Journal of Nutrition and Health

  • 제45권2호
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  • Pages.113-120
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  • 2012
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  • 2288-3886(pISSN)
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  • 2288-3959(eISSN)
한국영양학회 (The Korean Nutrition Society)
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실크 단백질의 식이 공급이 아토피 피부염 동물 모델 NC/Nga Mice 표피의 스핑고이드 베이스 및 인산화물 함량 변화에 미치는 영향

Dietary effect of silk protein on epidermal levels of free sphingoid bases and phosphate metabolites in NC/Nga mice

  • 김영애 (경희대학교 동서의학대학원 의학영양학과) ;
  • 송은화 (경희대학교 동서의학대학원 의학영양학과) ;
  • 신경오 (충북대학교 약학대학) ;
  • 이용문 (충북대학교 약학대학) ;
  • 조윤희 (경희대학교 동서의학대학원 의학영양학과)
  • Kim, Young-Ae (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Song, Eun-Hwa (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Shin, Kyoung-Oh (College of Pharmacy, Chungbuk National University) ;
  • Lee, Yong-Moon (College of Pharmacy, Chungbuk National University) ;
  • Cho, Yun-Hi (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
  • 투고 : 2011.12.14
  • 심사 : 2012.02.09
  • 발행 : 2012.04.30
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초록

In our previous studies, dietary supplements of silk protein, sericin, and fibroin, were beneficial for improving epidermal levels of ceramides, which are the major lipids for maintaining the epidermal barrier. In this study, we investigated the dietary effects of silk protein on epidermal levels of free sphingoid bases and their phosphates such as $C_{18}$ sphingosine (So), $C_{18}$ sphinganine (Sa), $C_{18}$ sphingosine-1-phosphate (S1P), and $C_{18}$ sphinganine-1-phosphate (Sa1P), which are either synthetic substrate or degradative metabolites of ceramides. Forty-five male NC/Nga mice, an animal model of atopic dermatitis (AD), were divided into three groups: group CA was an atopic control and fed a control diet, group S was fed a 1% sericin diet, and group F was fed a 1% fibroin diet. Fifteen male BALB/c mice served as group C (control group) and were fed the control diet. All mice were fed with diets and water $ad$ $libitum$ for 10 weeks. Sa in group CA was lower than that in group C, but So in group CA was similar to that in group C. So and Sa were higher in groups S and F than those in group CA; So level was even higher than that in group C, and Sa level was similar to that of group C. The So/Sa ratio in group CA, which is reported to increase in AD, was significantly higher than that of group C. The So/Sa ratio was lower in groups S and F than that in group CA, and decreased further in group F. However, S1P and Sa1P in groups S and F were similar to those in group CA. Taken together, we demonstrated that silk protein, sericin and fibroin dietary supplements, increased So and Sa levels, and decreased the So/Sa ratio.

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참고문헌

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피인용 문헌

  1. Manufacture and Characterization of Silkworm Gland Hydrolysate vol.50, pp.2, 2012, https://doi.org/10.7852/jses.2012.50.2.76
  2. Comparative effect of dietary borage oil and safflower oil on anti-proliferation and ceramide metabolism in the epidermis of essential fatty acid deficient guinea pigs vol.48, pp.4, 2015, https://doi.org/10.4163/jnh.2015.48.4.319