Journal of Acupuncture Research

  • 제29권1호
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  • Pages.75-87
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  • 2012
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  • 2586-288X(pISSN)
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  • 2586-2898(eISSN)
대한침구의학회 (Korean Acupuncture & Moxibustion Medicine Society)
권호 표지

농도별 봉독약침이 생쥐의 Type II Collagen 유발 관절염에 미치는 영향

The Effect of Varying Concentrations of Bee Venom Pharmacoupuncture Treatments on Type II Collagen Induced Arthritis in Mice

  • 이승우 (동국대학교 한의과대학 침구학교실) ;
  • 김유종 (동국대학교 한의과대학 침구학교실) ;
  • 김은정 (동국대학교 한의과대학 침구학교실) ;
  • 이승덕 (동국대학교 한의과대학 침구학교실) ;
  • 김갑성 (동국대학교 한의과대학 침구학교실) ;
  • 윤종화 (동국대학교 한의과대학 침구학교실)
  • Lee, Sung-Woo (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University) ;
  • Kim, Yu-Jong (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University) ;
  • Kim, Eun-Jung (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University) ;
  • Lee, Seung-Deok (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University) ;
  • Kim, Kap-Sung (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University) ;
  • Yoon, Jong-Hwa (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongguk University)
  • 투고 : 2012.02.06
  • 심사 : 2012.02.10
  • 발행 : 2012.02.20
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초록

Objectives : The purpose of this study is to inquire into the effect of different concentrations of bee venom pharmacopuncture to inhibit genesis of pro-inflammatory cytokines and to inhibit nuclear factor (NF)-${\kappa}B$ activation on type II collagen induced arthritis. Methods : The experiment was divided into category of the normal group (NOR)-no treated group, control group (CON)-CIA (collagen induced arthritis) induced group, and 4,000 : 1 bee venom group (BV-L)- 4000:1 bee venom pharmacopuncture treated group after CIA, and 2000:1 bee venom group (BV-H)- 2,000 : 1 Bee venom pharmacopuncture treated group after CIA. RA was induced in the mice via injecting $50{\mu}{\ell}$ C II mixed CFA. The bee venom pharmacopuncture was applied on $ST_{35}$ for 19 days from the 3rd day of RA inducement. To research the effect on the expression of IKK ($I{\kappa}B$ kinase), iNOS (inducible nitric oxide synthase) & COX-2 (cyclooxygenase-2) mRNA, RT-PCR was performed on synovial membrane cells from the knee joint of CIA mice. Results : The PMA-induced $I{\kappa}B$ kinase (IKK), inducible nitric oxide synthase (iNOS) and cyclooxygenase -2 (COX-2) mRNA expression were dose-dependantly decreased in bee venom treated with synoviocytes. In mice treated with bee venom pharmacopuncture, foot thickness and the damage of synovial membranes of the joint was lessened, and the activation of RA-related pro-inflammatory cytokines such as MIF, TNF-${\alpha}$ and MMP-9 was significantly decreased. The activation of iNOS and COX-2 was suppressed by the inhibition of NF-${\kappa}B$. In addition, each data was shown that 2,000 : 1 bee venom pharmacopuncture was more effective than 4,000 : 1 bee venom pharmacopuncture. Conclusions : It is speculated that bee venom pharmacopuncture has the therapeutic effect of palliating the damage of the synovial membrane and inflammation on RA by suppressing of NF-${\kappa}B$ activation.

