The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Isolation and Characterization of Oligotrophic Bacteria Possessing Induced Systemic Disease Resistance against Plant Pathogens

  • Han, Song-Hee (Institute of Environmentally-Friendly Agriculture, Chonnam National University) ;
  • Kang, Beom-Ryong (Environment-Friendly Agricultural Research Institute, Jeollanamdo Agricultural Research and Extension Services) ;
  • Lee, Jang-Hoon (Institute of Environmentally-Friendly Agriculture, Chonnam National University) ;
  • Kim, Hyun-Jung (Institute of Environmentally-Friendly Agriculture, Chonnam National University) ;
  • Park, Ju-Yeon (Institute of Environmentally-Friendly Agriculture, Chonnam National University) ;
  • Kim, Jeong-Jun (Agricultural Microbiology Team, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Young-Cheol (Institute of Environmentally-Friendly Agriculture, Chonnam National University)
  • Received : 2011.11.18
  • Accepted : 2012.01.03
  • Published : 2012.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

Biocontrol microbes have mainly been screened among large collections of microorganisms $via.$ nutrient-rich $in$ $vitro$ assays to identify novel and effective isolates. However, thus far, isolates from only a few genera, mainly spore-forming bacilli, have been commercially developed. In order to isolate field-effective biocontrol microbes, we screened for more than 200 oligotrophic bacterial strains, isolated from rhizospheres of various soil samples in Korea, which induced systemic resistance against the soft-rot disease caused by $Pectobacterium$ $carotovorum$ SCC1; we subsequently conducted in $planta$ bioassay screening. Two oligotrophic bacterial strains were selected for induced systemic disease resistance against the $Tobacco$ $Mosaic$ $Virus$ and the gray mold disease caused by $Botrytis$ $cinerea$. The oligotrophic bacterial strains were identified as $Pseudomonas$ $manteilii$ B001 and $Bacillus$ $cereus$ C003 by biochemical analysis and the phylogenetic analysis of the 16S rRNA sequence. These bacterial strains did not exhibit any antifungal activities against plant pathogenic fungi but evidenced several other beneficial biocontrol traits, including phosphate solubilization and gelatin utilization. Collectively, our results indicate that the isolated oligotrophic bacterial strains possessing induced systemic disease resistance could provide useful tools as effective biopesticides and might be successfully used as cost-effective and preventive biocontrol agents in the field.

