The Journal of Pediatrics of Korean Medicine (대한한방소아과학회지)

The Association of Korean Oriental Pediatrics (대한한방소아과학회)
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Effect of Perillae Folium Extract on Regulation of Type 1 Allergic Response in RBL-2H3 Cells

자소엽(紫蘇葉) 에탄올 추출물이 RBL-2H3 비만세포에서 제 1형 알레르기 반응 조절에 미치는 효과

  • Gok, Su-Yeong (Department of Pediatrics, College of Oriental Medicine, Dongeui University) ;
  • Yu, Sun-Ae (Department of Pediatrics, College of Oriental Medicine, Dongeui University) ;
  • Lee, Seung-Yeon (Department of Pediatrics, College of Oriental Medicine, Dongeui University)
  • 곡수영 (동의대학교 한의과대학 소아과학교실) ;
  • 유선애 (동의대학교 한의과대학 소아과학교실) ;
  • 이승연 (동의대학교 한의과대학 소아과학교실)
  • Received : 2012.01.27
  • Accepted : 2012.02.20
  • Published : 2012.02.28
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Abstract

Objectives Perillae Folium (PF) has been widely used in Korean herbal medicines used for treatment of acute and chronic inflammatory diseases, such as rhinitis, asthma, and enteritis. In this study, to investigate the protective effect of PF on type 1 allergic response, we determined whether PF inhibits early or late allergic responses. Methods The effect of PF was analyzed by ELISA,. RT-PCR and Western blot in RBL-2H3 cells. Levels of ${\beta}$-hexosaminidase, interleukin (IL)-4 and TNF-${\alpha}$ were measured using enzyme-linked immunosorbent assays (ELISAs). mRNA levels of cytokines and enzymes were analyzed with RT-PCR. Signal transduction was analyzed with Western blot. Results We found that PF suppressed ${\beta}$-hexosaminidase release in RBL-2H3 by the IgE-DNP-HSA stimulation. PF also significantly inhibited enzymes level, such as COX-1, COX-2, iNOS, and HDC2, along with reduced cytokine levels, such as IL-2, IL-3, IL-4, IL-6, IL-13, and TNF-${\alpha}$ in RBL-2H3. In addition, PF suppressed the phospholyation of ERK1/2, JNK1/2, and $I{\kappa}B{\alpha}$. Conclusions Our results indicate that PF protects against type 1 allergic response and exert an anti-inflammatory effect through the inhibition of degranulation and expression of cytokines and enzymes via the suppression of signal transduction.

