Animal cells and systems

  • 제16권1호
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  • Pages.50-56
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  • 2012
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  • 1976-8354(pISSN)
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  • 2151-2485(eISSN)
한국통합생물학회 (The Korean Society for Integrative Biology)
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Development and characterization of microsatellite markers for an endangered species, $Epinephelus$ $bruneus$, to establish a conservation program

  • An, Hye-Suck (New Strategy Research Center, National Fisheries Research and Development Institute) ;
  • Kim, Jae-Woo (New Strategy Research Center, National Fisheries Research and Development Institute) ;
  • Lee, Jang-Wook (New Strategy Research Center, National Fisheries Research and Development Institute) ;
  • Kim, Shin-Kwon (New Strategy Research Center, National Fisheries Research and Development Institute) ;
  • Lee, Bae-Ik (New Strategy Research Center, National Fisheries Research and Development Institute) ;
  • Kim, Dae-Jung (New Strategy Research Center, National Fisheries Research and Development Institute) ;
  • Kim, Yi-Cheong (New Strategy Research Center, National Fisheries Research and Development Institute)
  • 투고 : 2011.04.29
  • 심사 : 2011.07.19
  • 발행 : 2012.02.28
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초록

Kelp grouper ($Epinephelus$ $bruneus$ Bloch 1793) is a commercially important fish in Korea. In recent years, the catch of kelp grouper in the coastal waters of Korea has significantly declined. Despite its importance, little is known about its genetic diversity and conservation efforts are hampered. In this study, we isolated and characterized 12 microsatellite loci using an enrichment method based on magnetic/biotin capture of microsatellite sequences from a size-selected genomic library. All loci were readily amplified and contained TG/CA denucleotide repeats. To characterize each locus, 30 individuals from a natural E. bruneus population in the coastal waters of Jeju Island, Korea, were genotyped. All loci except three, KEm118, KEm154, and KEm219, were polymorphic, with an average of 8.1 alleles per locus (range 2-18). The mean observed and expected heterozygosities were 0.47 (range 0.19-1.00) and 0.61 (range 0.29-0.92), respectively. A significant deviation from Hardy-Weinberg equilibrium was observed at three loci (KEm134, KEm184, and KEm283). These findings will be useful for effective monitoring and management of genetic variation of kelp grouper as well as for the implementation of a fisheries conservation program.

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  1. When physical oceanography meets population genetics: The case study of the genetic/evolutionary discontinuity in the endangered goliath grouper (Epinephelus itajara; Perciformes: Epinephelidae) with vol.56, pp.None, 2012, https://doi.org/10.1016/j.bse.2014.06.004
  2. Microsatellite marker set for genetic diversity assessment of primitive Chitala chitala (Hamilton, 1822) derived through SMRT sequencing technology vol.46, pp.1, 2012, https://doi.org/10.1007/s11033-018-4414-2