Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
권호 표지

Effect of Suppressing the Activation of Macrophage Migration Inhibitory Factor by $Sambucus$ $williamsii$ $H_{ANCE}$ Extract & Pharmacopuncture Solution on Type II Collagen-induced Arthritis

접골목(接骨木)추출물 및 약침액에 의한 MIF 활성 조절능이 생쥐의 제2형 Collagen 유발 관절염에 미치는 영향

  • Lee, Dong-Gun (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University) ;
  • Kim, Eun-Jung (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University) ;
  • Lee, Eun-Sol (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University) ;
  • Wang, Kai-Hsia (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University) ;
  • Cho, Hyun-Seok (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University) ;
  • Lee, Seung-Deok (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University) ;
  • Kim, Kap-Sung (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University) ;
  • Kim, Kyung-Ho (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University)
  • 이동건 (동국대학교 한의과대학 침구학교실) ;
  • 김은정 (동국대학교 한의과대학 침구학교실) ;
  • 이은솔 (동국대학교 한의과대학 침구학교실) ;
  • 왕개하 (동국대학교 한의과대학 침구학교실) ;
  • 조현석 (동국대학교 한의과대학 침구학교실) ;
  • 이승덕 (동국대학교 한의과대학 침구학교실) ;
  • 김갑성 (동국대학교 한의과대학 침구학교실) ;
  • 김경호 (동국대학교 한의과대학 침구학교실)
  • Received : 2012.02.06
  • Accepted : 2012.02.13
  • Published : 2012.02.20
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Abstract

Objectives : The purpose of this study is to evaluate effect of suppressing the expression of cyclo-oxygenase-type-2 (COX-2) as a consequence of inhibition macrophage migration inhibitory factor (MIF) activation by $Sambucus$ $williamsii$ $Hance$ (SWH) pharmacopunctureon rheumatoid arthritis (RA). Methods : In vitro test, synoviocytes extracted from type II collagen-induced arthritis (CIA) mouse's knee joint were cultivated After that, each well of synoviocytes was mixed with the extract of SWH at the dosage of $0.4mg/m{\ell}$, $0.6mg/m{\ell}$, $0.8mg/m{\ell}$, and $1.0mg/m{\ell}$ respectively, and cultivated for 24 hours after the addition. Reverse transcriptase - polymerase chain reaction (RT-PCR) is used to investigate the expression of MIF, Tumor necrosis factor (TNF)-${\alpha}$, COX-2 mRNA. $In$ $vivo$ test, thirty DBA female mice were used, and each ten mice were allocated into three group; normal group, CIA-elicitated group, and group treated with SWH pharmacopuncture on it's the point of $ST_{35}$ after CIA elicitation. It is investigated that change of mice foot thickness, histologic change of sliced synovial joint of mouse, and extent change of MIF, TNF-${\alpha}$, COX-2 in synovial membrane. Results : $In$ $vitro$ test, the expressions of cytokine(MIF, TNF-${\alpha}$, COX-2) mRNAs related to RA were dose-dependent decreased. In the SWH pharmacopuncture group, foot thickness and histologic change of sliced synovial joint were decreased comparing with CIA-elicitated group's change. In the SWH pharmacopuncture group, the suppression of MIF, TNF-${\alpha}$, COX-2 in synovial membrane was clearly shown comparing with CIA-elicitated group's change. Conclusions : It might be suggested that SWH pharmacopuncture mitigate tissue damage originated from rheumatoid arthritis by suppressing the expression of COX-2 as a consequence of inhibition MIF activation.

Keywords

References

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