Keywords

References

  1. 전국 한의과대학 본초학교수 공편저. 본초학. 서울:영림사. 2000:125-6.
  2. 강영성, 이성철, 신호동, 신민교, 김재홍, 송호준. 소엽에 의한 알레르기 천식효과에 대한 연구. 대한본초학회지. 2004;19(3):25-34.
  3. 김승수, 정희재, 정승기, 이형구. 神秘湯 및 加味神秘湯이 Allergy 천식 모델 흰쥐의 BALF내 면역세포 및 혈청 IgE에 미치는 영향에 관한 연구. 대한한의학회지. 2002;23(2):198-210.
  4. 김민수, 박동일. 加味解表二陳湯이 알레르기 喘息白鼠의 呼吸 樣相과 氣管 組織에 미치는 影響. 동의생리병리학회지. 2002;16(3):557-62.
  5. 김성훈, 송호철. 柴胡治喘湯과 補益定喘湯이 알레르기 천식 모델 흰쥐의 BALF내 면역세포 및 조직에 미치는 영향. 동의생리병리학회지. 2005;19(1):106-13.
  6. Metcalfe DD, Kaliner M, Donlon MA. The mast cell. Crit. Rev. Immunol. 1981;3:23-74.
  7. Church MK, Levi-Schaffer F. The human mast cell. J. Allergy Clin. Imm. 1997;99:155-60. https://doi.org/10.1016/S0091-6749(97)70089-7
  8. Chang TW, Shiung YY. Anti-IgE as a mast cell-stabilizing therapeutic agent. The Journal of Allergy and Clinical Immunology. 2006:117(6);1203-12. https://doi.org/10.1016/j.jaci.2006.04.005
  9. Abul K. Abbas, Andrew H. Lichtman, Shiv Pillai. 세포분자 면역학. 제6판. 서울:이퍼블릭. 2008. 191-217, 451-71.
  10. Schwartz LB, Lewis RA, Seldin D, Austen KF. Acid hydrolases and tryptase from secretory granules of dispersed human lung mast cells. J Immunol. 1981;126(4):1290-4.
  11. Marquardt DL, Wasserman SI. Modulation of rat serosal mast cell biochemistry by in vivo dexamethasone administration. J Immunol. 1983;131(2):934-9.
  12. Matsuda H, Morikawa T, Ueda K, Managi H, Yoshikawa M. Structural requirements of flavonoids for inhibition of antigen-Induced degranulation, TNF-alpha and IL-4 production from RBL-2H3 cells. Bioorg Med Chem. 2002;10(10):3123-8. https://doi.org/10.1016/S0968-0896(02)00227-4
  13. 신태용. 한약과 알레르기 질환. 서울:신일북스. 2010: 15-33,92-9.
  14. 안덕균. 원색한국본초도감. 서울:교학사. 2002:46.
  15. 張介賓. 景岳全書. 서울:한미의학. 2006:2461.
  16. 이부균, 국윤범. 東醫寶鑑 中 蘇葉이 主藥으로 配伍된 方劑의 活用에 대한 考察. 대한한의학방제학회지. 2005;13(1):179-94.
  17. 許浚. 對譯東醫寶鑑. 서울:법인문화사. 1999:1749.
  18. 한방소아과 교재편찬위원회 공저. 한방소아청소년의학. 서울:의성당. 2010:38-9.
  19. 김영휘, 엄상화, 김우경. 2009년 학동 전기 소아에서 알레르기질환의 유병률과 위험인자. 소아알레르기호흡기학회지. 2011;21(3):165-75.
  20. 김정미, 김대중, 김태혁, 백종미, 김현숙, 최면. 감초추출물이 RBL-2H3 비만세포에서 $\beta$-hexosaminidase 분비 및 Th2 cytokine mRNA 발현에 미치는 효과. 韓藥作誌. 2010;18(4):231-7.
  21. Iikura M, Yamaguchi M, Hirai K, Miyamasu M, Yamada H, Nakajima T, Fujisawa T, Ra C, Morita Y, Yamamoto K. Regulation of surface FcepsilonRI expression on human eosinophils by IL-4 and IgE. Int Arch Allergy Immunol. 2001;124:470-7. https://doi.org/10.1159/000053782
  22. 이중달. 그림으로 설명한 병리학. 서울:고려의학. 1991: 29.
  23. Chai OH, Han EH, Lee HK, Song CH. Mast cells play a key role in Th2 cytokine-dependent asthma model through production of adhesion molecules by liberation of TNF-$\alpha$. Exp Mol Med. 2011;43(1):35-43. https://doi.org/10.3858/emm.2011.43.1.004
  24. Taketomi Y, Murakami M. Lipid networks in mast cell biology. Yakugaku Zasshi. 2001;131(1):73-84.
  25. 이일수. 시호의 생쥐 소교세포에서 지질다당질에 의한 cyclooxygenase-2와 inducible nitric oxide synthase 발현 억제작용. 경원대 대학원 석사학위논문. 2006.
  26. Deo SS, Mistry KJ, Kakade AM, Niphadkar PV. Role played by Th2 type cytokines in IgE mediated allergy and asthma. Lung India. 2010;27(2):66-71. https://doi.org/10.4103/0970-2113.63609