Keywords

References

  1. Castresana, J. 2000. Selection of conserved blocks from multiple alignments for their use in phylogenetic tool. Curr. Opin. Genet. Dev. 8:668-674.
  2. Cho, S. M., Park, J. Y., Han, S. H., Anderson, A. J., Yang, K. Y., McSpadden Gardener, B. B. and Kim, Y. C. 2011. Identification and transcriptional analysis of priming genes in Arabidopsis thaliana induced by root colonization with Pseudomonas chlororaphis O6. Plant Pathol. J. 27:272-279. https://doi.org/10.5423/PPJ.2011.27.3.272
  3. Copping, L. G. 2004. The Manual of Biocontrol Agents. 3rd ed of the BioPesticide Manual. British Crop Protection Council, Alton, England. p. 1-171.
  4. Darrah, P. R. 1991. Models of the rhizosphere. I. Microbial-population dynamics around a root releasing soluble and insoluble carbon. Plant Soil 133:187-199. https://doi.org/10.1007/BF00009191
  5. De Vleesschauwer, D. and Hofte, M. 2009. Rhizobacteria-induced systemic resistance. Adv. Bot. Res. 51:224-281.
  6. Dennis, P. G., Miller, A. J. and Hirsh, P. R. 2010. Are root exudates more important than other sources of rhizodeposits in structuring rhizosphere bacterial communities? FEMS Microbiol. Ecol. 72:313-327. https://doi.org/10.1111/j.1574-6941.2010.00860.x
  7. Fravel, D. 2005. Commercialization and implementation of biocontrol. Annu. Rev. Phytopathol. 43:337-359. https://doi.org/10.1146/annurev.phyto.43.032904.092924
  8. Han, S. H., Anderson, A. J., Yang, K. Y., Cho, B. H., Kim, K. Y., Lee, M. C., Kim, Y. H. and Kim, Y. C. 2006. Multiple determinants influence root colonization and induction of induced systemic resistance by Pseudomonas chlororaphis O6. Mol. Plant Pathol. 7:463-472. https://doi.org/10.1111/j.1364-3703.2006.00352.x
  9. Han, S. H., Kim, C. H., Lee, J. H., Park, J. Y., Cho, S. M., Park, S. K., Kim, K. Y., Krishnan, H. B. and Kim, Y. C. 2008. Inactivation of pqq genes of Enterobacter intermedium 60-2G reduces antifungal activity and induction of systemic resistance. FEMS Microbiol. Lett. 282:140-146. https://doi.org/10.1111/j.1574-6968.2008.01120.x
  10. Kim, Y. C., Jung, H., Kim, K. Y. and Park, S. K. 2008. An effective biocontrol bioformulations against Phytophthora blight of pepper using growth mixtures of combined chitinolytic bacteria under different field conditions. Eur. J. Plant Pathol. 120:373-382. https://doi.org/10.1007/s10658-007-9227-4
  11. Kim, Y. C., Lee, J. H., Bae, Y.-S., Sohn, B.-K. and Park, S. K. 2010. Development of effective environmentally-friendly approaches to control Alternaria blight and anthracnose diseases of Korean ginseng. Eur. J. Plant Pathol. 127:443-450. https://doi.org/10.1007/s10658-010-9610-4
  12. Kim, Y. C., Leveau, J. McSpadden Gardener, B. B., Pierson, E. A., Pierson III, L. S. and Ryu, C-M. 2011. The multifactorial basis for plant health promotion by plant-associated bacteria. Appl. Environ. Microbiol. 77:1548-1555. https://doi.org/10.1128/AEM.01867-10
  13. Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16:111-120. https://doi.org/10.1007/BF01731581
  14. Kloepper, J. W., Leong, J., Teintze, M. and Schroth, M. N. 1980. Enhanced plant growth by siderophores produced by plant growth-promoting rhizobacteria. Nature 286:885-886. https://doi.org/10.1038/286885a0
  15. Kloepper, J. W., Ryu, C.-M. and Zhang, S. 2004. Induced systemic resistance and promotion of plant growth by Bacillus spp. Phytophthology 94:1259-1266. https://doi.org/10.1094/PHYTO.2004.94.11.1259
  16. Kumar, S. Tamura, K. and Nei, M. 2004. MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignments. Brief. Bioinform. 5:150-163. https://doi.org/10.1093/bib/5.2.150
  17. Liu, Y.-H., Huang, C.-J. and Chen, C.-Y. 2008. Evidence of induced systemic resistance against Botrytis elliptica in lily. Phytopathology 98:830-836. https://doi.org/10.1094/PHYTO-98-7-0830
  18. Maloney, P. E., Van Bruggen, A. H. C. and Hu, S. 1997. Bacterial community structure in relation to the carbon environments in lettuce and tomato rhizospheres and in bulk soil. Microb. Ecol. 34:109-171. https://doi.org/10.1007/s002489900040
  19. Martin, P. A. W. and Gundersen-Rindal, D. E. 2008. Microbial combinatorics: a simplified approach for isolating insecticidal bacteria. Biocontrol Sci. Techn. 18:291-305. https://doi.org/10.1080/09583150801899716
  20. Park, J. Y., Han, S. H., Lee, J. H., Han, Y. S., Lee, Y. S., Rong, X., McSpadden Gardener, B. B., Park, H.-S. and Kim, Y. C. 2011. Draft genome sequence of the biocontrol bacterium Pseudomonas putida B001, an oligotrophic bacterium that induces systemic resistance to plant diseases. J. Bacteriol. 193:6795-6796. https://doi.org/10.1128/JB.06217-11
  21. Park, J. Y., Yang, S. Y., Kim, Y. C., Kim, J.-C., Dang, Q. L., Kim, J. J. and Kim, I. S. 2012. Antiviral peptide from Pseudomonas chlororaphis O6 against Tobacco Mosaic Virus (TMV). J. Korean Soc. Appl. Biol. Chem. 55:89-94. https://doi.org/10.1007/s13765-012-0015-2
  22. Raaijmakers, J. M., Leeman, M., van Ooschort, M. M. P., van der Sluis, I., Schippers, B. and Bakker, P. A. H. M. 1995. Doseresponse relationships in biological control of Fusarium wilt of radish by Pseudomonas spp. Phytopathology 85:1075-1081. https://doi.org/10.1094/Phyto-85-1075
  23. Sloan, W. T., Quince, C. and Curtis, T. P. 2008. The uncountables. p 35-54. Chapter 3 in Accessing Uncultivated Microorganisms, from the Environment to Organisms and Genomes and Back. K. Zengler ed. ASM Press: Washington D.C.
  24. Suwa, Y. and Hattori, T. 1984. Effects of nutrient concentration on the growth of soil bacteria. Soil Sci. Plant Nutr. 30:397-403. https://doi.org/10.1080/00380768.1984.10434704
  25. Van Loon, L. C., Bakker, P. A. H. M. and Pierterse, C. M. J. 1998. Systemic resistance induced by rhizosphere bacteria. Annu. Rev. Phytopathol. 36:453-483. https://doi.org/10.1146/annurev.phyto.36.1.453

Cited by

  1. Diverse Gene Cassettes in Class 1 Integrons of Facultative Oligotrophic Bacteria of River Mahananda, West Bengal, India vol.8, pp.8, 2013, https://doi.org/10.1371/journal.pone.0071753
  2. Disease Management in Road Trees and Pepper Plants by Foliar Application of Bacillus spp. vol.22, pp.2, 2016, https://doi.org/10.5423/RPD.2016.22.2.81
  3. Pine sawdust as stimulator of the microbial community in post-arable afforested soil vol.63, pp.3, 2017, https://doi.org/10.1080/03650340.2016.1213816
  4. Draft Genome Sequence of a Chitinase-producing Biocontrol Bacterium Serratia sp. C-1 vol.21, pp.3, 2015, https://doi.org/10.5423/RPD.2015.21.3.222