth 1992; 68: 613-618. https://doi.org/10.1093/bja/68.6.613
  25. Polat B, Albayrak Y, Suleyman B, Dursun H, Odabasoglu F, Yigiter M, Halici Z, Suleyman H. Antiulcerative effect of dexmedetomidine on indomethacin-induced gastric ulcer in rats. Pharmacol Rep 2011; 63: 518-526. https://doi.org/10.1016/S1734-1140(11)70518-7
  26. Sakai H, Sheng H, Yates RB, Ishida K, Pearlstein RD, Warner DS. Isoflurane Provides Long-term Protection against Focal Cerebral Ischemia in the Rat. Anesthesiology 2007; 106: 92-99. https://doi.org/10.1097/00000542-200701000-00017
  27. Shayevitz JR, Varani J, Ward PA, Knight PR. Halothane and isoflurane increase pulmonary artery endothelial cell sensitivity to oxidant-mediated injury. Anesthesiology 1991; 74: 1067-1077. https://doi.org/10.1097/00000542-199106000-00015
  28. Shreeniwas R, Koga S, Karakurum M, Pinsky D, Kaiser E, Brett J, Wolitzky BA, Norton C, Plocinski J, Benjamin W, Kurns DK, Goldestein A, Stern D. Hypoxia-mediated induction of endothelial cell interleukin-1 alpha. An autocrine mechanism promoting expression of leukocyte adhesion molecules on the vessel surface. J Clin Invest 1992; 90: 2333-2339. https://doi.org/10.1172/JCI116122
  29. Sinclair MD. A review of the physiological effects of alpha2-agonists related to the clinical use of medetomidine in small animal practice. Can Vet J 2003; 44: 885-897.
  30. Smith DS, Rehncrona S, Siesjo BK. Inhibitory effects of different barbiturates on lipid peroxidation in brain tissue in vitro: comparison with the effects of promethazine and chlorpromazine. Anesthesiology 1980; 53: 186-194. https://doi.org/10.1097/00000542-198009000-00002
  31. Steffey EP, Howland D Jr. Isoflurane potency in the dog and cat. Am J Vet Res 1977; 38: 1833-1836.
  32. Thurmon JC, Tranquilli WJ, Benson GJ. Considerations for general anesthesia. In: Lumb & Jones' Veterinary anesthesia, 3rd ed. Philadelphia: Williams & Wilkins. 1996: 5-34.
  33. Thurmon JC, Tranquilli WJ, Benson GJ. Preanesthetics and anesthetic adjuncts. In: Lumb & Jones' Veterinary anesthesia, 3rd ed. Philadelphia: Williams & Wilkins. 1996: 183-209.
  34. Trachootham D, Lu W, Ogasawara MA, Nilsa RD, Huang P. Redox regulation of cell survival. Antioxid Redox Signal 2008; 10: 1343-1374. https://doi.org/10.1089/ars.2007.1957
  35. Tsuchiya M, Asada A, Maeda K, Ueda Y, Sato EF, Shindo M, Inoue M. Propofol versus midazolam regarding their antioxidant activities. Am J Respir Crit Care Med 2000; 163: 26-31.
  36. Tsuchiya M, Sato EF, Inoue M, Asada A. Open abdominal surgery increases intraoperative oxidative stress: can it be prevented? Anesth Analg 2008; 107: 1946-1952. https://doi.org/10.1213/ane.0b013e318187c96b
  37. Wattwil LM, Olsson JG. Circulatory effects of isoflurane during acute hypercapnia. Anesth Analg 1987; 66: 1234-1239.
  38. Zweier JL, Talukder MA. The role of oxidants and free radicals in reperfusion injury. Cardiovasc Res 2006; 70: 181-190. https://doi.org/10.1016/j.cardiores.2006.02.025