키워드

참고문헌

  1. 대한정형외과학회. 정형외과학. 서울 : 최신의학사. 1999 : 175-89.
  2. 최홍식, 권오성, 이준희, 강윤호. 역절풍에 관한 연구. 서울 : 동국대 한의학연구소논문집. 1996 ; 5 : 97-129.
  3. 이홍석, 이재동, 고형균. 최근 10년간 국내의 봉독 관련 연구에 대한 고찰. 2003 ; 20(3) : 154-65.
  4. 김태우, 최도영, 이재동. 봉독약침이 제2형 콜라겐 유도 관절염에 미치는 영향. 대한침구학회지. 2002 ; 19(2) : 92-104.
  5. 이상훈, 이현종, 백용현, 김수영, 박재경, 홍승재, 양형인, 김건식, 이재동, 최도영, 이두익, 이윤호. 봉독약침이 류마티스 관절염 환자의 관절 통증, 종창 및 급성 염증 반응에 미치는 영향. 대한침구학회지. 2003 ; 20(2) : 77-84.
  6. 김호연. 류마티스관절염치료의 최근경향. 대한내과학회지. 1996 ; 5(4) : 271-9.
  7. Lipsky PE. Rheumatoid arthritis. Harrison's principle of internal medicine, 16th ed. New York : McGraw Hill. 2005 ; 1968-77.
  8. Leo AB Joosten, Monique MA Helsen, Fons AJ van de Loo, Wim B van DE Bern. Anticytokine treatment of established type II collagen-induced arthritis in DBA/a mice. Arthritis Rheum. 1996 ; 39(5) : 797-809. https://doi.org/10.1002/art.1780390513
  9. Morand EF. New therapeutic target in inflammatory disease : macrophage migration inhibitory factor. J Internal medicine. 2005 ; 35 : 419-26. https://doi.org/10.1111/j.1445-5994.2005.00853.x
  10. Marc Feldmann, Fionula M Brennan and Ravinder N Maini. Role of cytokines in rheumatoid arthritis. Annu Rev Immunol. 1996 ; 14 : 397-440. https://doi.org/10.1146/annurev.immunol.14.1.397
  11. Sampey AV, Hall PH, Mitchell RA, Metz CN, Morand EF. Regulation of synoviocytes phospholipase A2 and cyclooxygenase 2 by macrophage migration inhibitory factor. Arthritis Rheum. 2001 ; 44 : 1273-80. https://doi.org/10.1002/1529-0131(200106)44:6<1273::AID-ART219>3.0.CO;2-8
  12. Burrage PS, Mix KS, Brinckerhoff CE. Matrix metalloproteinases ; role in arthritis. Front Biosci. 2006 ; 11 : 529-43. https://doi.org/10.2741/1817
  13. Matthew H Ornstein and Kirk Sperber. The antiinflammatory and antiviral effects of hydroxychloroquine in two patients with acquired immunodeficiency syndrome and active inflammatory arthritis. Arthritis Rheum. 1996 ; 39(1) : 157-61. https://doi.org/10.1002/art.1780390122
  14. Pincus T, Marcum SB, Collahan LF. Longterm drug theraphy for rheumatoid arthritis in seven rheumatology private practice. II. Second line drugs and prednisone. J Rheumatol. 1992 ; 19 : 1885-94.
  15. 홍원식. 校勘直譯 黃帝內經靈樞. 서울 : 傳統文化 硏究院. 1994 : 234-7.
  16. 黃文東. 實用中醫內科學. 上海 : 上海科學技術出版社. 1986 : 566-9.
  17. 舍岩. 舍岩道人鍼灸要訣. 서울 : 행림출판사 : 1982 : 117-122.
  18. 全國韓醫科大學 鍼灸經穴學敎室 編. 鍼灸學 上. 서울 : 集文堂. 1998 : 381-2.
  19. 윤현민, 안창범, 조은진. 봉침요법에 대한 고찰 및 국내 연구동향. 동의대학교 한의학연구소. 2001 ; 5 : 23-42.
  20. Suar JM, Cremer MA, Townes AS and Kang AH. Type II collagen induced arthritis in rats : Transfer with serum and evidence that IgG anticollagen antibodies can cause arthritis. J Exp Med. 1982 ; 155 : 1-16. https://doi.org/10.1084/jem.155.1.1
  21. Trentham DE, Townes AS, Kang AH. Autoimmunity to type II collagen : An experimental model of arthritis. J Exp Med. 1977 ; 146 : 857-68. https://doi.org/10.1084/jem.146.3.857
  22. 박동준 外. 흰 쥐의 제2형 collagen유발 관절염의 IgG 항 collagen 항체의 변화. 대한내과학회지. 1991 ; 41(13) : 423-8.
  23. Carlene T, Luis AD, Nora GS, Lan LL, Antia HK, Raj M, Brian JN, Leslie JC, David AF. Responsive of human T lymphocytes to bacterial superantigens presented by cultured rheumatoid arthritis synoviocytes. Arthritis & rheumatism. 1996 ; 39(1) : 125-36. https://doi.org/10.1002/art.1780390117
