Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Outcomes of preimplantation genetic diagnosis using either zona drilling with acidified Tyrode's solution or partial zona dissection

  • Kim, Hyun Jung (Institute of Fertility and Genetics, Hamchoon Women's Clinic) ;
  • Kim, Chung Hyon (Institute of Fertility and Genetics, Hamchoon Women's Clinic) ;
  • Lee, Soo Min (Institute of Fertility and Genetics, Hamchoon Women's Clinic) ;
  • Choe, Seung Ah (Institute of Fertility and Genetics, Hamchoon Women's Clinic) ;
  • Lee, Joong Yeup (Institute of Fertility and Genetics, Hamchoon Women's Clinic) ;
  • Jee, Byung Chul (Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital) ;
  • Hwang, Doyeong (Institute of Fertility and Genetics, Hamchoon Women's Clinic) ;
  • Kim, Ki Chul (Institute of Fertility and Genetics, Hamchoon Women's Clinic)
  • Received : 2012.08.01
  • Accepted : 2012.09.14
  • Published : 2012.09.30
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Abstract

Objective: To review the outcomes of preimplantation genetic diagnosis (PGD) using zona drilling with acid Tyrode's solution (chemical zona pellucida drilling, chemical ZD) and those of partial zona dissection (PZD). Methods: Clinical outcomes of seventy-one couples undergoing 85 PGD cycles from January 2005 to December 2010 were included. Blastocyst formation and the hatching rate, clinical pregnancy rate, ongoing pregnancy rate, implantation rate, and fetal gender ratio of the PZD and chemical ZD groups were compared. Results: Application of PZD resulted in a significantly higher rate of clinical pregnancy (40.7% vs. 15.4%, p=0.022), ongoing pregnancy (35.6% vs. 11.5%, p=0.023), and implantation (18.1% vs. 5.7%, p=0.007) compared with chemical ZD. Among non-transferred embryos, the rate of blastocyst formation on day 5 (49.1% vs. 39.5%, p=0.016) and hatching on day 6 (47.2% vs. 26.5%, p<0.001) were also significantly higher in the PZD group. Conclusion: The mechanical zona dissection (ZD) method showed better outcomes than chemical ZD in terms of the blastocyst development and pregnancy rate. In this study, the fact that chemical ZD was conducted in different period from mechanical method should be considered in interpreting the result.

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References

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