  24. Mizel SB, Dayer JM, Krane SM, Mergenhagen SE. Stimulation of rheumatoid synovial cell collagenase and prostaglandin production by partially purified lymphocyte-activating factor (interleukin 1). Proc Natl Acad Sci USA. 1981 ; 78(4) : 2474-7. https://doi.org/10.1073/pnas.78.4.2474
  25. Pettipher ER, Higgs GA, Henderson B. Interleukin 1 induces leukocyte infiltration and cartilage proteoglycan degradation in the synovial joint. Proc Natl Acad Sci USA. 1986 ; 83(22) : 8749-53. https://doi.org/10.1073/pnas.83.22.8749
  26. Saklatvala J. Interleukin 1: purification and biochemical aspects of its action on cartilage. J Rheumatol. 1987 May ; 14 Spec No : 52-4.
  27. Bunning R, Crawford A, Richardson H, Opendakker G, van Damme J, and Russel R. IL-1 preferentially stimulates the production of tissue-type plasminogen activator by human articular chondrocytes. Biochem Biophys Acta. 1987 ; 924 : 437-49.
  28. Corsini E, Sangha N, Feldman SR. Epidermal stratification reduces the effects of UVB (but not UVA) on keratinocyte cytokine production and cytotoxicity. Photodermatol Photoimmunol Photomed. 1997 ; 13(4) : 147-52. https://doi.org/10.1111/j.1600-0781.1997.tb00219.x
  29. Yarosh D, Both D, Kibitel J, Anderson C, Elmets C, Brash D, Brown D. Regulation of TNFalpha production and release in human and mouse keratinocytes and mouse skin after UV-B irradiation. Photodermatol Photoimmunol Photomed. 2000 ; 16(6) : 263-70. https://doi.org/10.1034/j.1600-0781.2000.160606.x
  30. Tanaka K, Hasegawa J, Asamitsu K, Okamoto T. Prevention of the ultraviolet B-mediated skin photoaging by a nuclear factor kappaB inhibitor, parthenolide. J Pharmacol Exp Ther. 2005 ; 315(2) : 624-30. https://doi.org/10.1124/jpet.105.088674
  31. Baeuerle PA and Baltimore D. NF-${\kappa}B$ - Ten years after. Cell. 1996 ; 87 : 13-20. https://doi.org/10.1016/S0092-8674(00)81318-5
  32. Baeuerle PA. $I{\kappa}B$-NF-${\kappa}B$ structure: at the interface of inflammation control. Cell. 1998 ; 95 : 729-31. https://doi.org/10.1016/S0092-8674(00)81694-3
  33. Anggard E. Nitric oxide: mediator, murderer, and medicine. Lancet. 1994 ; 9 : 1199-206.
  34. Radi R, Beckman JS, Bush KM, Freeman BA. Peroxynitrite-induced membrane lipid peroxidation: the cytotoxic potential of superoxide and nitric oxide. Arch Biochem Biophys. 1991 ; 288(2) : 481-7. https://doi.org/10.1016/0003-9861(91)90224-7
  35. Villa LM, Salas E, Darley-Usmar VM, Radomski MW, Moncada S. Peroxynitrite induces both vasodilatation and impaired vascular relaxation in the isolated perfused rat heart. Proc Natl Acad Sci USA. 1994 ; 91(26) : 12383-7. https://doi.org/10.1073/pnas.91.26.12383
  36. Groszmann RJ. Hyperdynamic state in chronic liver diseases. J Hepatol. 1993 ; 17(2) : S38-40. https://doi.org/10.1016/S0168-8278(05)80454-4
  37. HJ RothkOtter, R Pabst and M Bailey. Lymphocyte migration in the intestinal mucosa : entry, transit and emigration of lymphoid cells and the influence of antigen. Vetrinary Immuno immunopath. 1999 ; 72 : 157-65. https://doi.org/10.1016/S0165-2427(99)00128-2
  38. Tsujii M, Kawano S, Tsuji S, Sawaoka H, Hori M, DuBois RN. Cyclooxygenase regulates angiogenesis induced by colon cancer cells. Cell. 1998 ; 93(5) : 705-16. https://doi.org/10.1016/S0092-8674(00)81433-6